Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

J. Andrew Grant, Randall M. Goldblum, Richard Rahr, David O. Thueson, Jafar Farnam, James Gillaspy

Research output: Contribution to journalArticlepeer-review

29 Scopus citations

Abstract

IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

Original languageEnglish (US)
Pages (from-to)112-118
Number of pages7
JournalThe Journal of Allergy and Clinical Immunology
Volume68
Issue number2
DOIs
StatePublished - Aug 1981

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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