Epitope mapping with membrane-bound synthetic overlapping peptides

Terumi Midoro-Horiuti, Randall M. Goldblum

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Epitope mapping with synthetic overlapping peptides is used for identifying epitopes of monoclonal antibodies (mAbs) and antibodies from patient sera (Midoro-Horiuti et al. Mol Immunol 43:509-518, 2006; Ivanciuc et al. J Agric Food Chem 51:4830-4837, 2003; Midoro-Horiuti et al. Mol Immunol 40:555-562, 2003; Wang et al. J Allergy Clin Immunol 125:695-702, 702.e1-702.e6, 2010). When the mAbs recognize epitopes that are also recognized by patients of interest, they may be useful as surrogates for patient antibodies.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages421-426
Number of pages6
Volume1131
ISBN (Print)9781627039918
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1131
ISSN (Print)10643745

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Keywords

  • Antigen
  • Epitope
  • Monoclonal antibody
  • Overlapping peptides
  • Patient serum

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Midoro-Horiuti, T., & Goldblum, R. M. (2014). Epitope mapping with membrane-bound synthetic overlapping peptides. In Methods in Molecular Biology (Vol. 1131, pp. 421-426). (Methods in Molecular Biology; Vol. 1131). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-992-5_25