Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens

T. Ikegami, K. Shirota, K. Goto, A. Takakura, T. Itoh, S. Kawamura, Y. Une, Y. Nomura, K. Fujiwara

Research output: Contribution to journalArticle

19 Scopus citations

Abstract

Dual infection by Clostridium piliforme and feline panleukopenia virus (FPLV) was found in three kittens. In all cases, we found focal necrosis and desquamation of epithelial cells with occasional neutrophil infiltration in the large intestine. Large filamentous bacilli and spores were observed in the epithelium by using the Warthin-Starry method. Electron microscopy revealed the vegetative forms with characteristic peritrichous flagella and spore forms. Immunohistochemically, these bacilli showed a positive reaction with mouse antisera against the RT and MSK C. piliforme strains. Polymerase chain reaction (PCR) using cecum specimens demonstrated the 196-bp band specific to C. piliforme 16S rRNA. All three kittens were also diagnosed as FPLV-infected on the basis of the characteristic mucosal lesions, including intranuclear inclusions and PCR study for the FPLV genomic DNA. The PCR techniques are useful for confirming the C. piliforme and FPLV infection in spontaneous cases.

Original languageEnglish (US)
Pages (from-to)613-615
Number of pages3
JournalVeterinary Pathology
Volume36
Issue number6
DOIs
StatePublished - Jan 1 1999
Externally publishedYes

Keywords

  • Cats
  • Clostridium piliforme
  • Colitis
  • Feline panleukopenia virus
  • PCR
  • Tyzzer's disease

ASJC Scopus subject areas

  • veterinary(all)

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  • Cite this

    Ikegami, T., Shirota, K., Goto, K., Takakura, A., Itoh, T., Kawamura, S., Une, Y., Nomura, Y., & Fujiwara, K. (1999). Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens. Veterinary Pathology, 36(6), 613-615. https://doi.org/10.1354/vp.36-6-613