Epitope mapping with random phage display library

Terumi Midoro-Horiuti, Randall M. Goldblum

Research output: Chapter in Book/Report/Conference proceedingChapter

5 Scopus citations

Abstract

Random phage display library is used to map conformational as well as linear epitopes. These libraries are available in varying lengths and with circularization. We provide here a protocol conveying our experience using a commercially available peptide phage display library, which in our hands provides good results.

Original languageEnglish (US)
Title of host publicationMonoclonal Antibodies
Subtitle of host publicationMethods and Protocols
PublisherHumana Press Inc.
Pages477-484
Number of pages8
ISBN (Print)9781627039918
DOIs
StatePublished - 2014

Publication series

NameMethods in Molecular Biology
Volume1131
ISSN (Print)1064-3745

Keywords

  • Allergen
  • Conformational epitope
  • Monoclonal antibody
  • Random phage display library

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics

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