Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

J. Andrew Grant, Randall M. Goldblum, Richard Rahr, David O. Thueson, Jafar Farnam, James Gillaspy

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

Original languageEnglish (US)
Pages (from-to)112-118
Number of pages7
JournalThe Journal of Allergy and Clinical Immunology
Volume68
Issue number2
DOIs
StatePublished - 1981
Externally publishedYes

Fingerprint

Venoms
Insects
Immunoglobulin G
Enzyme-Linked Immunosorbent Assay
Bee Venoms
Antibodies
Immunotherapy
Wasp Venoms
Insect Bites and Stings
Blocking Antibodies
Basophils
Phospholipases A
Histamine Release
Radioimmunoassay
Therapeutics

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy

Cite this

Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms. / Grant, J. Andrew; Goldblum, Randall M.; Rahr, Richard; Thueson, David O.; Farnam, Jafar; Gillaspy, James.

In: The Journal of Allergy and Clinical Immunology, Vol. 68, No. 2, 1981, p. 112-118.

Research output: Contribution to journalArticle

Grant, J. Andrew ; Goldblum, Randall M. ; Rahr, Richard ; Thueson, David O. ; Farnam, Jafar ; Gillaspy, James. / Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms. In: The Journal of Allergy and Clinical Immunology. 1981 ; Vol. 68, No. 2. pp. 112-118.
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