Eosinophil recruitment is associated with IL-5, but not with RANTES, twenty-four hours after allergen challenge

Sanjiv Sur, Hirohito Kita, Gerald J. Gleich, Thomas C. Chenier, Loren W. Hunt

Research output: Contribution to journalArticle

58 Citations (Scopus)

Abstract

Several lines of evidence suggest that the chemokine RANTES may play a role in eosinophilia observed during allergic inflammation. To test this hypothesis, six patients with allergic asthma were studied. After performing bronchoalveolar lavage in a lung segment (baseline), segmental bronchoprovocation was performed with saline solution in another segment and with ragweed in a third segment. Bronchoalveolar lavage was performed 24 hours later in the saline-challenged (sham) and ragweed-challenged lung segments. The bronchoalveolar lavage fluids from the baseline, sham, and ragweed segments were analyzed for cell counts and for the levels of IL-5, RANTES, and eosinophil-derived neurotoxin IL-5 levels were elevated in the ragweed (984 ± 588 pg/ml) compared with sham segments (2.8 ± 0.2 pg/ml, p = 0.02). Likewise, RANTES levels were elevated in the ragweed (12.93 ± 3.4 pg/ml) compared with the sham segments (3.05 ± 1.19 pg/ml, p = 0.006). The IL-5 levels correlated with both eosinophil numbers (r = 0.90, p < 0.02) and eosinophil-derived neurotoxin levels (r = 0.89) p < 0.02). In contrast, RANTES levels did not correlate with either eosinophil numbers or eosinophil- derived neurotoxin levels. These results indicate that although both IL-5 and RANTES are elevated 24 hours after allergen challenge, only IL-5 correlates with eosinophil recruitment and degranulation.

Original languageEnglish (US)
Pages (from-to)1272-1278
Number of pages7
JournalJournal of Allergy and Clinical Immunology
Volume97
Issue number6
DOIs
StatePublished - 1996
Externally publishedYes

Fingerprint

Ambrosia
Chemokine CCL5
Interleukin-5
Eosinophils
Eosinophil-Derived Neurotoxin
Allergens
Bronchoalveolar Lavage
Lung
Bronchoalveolar Lavage Fluid
Eosinophilia
Chemokines
Sodium Chloride
Asthma
Cell Count
Inflammation

Keywords

  • asthma
  • chemokines
  • cytokines
  • EDN
  • eosinophils
  • IL-5
  • late phase reaction
  • RANTES

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Eosinophil recruitment is associated with IL-5, but not with RANTES, twenty-four hours after allergen challenge. / Sur, Sanjiv; Kita, Hirohito; Gleich, Gerald J.; Chenier, Thomas C.; Hunt, Loren W.

In: Journal of Allergy and Clinical Immunology, Vol. 97, No. 6, 1996, p. 1272-1278.

Research output: Contribution to journalArticle

Sur, Sanjiv ; Kita, Hirohito ; Gleich, Gerald J. ; Chenier, Thomas C. ; Hunt, Loren W. / Eosinophil recruitment is associated with IL-5, but not with RANTES, twenty-four hours after allergen challenge. In: Journal of Allergy and Clinical Immunology. 1996 ; Vol. 97, No. 6. pp. 1272-1278.
@article{68cdf91209a94e4888f3812a20d85fc1,
title = "Eosinophil recruitment is associated with IL-5, but not with RANTES, twenty-four hours after allergen challenge",
abstract = "Several lines of evidence suggest that the chemokine RANTES may play a role in eosinophilia observed during allergic inflammation. To test this hypothesis, six patients with allergic asthma were studied. After performing bronchoalveolar lavage in a lung segment (baseline), segmental bronchoprovocation was performed with saline solution in another segment and with ragweed in a third segment. Bronchoalveolar lavage was performed 24 hours later in the saline-challenged (sham) and ragweed-challenged lung segments. The bronchoalveolar lavage fluids from the baseline, sham, and ragweed segments were analyzed for cell counts and for the levels of IL-5, RANTES, and eosinophil-derived neurotoxin IL-5 levels were elevated in the ragweed (984 ± 588 pg/ml) compared with sham segments (2.8 ± 0.2 pg/ml, p = 0.02). Likewise, RANTES levels were elevated in the ragweed (12.93 ± 3.4 pg/ml) compared with the sham segments (3.05 ± 1.19 pg/ml, p = 0.006). The IL-5 levels correlated with both eosinophil numbers (r = 0.90, p < 0.02) and eosinophil-derived neurotoxin levels (r = 0.89) p < 0.02). In contrast, RANTES levels did not correlate with either eosinophil numbers or eosinophil- derived neurotoxin levels. These results indicate that although both IL-5 and RANTES are elevated 24 hours after allergen challenge, only IL-5 correlates with eosinophil recruitment and degranulation.",
keywords = "asthma, chemokines, cytokines, EDN, eosinophils, IL-5, late phase reaction, RANTES",
author = "Sanjiv Sur and Hirohito Kita and Gleich, {Gerald J.} and Chenier, {Thomas C.} and Hunt, {Loren W.}",
year = "1996",
doi = "10.1016/S0091-6749(96)70195-1",
language = "English (US)",
volume = "97",
pages = "1272--1278",
journal = "Journal of Allergy and Clinical Immunology",
issn = "0091-6749",
publisher = "Mosby Inc.",
number = "6",

