Environmental enrichment enhances sensitization to GBR 12935-induced activity and decreases dopamine transporter function in the medial prefrontal cortex

Jun Zhu, Thomas Green, Michael T. Bardo, Linda P. Dwoskin

Research output: Contribution to journalArticle

73 Citations (Scopus)

Abstract

Rats raised in an enriched condition (EC) during development display increased hyperactivity to the effect of acute amphetamine compared to rats raised in an impoverished condition (IC). The present study determined whether environmental enrichment differentially alters the effects of GBR 12935 administration, a selective dopamine transporter (DAT) inhibitor. Acutely, EC rats showed a greater, dose-dependent GBR 12935-induced increase in activity compared to IC rats; however, basal activity for EC rats was lower than for IC rats. After repeated GBR 12935, only EC rats exhibited behavioral sensitization. Kinetic analysis of DAT function in medial prefrontal cortex (mPFC) revealed that the maximal velocity of [3H]dopamine ([3H]DA) uptake in EC rats was less than in IC rats (4.9±0.6 and 7.7±0.6pmol/min/ mg, respectively), but not in striatum or nucleus accumbens. Furthermore, GBR 12935-induced inhibition of DAT function, [3H]GBR 12935 binding density and DA content in mPFC, striatum and nucleus accumbens were not different between EC and IC rats. However, dihydroxyphenylacetic acid content in mPFC was lower in EC than IC rats, whereas no differences were found in striatum and nucleus accumbens. These results suggest that EC-induced changes in activity may be due to decreased DAT function and decreased DA metabolism in the mPFC.

Original languageEnglish (US)
Pages (from-to)107-117
Number of pages11
JournalBehavioural Brain Research
Volume148
Issue number1-2
DOIs
StatePublished - Jan 5 2004
Externally publishedYes

Fingerprint

Dopamine Plasma Membrane Transport Proteins
Prefrontal Cortex
Nucleus Accumbens
GBR 12935
Amphetamine
Dopamine

Keywords

  • Activity
  • Dopamine transporter
  • Dopamine uptake
  • Environmental enrichment
  • GBR 12935
  • Medial prefrontal cortex

ASJC Scopus subject areas

  • Behavioral Neuroscience

Cite this

Environmental enrichment enhances sensitization to GBR 12935-induced activity and decreases dopamine transporter function in the medial prefrontal cortex. / Zhu, Jun; Green, Thomas; Bardo, Michael T.; Dwoskin, Linda P.

In: Behavioural Brain Research, Vol. 148, No. 1-2, 05.01.2004, p. 107-117.

Research output: Contribution to journalArticle

@article{a836d22ba5c84f07af1b68667610897c,
title = "Environmental enrichment enhances sensitization to GBR 12935-induced activity and decreases dopamine transporter function in the medial prefrontal cortex",
abstract = "Rats raised in an enriched condition (EC) during development display increased hyperactivity to the effect of acute amphetamine compared to rats raised in an impoverished condition (IC). The present study determined whether environmental enrichment differentially alters the effects of GBR 12935 administration, a selective dopamine transporter (DAT) inhibitor. Acutely, EC rats showed a greater, dose-dependent GBR 12935-induced increase in activity compared to IC rats; however, basal activity for EC rats was lower than for IC rats. After repeated GBR 12935, only EC rats exhibited behavioral sensitization. Kinetic analysis of DAT function in medial prefrontal cortex (mPFC) revealed that the maximal velocity of [3H]dopamine ([3H]DA) uptake in EC rats was less than in IC rats (4.9±0.6 and 7.7±0.6pmol/min/ mg, respectively), but not in striatum or nucleus accumbens. Furthermore, GBR 12935-induced inhibition of DAT function, [3H]GBR 12935 binding density and DA content in mPFC, striatum and nucleus accumbens were not different between EC and IC rats. However, dihydroxyphenylacetic acid content in mPFC was lower in EC than IC rats, whereas no differences were found in striatum and nucleus accumbens. These results suggest that EC-induced changes in activity may be due to decreased DAT function and decreased DA metabolism in the mPFC.",
keywords = "Activity, Dopamine transporter, Dopamine uptake, Environmental enrichment, GBR 12935, Medial prefrontal cortex",
author = "Jun Zhu and Thomas Green and Bardo, {Michael T.} and Dwoskin, {Linda P.}",
year = "2004",
month = "1",
day = "5",
doi = "10.1016/S0166-4328(03)00190-6",
language = "English (US)",
volume = "148",
pages = "107--117",
journal = "Behavioural Brain Research",
issn = "0166-4328",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Environmental enrichment enhances sensitization to GBR 12935-induced activity and decreases dopamine transporter function in the medial prefrontal cortex

