Abstract
Random phage display library is used to map conformational as well as linear epitopes. These libraries are available in varying lengths and with circularization. We provide here a protocol conveying our experience using a commercially available peptide phage display library, which in our hands provides good results.
| Original language | English (US) |
|---|---|
| Title of host publication | Methods in Molecular Biology |
| Publisher | Humana Press Inc. |
| Pages | 477-484 |
| Number of pages | 8 |
| Volume | 1131 |
| ISBN (Print) | 9781627039918 |
| DOIs | |
| State | Published - 2014 |
Publication series
| Name | Methods in Molecular Biology |
|---|---|
| Volume | 1131 |
| ISSN (Print) | 10643745 |
Fingerprint
Keywords
- Allergen
- Conformational epitope
- Monoclonal antibody
- Random phage display library
ASJC Scopus subject areas
- Molecular Biology
- Genetics
Cite this
Epitope mapping with random phage display library. / Midoro-Horiuti, Terumi; Goldblum, Randall M.
Methods in Molecular Biology. Vol. 1131 Humana Press Inc., 2014. p. 477-484 (Methods in Molecular Biology; Vol. 1131).Research output: Chapter in Book/Report/Conference proceeding › Chapter
}
TY - CHAP
T1 - Epitope mapping with random phage display library
AU - Midoro-Horiuti, Terumi
AU - Goldblum, Randall M.
PY - 2014
Y1 - 2014
N2 - Random phage display library is used to map conformational as well as linear epitopes. These libraries are available in varying lengths and with circularization. We provide here a protocol conveying our experience using a commercially available peptide phage display library, which in our hands provides good results.
AB - Random phage display library is used to map conformational as well as linear epitopes. These libraries are available in varying lengths and with circularization. We provide here a protocol conveying our experience using a commercially available peptide phage display library, which in our hands provides good results.
KW - Allergen
KW - Conformational epitope
KW - Monoclonal antibody
KW - Random phage display library
UR - http://www.scopus.com/inward/record.url?scp=84934444589&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84934444589&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-992-5_28
DO - 10.1007/978-1-62703-992-5_28
M3 - Chapter
C2 - 24515483
AN - SCOPUS:84934444589
SN - 9781627039918
VL - 1131
T3 - Methods in Molecular Biology
SP - 477
EP - 484
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -