Environmental estrogens induce mast cell degranulation and enhance IgE-mediated release of allergic mediators

Shin Ichiro Narita, Randall M. Goldblum, Cheryl S. Watson, Edward G. Brooks, D. Mark Estes, Edward M. Curran, Terumi Midoro-Horiuti

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Background: Prevalence and morbidity of allergic diseases have increased over the last decades. Based on the recently recognized differences in asthma prevalence between the sexes, we have examined the effect of endogenous estrogens on a key element of the allergic response. Some lipophilic pollutants have estrogen-like activities and are termed environmental estrogens. These pollutants tend to degrade slowly in the environment and to bioaccumulate and bioconcentrate in the food chain; they also have long biological half-lives. Objectives: Our goal in this study was to identify possible pathogenic roles for environmental estrogens in the development of allergic diseases. Methods: We screened a number of environmental estrogens for their ability to modulate the release of allergic mediators from mast cells. We incubated a human mast cell line and primary mast cell cultures derived from bone marrow of wild type and estrogen receptor α (ER-α)-deficient mice with environmental estrogens with and without estradiol or IgE and allergens. We assessed degranulation of mast cells by quantifying the release of β-hexosaminidase. Results: All of the environmental estrogens tested caused rapid, dose-related release of β-hexosaminidase from mast cells and enhanced IgE-mediated release. The combination of physiologic concentrations of 17β-estradiol and several concentrations of environmental estrogens had additive effects on mast cell degranulation. Comparison of bone marrow mast cells from ER-α-sufficient and ER-α-deficient mice indicated that much of the effect of environmental estrogens was mediated by ER-α. Conclusions: Our findings suggest that estrogenic environmental pollutants might promote allergic diseases by inducing and enhancing mast cell degranulation by physiologic estrogens and exposure to allergens.

Original languageEnglish (US)
Pages (from-to)48-52
Number of pages5
JournalEnvironmental Health Perspectives
Volume115
Issue number1
DOIs
StatePublished - Jan 2007

Fingerprint

Cell Degranulation
Mast Cells
Immunoglobulin E
Estrogens
pollutant
bone
Estrogen Receptors
asthma
morbidity
food chain
half life
Hexosaminidases
Allergens
Estradiol
Bone
effect
Environmental Pollutants
Primary Cell Culture
Food Chain
Response Elements

Keywords

  • β-hexosaminidase
  • Allergy
  • Asthma
  • Environmental estrogen
  • Estradiol
  • Estrogen receptor α
  • IgE
  • Mast cells

ASJC Scopus subject areas

  • Environmental Science(all)
  • Environmental Chemistry
  • Public Health, Environmental and Occupational Health

Cite this

Environmental estrogens induce mast cell degranulation and enhance IgE-mediated release of allergic mediators. / Narita, Shin Ichiro; Goldblum, Randall M.; Watson, Cheryl S.; Brooks, Edward G.; Estes, D. Mark; Curran, Edward M.; Midoro-Horiuti, Terumi.

In: Environmental Health Perspectives, Vol. 115, No. 1, 01.2007, p. 48-52.

Research output: Contribution to journalArticle

Narita, Shin Ichiro ; Goldblum, Randall M. ; Watson, Cheryl S. ; Brooks, Edward G. ; Estes, D. Mark ; Curran, Edward M. ; Midoro-Horiuti, Terumi. / Environmental estrogens induce mast cell degranulation and enhance IgE-mediated release of allergic mediators. In: Environmental Health Perspectives. 2007 ; Vol. 115, No. 1. pp. 48-52.
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