Epidemiology and HBGA-susceptibility investigation of a G9P[8] rotavirus outbreak in a school in Lechang, China

Lun Ai Guo, Meng Zhang, Yu zhen Hou, Haitao Hu, Ling Fang, Ming Tan, Qiong Huang, Hui Li, Li Mei Sun, Xi Jiang, Wen Fang Tan, Shixing Tang, Xu Fu Zhang, Ying Chun Dai

Research output: Contribution to journalArticlepeer-review

Abstract

Rotaviruses cause severe gastroenteritis in infants, in which the viruses interact with human histo-blood group antigens (HBGAs) as attachment and host susceptibility factors. While gastroenteritis outbreaks caused by rotaviruses are uncommon in adolescents, we reported here one that occurred in a middle school in China. Rectal swabs and saliva samples were collected from symptomatic and asymptomatic students, and samples were also collected from the environment. Using PCR, followed by DNA sequencing, a single G9P[8] rotavirus strain was identified as the causative agent. The attack rate of the outbreak was 13.5% for boarders, which was significantly higher than that of day students (1.8%). Person-to-person transmission was the most plausible transmission mode. The HBGA phenotypes of the individuals in the study were determined by enzyme immunoassay, using saliva samples, while recombinant VP8* protein of the causative rotavirus strain was produced for HBGA binding assays to evaluate the host susceptibility. Our data showed that secretor individuals had a significantly higher risk of infection than nonsecretors. Accordingly, the VP8* protein bound nearly all secretor saliva samples, but not those of nonsecretors, explaining the observed infection of secretor individuals only. This is the first single-outbreak-based investigation showing that P[8] rotavirus infected only secretors. Our investigation also suggests that health education of school students is an important countermeasure against an outbreak of communicable disease.

Original languageEnglish (US)
Pages (from-to)1311-1320
Number of pages10
JournalArchives of virology
Volume165
Issue number6
DOIs
StatePublished - Jun 1 2020

ASJC Scopus subject areas

  • Virology

Fingerprint Dive into the research topics of 'Epidemiology and HBGA-susceptibility investigation of a G9P[8] rotavirus outbreak in a school in Lechang, China'. Together they form a unique fingerprint.

Cite this