Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

J. Andrew Grant; Randall M. Goldblum; Richard Rahr; David O. Thueson; Jafar Farnam; James Gillaspy

doi:10.1016/0091-6749(81)90168-8

Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

J. Andrew Grant
, Randall M. Goldblum
, Richard Rahr
, David O. Thueson
, Jafar Farnam
, James Gillaspy

Research output: Contribution to journal › Article › peer-review

29 Scopus citations

Abstract

IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

Original language	English (US)
Pages (from-to)	112-118
Number of pages	7
Journal	The Journal of Allergy and Clinical Immunology
Volume	68
Issue number	2
DOIs	https://doi.org/10.1016/0091-6749(81)90168-8
State	Published - Aug 1981
Externally published	Yes

ASJC Scopus subject areas

Immunology and Allergy
Immunology

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10.1016/0091-6749(81)90168-8

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@article{51525e72d35a4522829ff25751e092df,

title = "Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms",

abstract = "IgG {"}blocking{"} antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.",

author = "Grant, \{J. Andrew\} and Goldblum, \{Randall M.\} and Richard Rahr and Thueson, \{David O.\} and Jafar Farnam and James Gillaspy",

note = "Funding Information: Supported in part by United States Public Health Service research grant AI-12621, General Clinical Research Program grant RR-73, Allergic Diseases Academic Award AI-00110 (Dr. Grant), Young Investigator Award AI-14879 (Dr. Thueson), the James W. McLaughlin Fellowship Fund (Dr. Famam), and Pharmacia Laboratories, Piscataway, N.J.",

year = "1981",

month = aug,

doi = "10.1016/0091-6749(81)90168-8",

language = "English (US)",

volume = "68",

pages = "112--118",

journal = "The Journal of Allergy and Clinical Immunology",

issn = "0091-6749",

publisher = "Elsevier Inc.",

number = "2",

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TY - JOUR

T1 - Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

AU - Grant, J. Andrew

AU - Goldblum, Randall M.

AU - Rahr, Richard

AU - Thueson, David O.

AU - Farnam, Jafar

AU - Gillaspy, James

N1 - Funding Information: Supported in part by United States Public Health Service research grant AI-12621, General Clinical Research Program grant RR-73, Allergic Diseases Academic Award AI-00110 (Dr. Grant), Young Investigator Award AI-14879 (Dr. Thueson), the James W. McLaughlin Fellowship Fund (Dr. Famam), and Pharmacia Laboratories, Piscataway, N.J.

PY - 1981/8

Y1 - 1981/8

N2 - IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

AB - IgG "blocking" antibodies were measured in patients receiving insect venom immunotherapy. The enzyme-linked immunosorbent assay (ELISA) described herein was found to be sensitive and reproducible. Results with ELISA correlated well with values obtained with a radioimmunoassay and with inhibition of the release of histamine from sensitive basophils. Also, specific antibody titers against phospholipase A and whole bee venom were correlated. Serial determinations of venom-specific IgG antibodies were made in 17 patients receiving Polistes wasp or bee venom immunotherapy. The majority of patients showed a rise in IgG antibodies, which peaked after administration of approximately 500 μg of venom. Only one out of 13 of these venom-treated patients had allergic symptoms after an insect sting while on maintenance therapy.

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UR - https://www.scopus.com/pages/publications/0019395658#tab=citedBy

U2 - 10.1016/0091-6749(81)90168-8

DO - 10.1016/0091-6749(81)90168-8

M3 - Article

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AN - SCOPUS:0019395658

SN - 0091-6749

VL - 68

SP - 112

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JO - The Journal of Allergy and Clinical Immunology

JF - The Journal of Allergy and Clinical Immunology

IS - 2

ER -

Enzyme-linked immunosorbent assay (ELISA) for immunoglobulin G antibodies against insect venoms

Abstract

ASJC Scopus subject areas

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