Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis

Ryan S. Dhindsa, Shelton Bradrick, Xiaodi Yao, Erin L. Heinzen, Slave Petrovski, Brian J. Krueger, Michael R. Johnson, Wayne N. Frankel, Steven Petrou, Rebecca M. Boumil, David B. Goldstein

Research output: Contribution to journalArticle

22 Citations (Scopus)

Abstract

Objective: To elucidate the functional consequences of epileptic encephalopathy-causing de novo mutations in DNM1 (A177P, K206N, G359A), which encodes a large mechanochemical GTPase essential for neuronal synaptic vesicle endocytosis. Methods: HeLa and COS-7 cells transfected with wild-type and mutant DNM1 constructs were used for transferrin assays, high-content imaging, colocalization studies, Western blotting, and electron microscopy (EM). EM was also conducted on the brain sections of mice harboring a middle-domain Dnm1 mutation (Dnm1Ftfl). Results: We demonstrate that the expression of each mutant protein decreased endocytosis activity in a dominant-negative manner. One of the G-domain mutations, K206N, decreased protein levels. The G359A mutation, which occurs in the middle domain, disrupted higher-order DNM1 oligomerization. EM of mutant DNM1-transfected HeLa cells and of the Dnm1Ftfl mouse brain revealed vesicle defects, indicating that the mutations likely interfere with DNM1's vesicle scission activity. Conclusion: Together, these data suggest that the dysfunction of vesicle scission during synaptic vesicle endocytosis can lead to serious early-onset epilepsies.

Original languageEnglish (US)
Article numbere4
JournalNeurology: Genetics
Volume1
Issue number1
DOIs
StatePublished - Jun 1 2015
Externally publishedYes

Fingerprint

Synaptic Vesicles
Brain Diseases
Endocytosis
Mutation
Electron Microscopy
COS Cells
GTP Phosphohydrolases
Brain
Mutant Proteins
Transferrin
HeLa Cells
Epilepsy
Western Blotting
Proteins

ASJC Scopus subject areas

  • Clinical Neurology
  • Genetics(clinical)

Cite this

Dhindsa, R. S., Bradrick, S., Yao, X., Heinzen, E. L., Petrovski, S., Krueger, B. J., ... Goldstein, D. B. (2015). Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis. Neurology: Genetics, 1(1), [e4]. https://doi.org/10.1212/01.NXG.0000464295.65736.da

Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis. / Dhindsa, Ryan S.; Bradrick, Shelton; Yao, Xiaodi; Heinzen, Erin L.; Petrovski, Slave; Krueger, Brian J.; Johnson, Michael R.; Frankel, Wayne N.; Petrou, Steven; Boumil, Rebecca M.; Goldstein, David B.

In: Neurology: Genetics, Vol. 1, No. 1, e4, 01.06.2015.

Research output: Contribution to journalArticle

Dhindsa, RS, Bradrick, S, Yao, X, Heinzen, EL, Petrovski, S, Krueger, BJ, Johnson, MR, Frankel, WN, Petrou, S, Boumil, RM & Goldstein, DB 2015, 'Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis', Neurology: Genetics, vol. 1, no. 1, e4. https://doi.org/10.1212/01.NXG.0000464295.65736.da
Dhindsa, Ryan S. ; Bradrick, Shelton ; Yao, Xiaodi ; Heinzen, Erin L. ; Petrovski, Slave ; Krueger, Brian J. ; Johnson, Michael R. ; Frankel, Wayne N. ; Petrou, Steven ; Boumil, Rebecca M. ; Goldstein, David B. / Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis. In: Neurology: Genetics. 2015 ; Vol. 1, No. 1.
@article{7e7464a9fd3a4792a35c6f7f4f2814d3,
title = "Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis",
abstract = "Objective: To elucidate the functional consequences of epileptic encephalopathy-causing de novo mutations in DNM1 (A177P, K206N, G359A), which encodes a large mechanochemical GTPase essential for neuronal synaptic vesicle endocytosis. Methods: HeLa and COS-7 cells transfected with wild-type and mutant DNM1 constructs were used for transferrin assays, high-content imaging, colocalization studies, Western blotting, and electron microscopy (EM). EM was also conducted on the brain sections of mice harboring a middle-domain Dnm1 mutation (Dnm1Ftfl). Results: We demonstrate that the expression of each mutant protein decreased endocytosis activity in a dominant-negative manner. One of the G-domain mutations, K206N, decreased protein levels. The G359A mutation, which occurs in the middle domain, disrupted higher-order DNM1 oligomerization. EM of mutant DNM1-transfected HeLa cells and of the Dnm1Ftfl mouse brain revealed vesicle defects, indicating that the mutations likely interfere with DNM1's vesicle scission activity. Conclusion: Together, these data suggest that the dysfunction of vesicle scission during synaptic vesicle endocytosis can lead to serious early-onset epilepsies.",
author = "Dhindsa, {Ryan S.} and Shelton Bradrick and Xiaodi Yao and Heinzen, {Erin L.} and Slave Petrovski and Krueger, {Brian J.} and Johnson, {Michael R.} and Frankel, {Wayne N.} and Steven Petrou and Boumil, {Rebecca M.} and Goldstein, {David B.}",
year = "2015",
month = "6",
day = "1",
doi = "10.1212/01.NXG.0000464295.65736.da",
language = "English (US)",
volume = "1",
journal = "Neurology: Genetics",
issn = "2376-7839",
publisher = "Lippincott Williams and Wilkins",
number = "1",