AU - Zhu, Jun

AU - Green, Thomas

AU - Bardo, Michael T.

AU - Dwoskin, Linda P.

PY - 2004/1/5

Y1 - 2004/1/5

N2 - Rats raised in an enriched condition (EC) during development display increased hyperactivity to the effect of acute amphetamine compared to rats raised in an impoverished condition (IC). The present study determined whether environmental enrichment differentially alters the effects of GBR 12935 administration, a selective dopamine transporter (DAT) inhibitor. Acutely, EC rats showed a greater, dose-dependent GBR 12935-induced increase in activity compared to IC rats; however, basal activity for EC rats was lower than for IC rats. After repeated GBR 12935, only EC rats exhibited behavioral sensitization. Kinetic analysis of DAT function in medial prefrontal cortex (mPFC) revealed that the maximal velocity of [3H]dopamine ([3H]DA) uptake in EC rats was less than in IC rats (4.9±0.6 and 7.7±0.6pmol/min/ mg, respectively), but not in striatum or nucleus accumbens. Furthermore, GBR 12935-induced inhibition of DAT function, [3H]GBR 12935 binding density and DA content in mPFC, striatum and nucleus accumbens were not different between EC and IC rats. However, dihydroxyphenylacetic acid content in mPFC was lower in EC than IC rats, whereas no differences were found in striatum and nucleus accumbens. These results suggest that EC-induced changes in activity may be due to decreased DAT function and decreased DA metabolism in the mPFC.

AB - Rats raised in an enriched condition (EC) during development display increased hyperactivity to the effect of acute amphetamine compared to rats raised in an impoverished condition (IC). The present study determined whether environmental enrichment differentially alters the effects of GBR 12935 administration, a selective dopamine transporter (DAT) inhibitor. Acutely, EC rats showed a greater, dose-dependent GBR 12935-induced increase in activity compared to IC rats; however, basal activity for EC rats was lower than for IC rats. After repeated GBR 12935, only EC rats exhibited behavioral sensitization. Kinetic analysis of DAT function in medial prefrontal cortex (mPFC) revealed that the maximal velocity of [3H]dopamine ([3H]DA) uptake in EC rats was less than in IC rats (4.9±0.6 and 7.7±0.6pmol/min/ mg, respectively), but not in striatum or nucleus accumbens. Furthermore, GBR 12935-induced inhibition of DAT function, [3H]GBR 12935 binding density and DA content in mPFC, striatum and nucleus accumbens were not different between EC and IC rats. However, dihydroxyphenylacetic acid content in mPFC was lower in EC than IC rats, whereas no differences were found in striatum and nucleus accumbens. These results suggest that EC-induced changes in activity may be due to decreased DAT function and decreased DA metabolism in the mPFC.

KW - Activity

KW - Dopamine transporter

KW - Dopamine uptake

KW - Environmental enrichment

KW - GBR 12935

KW - Medial prefrontal cortex

UR - http://www.scopus.com/inward/record.url?scp=0346134979&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0346134979&partnerID=8YFLogxK

U2 - 10.1016/S0166-4328(03)00190-6

DO - 10.1016/S0166-4328(03)00190-6

M3 - Article

VL - 148

SP - 107

EP - 117

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

IS - 1-2

ER -