Epoxyeicosatrienoic acid metabolism in arterial smooth muscle cells

Xiang Fang, M. VanRollins, T. L. Kaduce, A. A. Spector

Research output: Contribution to journalArticle

49 Scopus citations

Abstract

Epoxyeicosatrienoic acids (EETs) are eicosanoids synthesized from arachidonic acid by the cytochrome P450 epoxygenase pathway. The present studies demonstrate that 8,9-, 11.12-, and 14,15-EET are rapidly taken up by porcine aortic smooth muscle cells. About half of the uptake is incorporated into phospholipids, and saponification indicates that most of this remains in the form of EET. The EETs also are converted to the corresponding dihydroxyeicosatrienoic acids (DHETs) and during prolonged incubations, additional metabolites that do not retain the EET carboxyl groups are formed. Most of these products are released into the medium. However, some DHET and metabolites less polar than EET are incorporated into the phospholipids, and a small amount of unesterified EET is also present in the cells. The incorporation of 14,15-EET and its conversion to DHET did not approach saturation until the concentration exceeded 10-20 μM, indicating that vascular smooth muscle has a large capacity to utilize this EET. These findings suggest that certain vasoactive effects of EETs may be due to their incorporation by smooth muscle cells. Furthermore, through conversion to DHET and other oxidized metabolites, smooth muscle apparently has the capacity to inactive EETs that are either formed in or penetrate into the vascular wall.

Original languageEnglish (US)
Pages (from-to)1236-1246
Number of pages11
JournalJournal of Lipid Research
Volume36
Issue number6
StatePublished - 1995
Externally publishedYes

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Keywords

  • arachidonic acid
  • dihydroxyeicosatrienoic acid
  • eicosanoids
  • epoxides
  • phospholipids

ASJC Scopus subject areas

  • Endocrinology

Cite this

Fang, X., VanRollins, M., Kaduce, T. L., & Spector, A. A. (1995). Epoxyeicosatrienoic acid metabolism in arterial smooth muscle cells. Journal of Lipid Research, 36(6), 1236-1246.