}

TY - JOUR

T1 - Epileptic encephalopathy-causing mutations in DNM1 impair synaptic vesicle endocytosis

AU - Dhindsa, Ryan S.

AU - Bradrick, Shelton

AU - Yao, Xiaodi

AU - Heinzen, Erin L.

AU - Petrovski, Slave

AU - Krueger, Brian J.

AU - Johnson, Michael R.

AU - Frankel, Wayne N.

AU - Petrou, Steven

AU - Boumil, Rebecca M.

AU - Goldstein, David B.

PY - 2015/6/1

Y1 - 2015/6/1

N2 - Objective: To elucidate the functional consequences of epileptic encephalopathy-causing de novo mutations in DNM1 (A177P, K206N, G359A), which encodes a large mechanochemical GTPase essential for neuronal synaptic vesicle endocytosis. Methods: HeLa and COS-7 cells transfected with wild-type and mutant DNM1 constructs were used for transferrin assays, high-content imaging, colocalization studies, Western blotting, and electron microscopy (EM). EM was also conducted on the brain sections of mice harboring a middle-domain Dnm1 mutation (Dnm1Ftfl). Results: We demonstrate that the expression of each mutant protein decreased endocytosis activity in a dominant-negative manner. One of the G-domain mutations, K206N, decreased protein levels. The G359A mutation, which occurs in the middle domain, disrupted higher-order DNM1 oligomerization. EM of mutant DNM1-transfected HeLa cells and of the Dnm1Ftfl mouse brain revealed vesicle defects, indicating that the mutations likely interfere with DNM1's vesicle scission activity. Conclusion: Together, these data suggest that the dysfunction of vesicle scission during synaptic vesicle endocytosis can lead to serious early-onset epilepsies.

AB - Objective: To elucidate the functional consequences of epileptic encephalopathy-causing de novo mutations in DNM1 (A177P, K206N, G359A), which encodes a large mechanochemical GTPase essential for neuronal synaptic vesicle endocytosis. Methods: HeLa and COS-7 cells transfected with wild-type and mutant DNM1 constructs were used for transferrin assays, high-content imaging, colocalization studies, Western blotting, and electron microscopy (EM). EM was also conducted on the brain sections of mice harboring a middle-domain Dnm1 mutation (Dnm1Ftfl). Results: We demonstrate that the expression of each mutant protein decreased endocytosis activity in a dominant-negative manner. One of the G-domain mutations, K206N, decreased protein levels. The G359A mutation, which occurs in the middle domain, disrupted higher-order DNM1 oligomerization. EM of mutant DNM1-transfected HeLa cells and of the Dnm1Ftfl mouse brain revealed vesicle defects, indicating that the mutations likely interfere with DNM1's vesicle scission activity. Conclusion: Together, these data suggest that the dysfunction of vesicle scission during synaptic vesicle endocytosis can lead to serious early-onset epilepsies.

UR - http://www.scopus.com/inward/record.url?scp=85046981775&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85046981775&partnerID=8YFLogxK

U2 - 10.1212/01.NXG.0000464295.65736.da

DO - 10.1212/01.NXG.0000464295.65736.da

M3 - Article

VL - 1

JO - Neurology: Genetics

JF - Neurology: Genetics

SN - 2376-7839

IS - 1

M1 - e4

ER -