Epitope mapping with random phage display library

Terumi Midoro-Horiuti; Randall M. Goldblum

doi:10.1007/978-1-62703-992-5_28

Epitope mapping with random phage display library

Terumi Midoro-Horiuti, Randall M. Goldblum

Pediatrics

Research output: Chapter in Book/Report/Conference proceeding › Chapter

5 Scopus citations

Abstract

Random phage display library is used to map conformational as well as linear epitopes. These libraries are available in varying lengths and with circularization. We provide here a protocol conveying our experience using a commercially available peptide phage display library, which in our hands provides good results.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	477-484
Number of pages	8
Volume	1131
ISBN (Print)	9781627039918
DOIs	https://doi.org/10.1007/978-1-62703-992-5_28
State	Published - 2014

Publication series

Name	Methods in Molecular Biology
Volume	1131
ISSN (Print)	10643745

Keywords

Allergen
Conformational epitope
Monoclonal antibody
Random phage display library

ASJC Scopus subject areas

Molecular Biology
Genetics

Access to Document

10.1007/978-1-62703-992-5_28

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Midoro-Horiuti, T., & Goldblum, R. M. (2014). Epitope mapping with random phage display library. In Methods in Molecular Biology (Vol. 1131, pp. 477-484). (Methods in Molecular Biology; Vol. 1131). Humana Press Inc.. https://doi.org/10.1007/978-1-62703-992-5_28

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