Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates

Thomas Ksiazek, P. E. Rollin, P. B. Jahrling, E. Johnson, D. W. Dalgard, C. J. Peters

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

A sandwich enzyme immunosorbent assay (EIA) using a mixture of mouse monoclonal antibodies for antigen capture and polyclonal hyperimmune rabbit anti-Ebola virus serum for antigen detection was developed and evaluated on the tissues of monkeys naturally or experimentally infected with strains of Ebola viruses. When compared with virus isolation, the antigen detection EIA was both sensitive and specific: 44 of 45 (97.7%) liver homogenates and 38 of 41 (92.7%) spleen homogenates that were culture positive and tested by both techniques were positive for viral antigen, while 85 of 87 (97.7%) culture- negative liver homogenates and 66 of 66 culture-negative spleen homogenates were found to be antigen negative. The assay, initially developed to detect antigens of prototype African strains of Ebola virus, reliably detected related strains of Ebola virus found during two recent outbreaks of Ebola virus infection among imported, quarantined Macaca fascicularis monkeys in the United States. The assay allows economical and rapid testing of large numbers of tissue specimens. Antigen was found in homogenates of spleen and liver and in serum.

Original languageEnglish (US)
Pages (from-to)947-950
Number of pages4
JournalJournal of Clinical Microbiology
Volume30
Issue number4
StatePublished - 1992
Externally publishedYes

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Ebolavirus
Immunosorbents
Enzyme Assays
Primates
Antigens
Spleen
Haplorhini
Liver
Ebola Hemorrhagic Fever
Macaca fascicularis
Viral Antigens
Serum
Disease Outbreaks
Monoclonal Antibodies
Rabbits
Viruses

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Ksiazek, T., Rollin, P. E., Jahrling, P. B., Johnson, E., Dalgard, D. W., & Peters, C. J. (1992). Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates. Journal of Clinical Microbiology, 30(4), 947-950.

Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates. / Ksiazek, Thomas; Rollin, P. E.; Jahrling, P. B.; Johnson, E.; Dalgard, D. W.; Peters, C. J.

In: Journal of Clinical Microbiology, Vol. 30, No. 4, 1992, p. 947-950.

Research output: Contribution to journalArticle

Ksiazek, T, Rollin, PE, Jahrling, PB, Johnson, E, Dalgard, DW & Peters, CJ 1992, 'Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates', Journal of Clinical Microbiology, vol. 30, no. 4, pp. 947-950.
Ksiazek T, Rollin PE, Jahrling PB, Johnson E, Dalgard DW, Peters CJ. Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates. Journal of Clinical Microbiology. 1992;30(4):947-950.
Ksiazek, Thomas ; Rollin, P. E. ; Jahrling, P. B. ; Johnson, E. ; Dalgard, D. W. ; Peters, C. J. / Enzyme immunosorbent assay for Ebola virus antigens in tissues of infected primates. In: Journal of Clinical Microbiology. 1992 ; Vol. 30, No. 4. pp. 947-950.
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