Epitope mapping with random phage display library

Terumi Midoro-Horiuti, Randall M. Goldblum

Research output: Chapter in Book/Report/Conference proceedingChapter

Abstract

Random phage display library is used to map conformational as well as linear epitopes. These libraries are available in varying lengths and with circularization. We provide here a protocol conveying our experience using a commercially available peptide phage display library, which in our hands provides good results.

Original languageEnglish (US)
Title of host publicationMethods in Molecular Biology
PublisherHumana Press Inc.
Pages477-484
Number of pages8
Volume1131
ISBN (Print)9781627039918
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1131
ISSN (Print)10643745

    Fingerprint

Keywords

  • Allergen
  • Conformational epitope
  • Monoclonal antibody
  • Random phage display library

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

Cite this

Midoro-Horiuti, T., & Goldblum, R. M. (2014). Epitope mapping with random phage display library. In Methods in Molecular Biology (Vol. 1131, pp. 477-484). (Methods in Molecular Biology; Vol. 1131). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-992-5-28