Epigenetic silencing of the non-coding RNA nc886 provokes oncogenes during human esophageal tumorigenesis

Hyun Sung Lee, Kwanbok Lee, Hee Jin Jang, Geon Kook Lee, Jong Lyul Park, Seon Young Kim, Sang Bae Kim, Betty H. Johnson, Jae Ill Zo, Ju Seog Lee, Yong Sun Lee

    Research output: Contribution to journalArticle

    28 Citations (Scopus)

    Abstract

    nc886 (= vtRNA2-1 or pre-miR-886) is a recently discovered noncoding RNA that is a cellular PKR (Protein Kinase RNA-activated) ligand and repressor. nc886 has been suggested to be a tumor suppressor, solely based on its expression pattern and genomic locus. In this report, we have provided sufficient evidence that nc886 is a putative tumor suppressor in esophageal squamous cell carcinoma (ESCC). In 84 paired specimens from ESCC patients, nc886 expression is significantly lower in tumors than their normal adjacent tissues. More importantly, decreased expression of nc886 is significantly associated with shorter recurrence-free survival of the patients. Suppression of nc886 is mediated by CpG hypermethylation of its promoter, as evidenced by its significant negative correlation to nc886 expression in ESCC tumors and by induced expression of nc886 upon demethylation of its promoter. Knockdown of nc886 and consequent PKR activation induce FOS and MYC oncogenes as well as some inflammatory genes including oncogenic NF-κB. When ectopically expressed, nc886 inhibits proliferation of ESCC cells, further demonstrating that nc886 could be a tumor suppressor. All these findings implicate nc886 as a novel, putative tumor suppressor that is epigenetically silenced and regulates the expression of oncogenes in ESCC.

    Original languageEnglish (US)
    Pages (from-to)3472-3481
    Number of pages10
    JournalOncotarget
    Volume5
    Issue number11
    StatePublished - 2014

    Fingerprint

    Untranslated RNA
    Oncogenes
    Epigenomics
    Carcinogenesis
    eIF-2 Kinase
    Neoplasms
    Esophageal Squamous Cell Carcinoma
    Ligands
    Recurrence
    Survival
    Genes

    Keywords

    • CpG DNA methylation
    • ESCC
    • nc886
    • Tumor suppressor

    ASJC Scopus subject areas

    • Oncology

    Cite this

    Lee, H. S., Lee, K., Jang, H. J., Lee, G. K., Park, J. L., Kim, S. Y., ... Lee, Y. S. (2014). Epigenetic silencing of the non-coding RNA nc886 provokes oncogenes during human esophageal tumorigenesis. Oncotarget, 5(11), 3472-3481.

    Epigenetic silencing of the non-coding RNA nc886 provokes oncogenes during human esophageal tumorigenesis. / Lee, Hyun Sung; Lee, Kwanbok; Jang, Hee Jin; Lee, Geon Kook; Park, Jong Lyul; Kim, Seon Young; Kim, Sang Bae; Johnson, Betty H.; Zo, Jae Ill; Lee, Ju Seog; Lee, Yong Sun.

    In: Oncotarget, Vol. 5, No. 11, 2014, p. 3472-3481.

    Research output: Contribution to journalArticle

    Lee, HS, Lee, K, Jang, HJ, Lee, GK, Park, JL, Kim, SY, Kim, SB, Johnson, BH, Zo, JI, Lee, JS & Lee, YS 2014, 'Epigenetic silencing of the non-coding RNA nc886 provokes oncogenes during human esophageal tumorigenesis', Oncotarget, vol. 5, no. 11, pp. 3472-3481.
    Lee HS, Lee K, Jang HJ, Lee GK, Park JL, Kim SY et al. Epigenetic silencing of the non-coding RNA nc886 provokes oncogenes during human esophageal tumorigenesis. Oncotarget. 2014;5(11):3472-3481.
    Lee, Hyun Sung ; Lee, Kwanbok ; Jang, Hee Jin ; Lee, Geon Kook ; Park, Jong Lyul ; Kim, Seon Young ; Kim, Sang Bae ; Johnson, Betty H. ; Zo, Jae Ill ; Lee, Ju Seog ; Lee, Yong Sun. / Epigenetic silencing of the non-coding RNA nc886 provokes oncogenes during human esophageal tumorigenesis. In: Oncotarget. 2014 ; Vol. 5, No. 11. pp. 3472-3481.
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