Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China

Hiroki Kariwa, Cui Bai Zhong, Koichi Araki, Kumiko Yoshimatsu, Kumari Lokugamage, Nandadeva Lokugamage, Michael E. Murphy, Tetsuya Mizutani, Jiro Arikawa, Hiroshi Fukushima, Hu Xiong, Chen Jiehua, Ikuo Takashima

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Hantaviral antibodies were detected in the sera from Apodemus (A.) agrarius and A. peninsulae captured in Ningxia province, China by several different serological diagnostic methods. A total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (IFA). Among them, 19 of 191 (9.9%) sera of A. agrarius and 1 of 13 (7.7%) sera of A. peninsulae were positive for hantaviral antibodies. The other species (Rattus norvegicus, Mus musculus, Cricetulus triton, and Sorex cylindricauda) were negative. The reaction pattern of positive serum was characterized as scattered and granular virus antigens in the cytoplasm of hantavirus infected Vero E6 cells. Some of the A. agrarius sera positive for hantavirus were further examined by Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and the focus reduction neutralization test (FRNT). By WB, positive sera showed the same specific reaction pattern of baculovirus-expressed recombinant hantaviral nucleocapsid protein, as shown in hantavirus-immune serum. By ELISA, IFA-positive sera showed significantly higher optical densities (around 1.0) than the negative A. agrarius sera. Hantaan type hantavirus was neutralized with the positive sera. These results suggest that A. agrarius have hantavirus infection and may play a role as a reservoir animal for hantavirus in Ningxia Hui Autonomous Province, China.

Original languageEnglish (US)
Pages (from-to)105-114
Number of pages10
JournalJapanese Journal of Veterinary Research
Volume49
Issue number2
StatePublished - 2001
Externally publishedYes

Fingerprint

Hantavirus
blood serum
Rodentia
China
rodents
Serum
antibodies
Antibodies
Western blotting
Cricetulus
Hantavirus Infections
Enzyme-Linked Immunosorbent Assay
Surveys and Questionnaires
enzyme-linked immunosorbent assay
Far-Western Blotting
nucleocapsid proteins
Murinae
Nucleocapsid Proteins
Neutralization Tests
Apodemus

Keywords

  • Apodemus agrarius
  • China
  • Hantavirus
  • Hemorrhagic fever with renal syndrome
  • Rodent

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Kariwa, H., Zhong, C. B., Araki, K., Yoshimatsu, K., Lokugamage, K., Lokugamage, N., ... Takashima, I. (2001). Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China. Japanese Journal of Veterinary Research, 49(2), 105-114.

Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China. / Kariwa, Hiroki; Zhong, Cui Bai; Araki, Koichi; Yoshimatsu, Kumiko; Lokugamage, Kumari; Lokugamage, Nandadeva; Murphy, Michael E.; Mizutani, Tetsuya; Arikawa, Jiro; Fukushima, Hiroshi; Xiong, Hu; Jiehua, Chen; Takashima, Ikuo.

In: Japanese Journal of Veterinary Research, Vol. 49, No. 2, 2001, p. 105-114.

Research output: Contribution to journalArticle

Kariwa, H, Zhong, CB, Araki, K, Yoshimatsu, K, Lokugamage, K, Lokugamage, N, Murphy, ME, Mizutani, T, Arikawa, J, Fukushima, H, Xiong, H, Jiehua, C & Takashima, I 2001, 'Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China', Japanese Journal of Veterinary Research, vol. 49, no. 2, pp. 105-114.
Kariwa H, Zhong CB, Araki K, Yoshimatsu K, Lokugamage K, Lokugamage N et al. Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China. Japanese Journal of Veterinary Research. 2001;49(2):105-114.
Kariwa, Hiroki ; Zhong, Cui Bai ; Araki, Koichi ; Yoshimatsu, Kumiko ; Lokugamage, Kumari ; Lokugamage, Nandadeva ; Murphy, Michael E. ; Mizutani, Tetsuya ; Arikawa, Jiro ; Fukushima, Hiroshi ; Xiong, Hu ; Jiehua, Chen ; Takashima, Ikuo. / Epizootiological survey of hantavirus among rodent species in Ningxia Hui Autonomous Province, China. In: Japanese Journal of Veterinary Research. 2001 ; Vol. 49, No. 2. pp. 105-114.
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abstract = "Hantaviral antibodies were detected in the sera from Apodemus (A.) agrarius and A. peninsulae captured in Ningxia province, China by several different serological diagnostic methods. A total of 409 sera from rodent and insectivore species were collected in 1999 and examined by indirect immunofluorescent antibody assay (IFA). Among them, 19 of 191 (9.9{\%}) sera of A. agrarius and 1 of 13 (7.7{\%}) sera of A. peninsulae were positive for hantaviral antibodies. The other species (Rattus norvegicus, Mus musculus, Cricetulus triton, and Sorex cylindricauda) were negative. The reaction pattern of positive serum was characterized as scattered and granular virus antigens in the cytoplasm of hantavirus infected Vero E6 cells. Some of the A. agrarius sera positive for hantavirus were further examined by Western blotting (WB), enzyme-linked immunosorbent assay (ELISA), and the focus reduction neutralization test (FRNT). By WB, positive sera showed the same specific reaction pattern of baculovirus-expressed recombinant hantaviral nucleocapsid protein, as shown in hantavirus-immune serum. By ELISA, IFA-positive sera showed significantly higher optical densities (around 1.0) than the negative A. agrarius sera. Hantaan type hantavirus was neutralized with the positive sera. These results suggest that A. agrarius have hantavirus infection and may play a role as a reservoir animal for hantavirus in Ningxia Hui Autonomous Province, China.",
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AU - Lokugamage, Nandadeva

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