Enzymes of glutamine metabolism in testa-pericarp and endosperm of developing wheat grain

Nisha Garg, Randhir Singh, Vijay I.P. Batra

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Glutamate dehydrogenase, glutamine synthetase, glutamate synthase, glutamate puruvate transaminase and glutamate oxaloacetate transaminase have been assayed in developing testa-pericarp and endosperm of two wheat varieties, namely Shera (11.6% protein) and C-306 (9.8% protein). On per organ basis, activities of all the enzymes studied, except glutamine synthetase, increased during development. Glutamine synthetase activity decreased during development in the testa-pericarp, whereas, no glutamine synthetase activity could be detected in endosperm of either variety at any stage of development. Compared to testa-pericarp, endosperm had higher activities of glutamate synthase and glutamate pyruvate transaminase. On the whole, enzyme activities in Shera were higher, as compared to C-306. Developmental patterns and relative levels of enzyme activities in the two varieties were more or less the same, when expressed on dry weight basis or as specific activities. The results suggest that ammonia assimilation in developing wheat grain takes place by the glutamate dehydrogenase pathway in the endosperm; and both by the glutamate dehydrogenase and glutamine synthetase-glutamate synthase pathways in the testa-pericarp.

Original languageEnglish (US)
Pages (from-to)1663-1666
Number of pages4
JournalPhytochemistry
Volume24
Issue number8
DOIs
StatePublished - 1985
Externally publishedYes

Keywords

  • Gramineae
  • Triticum aestivum
  • development
  • endosperm
  • glutamate dehydrogenase
  • glutamate oxaloacetate transaminase
  • glutamate pyruvate transaminase.
  • glutamate synthase
  • glutamine synthetase
  • testa-pericarp
  • wheat

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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