Enzymatic and envelope-converting activities of pars recta oviductal fluid from Xenopus laevis

Mary Ann Bakos, Alexander Kurosky, Jerry L. Hedrick

    Research output: Contribution to journalArticle

    27 Citations (Scopus)

    Abstract

    Conversion of the coelomic egg envelope to the vitelline envelope of the Xenopus laevis egg is known to take place in the pars recta (PR) region of the oviduct. A method for collecting fluid generated from PR cultured in vitro was devised which enhanced the recovery of envelope-converting factors. By the criteria of melting temperature analysis, sodium dodecyl sulfate-polyacrylamide gel electrophoresis, 125I labeling, ferritin binding, and in vitro fertilization assays, the secretions collected from PR cultured in vitro were capable of modifying the envelope in a manner analogous to that which occurred in vivo, including the limited hydrolysis of one envelope glycoprotein. Hydrolytic activities present in PR fluid were assayed with a number of peptide and carbohydrate substrates. Enzymes which hydrolyzed t-butyloxycarbonyl-Leu-Ser-Thr-Arg-methylcouma t-butyloxycarbonyl-Phe-Ser-Arg-methylcoumarylamide, and t-butyloxycarbonyl-Val-Leu-Lys-methylcoumarylamide were found to be present in PR fluid at levels elevated by threefold or more over amounts found in a comparable volume of blood plasma.

    Original languageEnglish (US)
    Pages (from-to)169-176
    Number of pages8
    JournalDevelopmental Biology
    Volume138
    Issue number1
    DOIs
    StatePublished - 1990

    Fingerprint

    Xenopus laevis
    Rectum
    Ovum
    Oviducts
    Plasma Volume
    Fertilization in Vitro
    Ferritins
    Sodium Dodecyl Sulfate
    Freezing
    Polyacrylamide Gel Electrophoresis
    Glycoproteins
    Hydrolysis
    Carbohydrates
    Peptides
    Temperature
    Enzymes
    In Vitro Techniques

    ASJC Scopus subject areas

    • Developmental Biology

    Cite this

    Enzymatic and envelope-converting activities of pars recta oviductal fluid from Xenopus laevis. / Bakos, Mary Ann; Kurosky, Alexander; Hedrick, Jerry L.

    In: Developmental Biology, Vol. 138, No. 1, 1990, p. 169-176.

    Research output: Contribution to journalArticle

    Bakos, Mary Ann ; Kurosky, Alexander ; Hedrick, Jerry L. / Enzymatic and envelope-converting activities of pars recta oviductal fluid from Xenopus laevis. In: Developmental Biology. 1990 ; Vol. 138, No. 1. pp. 169-176.
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