Enthalpy-entropy compensation in biomolecular halogen bonds measured in DNA junctions

Megan Carter, Andrea Regier Voth, Matthew R. Scholfield, Brittany Rummel, Lawrence Sowers, P. Shing Ho

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Interest in noncovalent interactions involving halogens, particularly halogen bonds (X-bonds), has grown dramatically in the past decade, propelled by the use of X-bonding in molecular engineering and drug design. However, it is clear that a complete analysis of the structure-energy relationship must be established in biological systems to fully exploit X-bonds for biomolecular engineering. We present here the first comprehensive experimental study to correlate geometries with their stabilizing potentials for fluorine (F), chlorine (Cl), bromine (Br), or iodine (I) X-bonds in a biological context. For these studies, we determine the single-crystal structures of DNA Holliday junctions containing halogenated uracil bases that compete X-bonds against classic hydrogen bonds (H-bonds), estimate the enthalpic energies of the competing interactions in the crystal system through crystallographic titrations, and compare the enthalpic and entropic energies of bromine and iodine X-bonds in solution by differential scanning calorimetry. The culmination of these studies demonstrates that enthalpic stabilization of X-bonds increases with increasing polarizability from F to Cl to Br to I, which is consistent with the σ-hole theory of X-bonding. Furthermore, an increase in the X-bonding potential is seen to direct the interaction toward a more ideal geometry. However, the entropic contributions to the total free energies must also be considered to determine how each halogen potentially contributes to the overall stability of the interaction. We find that bromine has the optimal balance between enthalpic and entropic energy components, resulting in the lowest free energy for X-bonding in this DNA system. The X-bond formed by iodine is more enthalpically stable, but this comes with an entropic cost, which we attribute to crowding effects. Thus, the overall free energy of an X-bonding interaction balances the stabilizing electrostatic effects of the σ-hole against the competing effects on the local structural dynamics of the system.

Original languageEnglish (US)
Pages (from-to)4891-4903
Number of pages13
JournalBiochemistry
Volume52
Issue number29
DOIs
StatePublished - Jul 23 2013

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Bromine
Halogens
Entropy
Enthalpy
Iodine
DNA
Chlorine
Free energy
Cruciform DNA
Uracil
Fluorine
Drug Design
Differential Scanning Calorimetry
Static Electricity
Geometry
Structural dynamics
Hydrogen
Biological systems
Titration
Differential scanning calorimetry

ASJC Scopus subject areas

  • Biochemistry

Cite this

Carter, M., Voth, A. R., Scholfield, M. R., Rummel, B., Sowers, L., & Ho, P. S. (2013). Enthalpy-entropy compensation in biomolecular halogen bonds measured in DNA junctions. Biochemistry, 52(29), 4891-4903. https://doi.org/10.1021/bi400590h

Enthalpy-entropy compensation in biomolecular halogen bonds measured in DNA junctions. / Carter, Megan; Voth, Andrea Regier; Scholfield, Matthew R.; Rummel, Brittany; Sowers, Lawrence; Ho, P. Shing.

In: Biochemistry, Vol. 52, No. 29, 23.07.2013, p. 4891-4903.

Research output: Contribution to journalArticle

Carter, M, Voth, AR, Scholfield, MR, Rummel, B, Sowers, L & Ho, PS 2013, 'Enthalpy-entropy compensation in biomolecular halogen bonds measured in DNA junctions', Biochemistry, vol. 52, no. 29, pp. 4891-4903. https://doi.org/10.1021/bi400590h
Carter, Megan ; Voth, Andrea Regier ; Scholfield, Matthew R. ; Rummel, Brittany ; Sowers, Lawrence ; Ho, P. Shing. / Enthalpy-entropy compensation in biomolecular halogen bonds measured in DNA junctions. In: Biochemistry. 2013 ; Vol. 52, No. 29. pp. 4891-4903.
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