Environmental enrichment alters protein expression as well as the proteomic response to cocaine in rat nucleus accumbens

Cheryl F. Lichti, Xiuzhen Fan, Robert D. English, Yafang Zhang, Dingge Li, Fanping Kong, Mala Sinha, Clark R. Andersen, Heidi Spratt, Bruce A. Luxon, Thomas A. Green

Research output: Contribution to journalArticle

22 Scopus citations

Abstract

Prior research demonstrated that environmental enrichment creates individual differences in behavior leading to a protective addiction phenotype in rats. Understanding the mechanisms underlying this phenotype will guide selection of targets for much-needed novel pharmacotherapeutics. The current study investigates differences in proteome expression in the nucleus accumbens of enriched and isolated rats and the proteomic response to cocaine self-administration using a liquid chromatography mass spectrometry (LCMS) technique to quantify 1917 proteins. Results of complementary Ingenuity Pathways Analyses (IPA) and gene set enrichment analyses (GSEA), both performed using protein quantitative data, demonstrate that cocaine increases vesicular transporters for dopamine and glutamate as well as increasing proteins in the RhoA pathway. Further, cocaine regulates proteins related to ERK, CREB and AKT signaling. Environmental enrichment altered expression of a large number of proteins implicated in a diverse number of neuronal functions (e.g., energy production, mRNA splicing, and ubiquitination), molecular cascades (e.g., protein kinases), psychiatric disorders (e.g., mood disorders), and neurodegenerative diseases (e.g., Huntington's and Alzheimer's diseases). Upregulation of energy metabolism components in EC rats was verified using RNA sequencing. Most of the biological functions and pathways listed above were also identified in the Cocaine X Enrichment interaction analysis, providing clear evidence that enriched and isolated rats respond quite differently to cocaine exposure. The overall impression of the current results is that enriched saline-administering rats have a unique proteomic complement compared to enriched cocaine-administering rats as well as saline and cocaine-taking isolated rats. These results identify possible mechanisms of the protective phenotype and provide fertile soil for developing novel pharmacotherapeutics. Proteomics data are available via ProteomeXchange with identifier PXD000990.

Original languageEnglish (US)
Article number246
JournalFrontiers in Behavioral Neuroscience
Volume8
Issue numberJULY
DOIs
StatePublished - Jul 21 2014

Keywords

  • Cocaine
  • Differential rearing
  • Drug abuse
  • Drug addiction
  • Label-free quantification
  • Proteomics

ASJC Scopus subject areas

  • Neuropsychology and Physiological Psychology
  • Cognitive Neuroscience
  • Behavioral Neuroscience

Fingerprint Dive into the research topics of 'Environmental enrichment alters protein expression as well as the proteomic response to cocaine in rat nucleus accumbens'. Together they form a unique fingerprint.

  • Cite this