}

TY - JOUR

T1 - Eosinophil recruitment is associated with IL-5, but not with RANTES, twenty-four hours after allergen challenge

AU - Sur, Sanjiv

AU - Kita, Hirohito

AU - Gleich, Gerald J.

AU - Chenier, Thomas C.

AU - Hunt, Loren W.

PY - 1996

Y1 - 1996

N2 - Several lines of evidence suggest that the chemokine RANTES may play a role in eosinophilia observed during allergic inflammation. To test this hypothesis, six patients with allergic asthma were studied. After performing bronchoalveolar lavage in a lung segment (baseline), segmental bronchoprovocation was performed with saline solution in another segment and with ragweed in a third segment. Bronchoalveolar lavage was performed 24 hours later in the saline-challenged (sham) and ragweed-challenged lung segments. The bronchoalveolar lavage fluids from the baseline, sham, and ragweed segments were analyzed for cell counts and for the levels of IL-5, RANTES, and eosinophil-derived neurotoxin IL-5 levels were elevated in the ragweed (984 ± 588 pg/ml) compared with sham segments (2.8 ± 0.2 pg/ml, p = 0.02). Likewise, RANTES levels were elevated in the ragweed (12.93 ± 3.4 pg/ml) compared with the sham segments (3.05 ± 1.19 pg/ml, p = 0.006). The IL-5 levels correlated with both eosinophil numbers (r = 0.90, p < 0.02) and eosinophil-derived neurotoxin levels (r = 0.89) p < 0.02). In contrast, RANTES levels did not correlate with either eosinophil numbers or eosinophil- derived neurotoxin levels. These results indicate that although both IL-5 and RANTES are elevated 24 hours after allergen challenge, only IL-5 correlates with eosinophil recruitment and degranulation.

AB - Several lines of evidence suggest that the chemokine RANTES may play a role in eosinophilia observed during allergic inflammation. To test this hypothesis, six patients with allergic asthma were studied. After performing bronchoalveolar lavage in a lung segment (baseline), segmental bronchoprovocation was performed with saline solution in another segment and with ragweed in a third segment. Bronchoalveolar lavage was performed 24 hours later in the saline-challenged (sham) and ragweed-challenged lung segments. The bronchoalveolar lavage fluids from the baseline, sham, and ragweed segments were analyzed for cell counts and for the levels of IL-5, RANTES, and eosinophil-derived neurotoxin IL-5 levels were elevated in the ragweed (984 ± 588 pg/ml) compared with sham segments (2.8 ± 0.2 pg/ml, p = 0.02). Likewise, RANTES levels were elevated in the ragweed (12.93 ± 3.4 pg/ml) compared with the sham segments (3.05 ± 1.19 pg/ml, p = 0.006). The IL-5 levels correlated with both eosinophil numbers (r = 0.90, p < 0.02) and eosinophil-derived neurotoxin levels (r = 0.89) p < 0.02). In contrast, RANTES levels did not correlate with either eosinophil numbers or eosinophil- derived neurotoxin levels. These results indicate that although both IL-5 and RANTES are elevated 24 hours after allergen challenge, only IL-5 correlates with eosinophil recruitment and degranulation.

KW - asthma

KW - chemokines

KW - cytokines

KW - EDN

KW - eosinophils

KW - IL-5

KW - late phase reaction

KW - RANTES

UR - http://www.scopus.com/inward/record.url?scp=0029984040&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0029984040&partnerID=8YFLogxK

U2 - 10.1016/S0091-6749(96)70195-1

DO - 10.1016/S0091-6749(96)70195-1

M3 - Article

C2 - 8648023

AN - SCOPUS:0029984040

VL - 97

SP - 1272

EP - 1278

JO - Journal of Allergy and Clinical Immunology

JF - Journal of Allergy and Clinical Immunology

SN - 0091-6749

IS - 6

ER -