Entry of inflammatory cells into the mouse vagina following application of candidate microbicides

Comparison of detergent-based and sulfated polymer-based agents

Gregg Milligan, Kristen L. Dudley, Nigel Bourne, Alisa Reece, Lawrence R. Stanberry

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

Background: Because topical microbicides designed to prevent the spread of sexually transmitted diseases may be applied frequently, it is important to ensure product safety as well as efficacy. A murine model was developed to test for induction of inflammatory responses following application of candidate microbicides. Goal: A comparison was made of the induction of inflammation following vaginal application of detergent-based and sulfated polymer-based microbicides. Study Design: Vaginal leukocytes were collected, identified, and quantified following microbicide application to detect the entry of inflammatory leukocytes into the vaginal lumen. Results: Large numbers of neutrophils and macrophages entered the vaginal lumen following a single application of detergent-based microbicides. No significant increase in vaginal leukocytes was detected following a single or repeated application of sulfated polymer-based microbicides. Conclusion: Application of sulfated polymer-based microbicides was less likely to result in inflammatory responses than was application of detergent-based compounds. This murine model should prove useful as part of a screening process to prioritize candidate microbicides before clinical trial.

Original languageEnglish
Pages (from-to)597-605
Number of pages9
JournalSexually Transmitted Diseases
Volume29
Issue number10
StatePublished - Oct 1 2002

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Vagina
Anti-Infective Agents
Detergents
Polymers
Leukocytes
Local Anti-Infective Agents
Sexually Transmitted Diseases
Neutrophils
Macrophages
Clinical Trials
Inflammation
Safety

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)

Cite this

Entry of inflammatory cells into the mouse vagina following application of candidate microbicides : Comparison of detergent-based and sulfated polymer-based agents. / Milligan, Gregg; Dudley, Kristen L.; Bourne, Nigel; Reece, Alisa; Stanberry, Lawrence R.

In: Sexually Transmitted Diseases, Vol. 29, No. 10, 01.10.2002, p. 597-605.

Research output: Contribution to journalArticle

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