Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens

Tetsuro Ikegami, K. Shirota, K. Goto, A. Takakura, T. Itoh, S. Kawamura, Y. Une, Y. Nomura, K. Fujiwara

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Dual infection by Clostridium piliforme and feline panleukopenia virus (FPLV) was found in three kittens. In all cases, we found focal necrosis and desquamation of epithelial cells with occasional neutrophil infiltration in the large intestine. Large filamentous bacilli and spores were observed in the epithelium by using the Warthin-Starry method. Electron microscopy revealed the vegetative forms with characteristic peritrichous flagella and spore forms. Immunohistochemically, these bacilli showed a positive reaction with mouse antisera against the RT and MSK C. piliforme strains. Polymerase chain reaction (PCR) using cecum specimens demonstrated the 196-bp band specific to C. piliforme 16S rRNA. All three kittens were also diagnosed as FPLV-infected on the basis of the characteristic mucosal lesions, including intranuclear inclusions and PCR study for the FPLV genomic DNA. The PCR techniques are useful for confirming the C. piliforme and FPLV infection in spontaneous cases.

Original languageEnglish (US)
Pages (from-to)613-615
Number of pages3
JournalVeterinary Pathology
Volume36
Issue number6
StatePublished - 1999
Externally publishedYes

Fingerprint

Feline Panleukopenia Virus
Clostridium piliforme
Clostridium Infections
Enterocolitis
enterocolitis
Carnivore protoparvovirus 1
kittens
polymerase chain reaction
Spores
infection
Polymerase Chain Reaction
Bacillus
spores
Intranuclear Inclusion Bodies
Flagella
Neutrophil Infiltration
Cecum
Large Intestine
large intestine
Virus Diseases

Keywords

  • Cats
  • Clostridium piliforme
  • Colitis
  • Feline panleukopenia virus
  • PCR
  • Tyzzer's disease

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Ikegami, T., Shirota, K., Goto, K., Takakura, A., Itoh, T., Kawamura, S., ... Fujiwara, K. (1999). Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens. Veterinary Pathology, 36(6), 613-615.

Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens. / Ikegami, Tetsuro; Shirota, K.; Goto, K.; Takakura, A.; Itoh, T.; Kawamura, S.; Une, Y.; Nomura, Y.; Fujiwara, K.

In: Veterinary Pathology, Vol. 36, No. 6, 1999, p. 613-615.

Research output: Contribution to journalArticle

Ikegami, T, Shirota, K, Goto, K, Takakura, A, Itoh, T, Kawamura, S, Une, Y, Nomura, Y & Fujiwara, K 1999, 'Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens', Veterinary Pathology, vol. 36, no. 6, pp. 613-615.
Ikegami, Tetsuro ; Shirota, K. ; Goto, K. ; Takakura, A. ; Itoh, T. ; Kawamura, S. ; Une, Y. ; Nomura, Y. ; Fujiwara, K. / Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens. In: Veterinary Pathology. 1999 ; Vol. 36, No. 6. pp. 613-615.
@article{bd80bd51776d4d9d927a183a509c9b9c,
title = "Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens",
abstract = "Dual infection by Clostridium piliforme and feline panleukopenia virus (FPLV) was found in three kittens. In all cases, we found focal necrosis and desquamation of epithelial cells with occasional neutrophil infiltration in the large intestine. Large filamentous bacilli and spores were observed in the epithelium by using the Warthin-Starry method. Electron microscopy revealed the vegetative forms with characteristic peritrichous flagella and spore forms. Immunohistochemically, these bacilli showed a positive reaction with mouse antisera against the RT and MSK C. piliforme strains. Polymerase chain reaction (PCR) using cecum specimens demonstrated the 196-bp band specific to C. piliforme 16S rRNA. All three kittens were also diagnosed as FPLV-infected on the basis of the characteristic mucosal lesions, including intranuclear inclusions and PCR study for the FPLV genomic DNA. The PCR techniques are useful for confirming the C. piliforme and FPLV infection in spontaneous cases.",
keywords = "Cats, Clostridium piliforme, Colitis, Feline panleukopenia virus, PCR, Tyzzer's disease",
author = "Tetsuro Ikegami and K. Shirota and K. Goto and A. Takakura and T. Itoh and S. Kawamura and Y. Une and Y. Nomura and K. Fujiwara",
year = "1999",
language = "English (US)",
volume = "36",
pages = "613--615",
journal = "Veterinary Pathology",
issn = "0300-9858",
publisher = "SAGE Publications Ltd",
number = "6",

}

TY - JOUR

T1 - Enterocolitis associated with dual infection by Clostridium piliforme and feline panleukopenia virus in three kittens

AU - Ikegami, Tetsuro

AU - Shirota, K.

AU - Goto, K.

AU - Takakura, A.

AU - Itoh, T.

AU - Kawamura, S.

AU - Une, Y.

AU - Nomura, Y.

AU - Fujiwara, K.

PY - 1999

Y1 - 1999

N2 - Dual infection by Clostridium piliforme and feline panleukopenia virus (FPLV) was found in three kittens. In all cases, we found focal necrosis and desquamation of epithelial cells with occasional neutrophil infiltration in the large intestine. Large filamentous bacilli and spores were observed in the epithelium by using the Warthin-Starry method. Electron microscopy revealed the vegetative forms with characteristic peritrichous flagella and spore forms. Immunohistochemically, these bacilli showed a positive reaction with mouse antisera against the RT and MSK C. piliforme strains. Polymerase chain reaction (PCR) using cecum specimens demonstrated the 196-bp band specific to C. piliforme 16S rRNA. All three kittens were also diagnosed as FPLV-infected on the basis of the characteristic mucosal lesions, including intranuclear inclusions and PCR study for the FPLV genomic DNA. The PCR techniques are useful for confirming the C. piliforme and FPLV infection in spontaneous cases.

AB - Dual infection by Clostridium piliforme and feline panleukopenia virus (FPLV) was found in three kittens. In all cases, we found focal necrosis and desquamation of epithelial cells with occasional neutrophil infiltration in the large intestine. Large filamentous bacilli and spores were observed in the epithelium by using the Warthin-Starry method. Electron microscopy revealed the vegetative forms with characteristic peritrichous flagella and spore forms. Immunohistochemically, these bacilli showed a positive reaction with mouse antisera against the RT and MSK C. piliforme strains. Polymerase chain reaction (PCR) using cecum specimens demonstrated the 196-bp band specific to C. piliforme 16S rRNA. All three kittens were also diagnosed as FPLV-infected on the basis of the characteristic mucosal lesions, including intranuclear inclusions and PCR study for the FPLV genomic DNA. The PCR techniques are useful for confirming the C. piliforme and FPLV infection in spontaneous cases.

KW - Cats

KW - Clostridium piliforme

KW - Colitis

KW - Feline panleukopenia virus

KW - PCR

KW - Tyzzer's disease

UR - http://www.scopus.com/inward/record.url?scp=0033227074&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0033227074&partnerID=8YFLogxK

M3 - Article

C2 - 10568445

AN - SCOPUS:0033227074

VL - 36

SP - 613

EP - 615

JO - Veterinary Pathology

JF - Veterinary Pathology

SN - 0300-9858

IS - 6

ER -