Epoxyficosatrienoic acids (EETs) inhibit the formation of prostaglandin E2 by smooth muscle cells

Xiang Fang, T. L. Kaduce, N. L. Weintraub, L. L. Stoll, A. A. Spector

Research output: Contribution to journalArticle

Abstract

Epoxyeicosatrienoic acids (EETs) and prostaglandins (PG) are eicosanoids derived from arachidonic acid (AA) by cytochrome P450 epoxygenase and cycloxygenase metabolism, respectively. EETs were previously reported to inhibit PG production by platelets and blood vessels. To determine whether EETs can inhibit PG production by vascular smooth muscle cells (SMC), porcine aortic SMC were incubated with [3H]AA in the presence and absence of EETs, and PG production in the medium was determined by reverse-phase HPLC and radioimmunoassay (RIA). The formation of PGE2, the major PG produced by SMC, was inhibited in concentration-dependent fashion by 14,15-EET. In the presence of 1 μM 14,15-EET, basal PGE2 formation, and PGE2 formation stimulated by 2 μM calcium ionophore A23187, were reduced by 40-50%. 8,9-EET (1 μM) decreased PGE2 formation by 30%, whereas 11,12-EET and 5,6-EET were without effect. 14,15-dihydroxyeicosatrienoic acid (DHET), the major metabolite of 14,15-EET, and 15-(S)-hydroxyeicosatetraenoic acid also did not alter PGE2 production by SMC. 14,15-EET-induced inhibition of PGE2 production was diminished by the inclusion of 30 μM AA. 4-Phenylchalcone oxide, a potent inhibitor of epoxide hydrolase (the enzyme responsible for conversion of EETs to DHETs), potentiated the 14,15-EET-induced inhibition of PGE2 production. We conclude that EETs regio-specifically inhibit PGE2 production by SMC.

Original languageEnglish (US)
JournalFASEB Journal
Volume11
Issue number3
StatePublished - 1997
Externally publishedYes

Fingerprint

Dinoprostone
smooth muscle
myocytes
prostaglandins
Smooth Muscle Myocytes
Muscle
Cells
Prostaglandins
Acids
arachidonic acid
acids
Arachidonic Acid
blood vessels
epoxide hydrolase
eicosanoids
ionophores
radioimmunoassays
cytochrome P-450
Epoxide Hydrolases
Hydroxyeicosatetraenoic Acids

ASJC Scopus subject areas

  • Agricultural and Biological Sciences (miscellaneous)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Biochemistry
  • Cell Biology

Cite this

Fang, X., Kaduce, T. L., Weintraub, N. L., Stoll, L. L., & Spector, A. A. (1997). Epoxyficosatrienoic acids (EETs) inhibit the formation of prostaglandin E2 by smooth muscle cells. FASEB Journal, 11(3).

Epoxyficosatrienoic acids (EETs) inhibit the formation of prostaglandin E2 by smooth muscle cells. / Fang, Xiang; Kaduce, T. L.; Weintraub, N. L.; Stoll, L. L.; Spector, A. A.

In: FASEB Journal, Vol. 11, No. 3, 1997.

Research output: Contribution to journalArticle

Fang, X, Kaduce, TL, Weintraub, NL, Stoll, LL & Spector, AA 1997, 'Epoxyficosatrienoic acids (EETs) inhibit the formation of prostaglandin E2 by smooth muscle cells', FASEB Journal, vol. 11, no. 3.
Fang, Xiang ; Kaduce, T. L. ; Weintraub, N. L. ; Stoll, L. L. ; Spector, A. A. / Epoxyficosatrienoic acids (EETs) inhibit the formation of prostaglandin E2 by smooth muscle cells. In: FASEB Journal. 1997 ; Vol. 11, No. 3.
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AU - Fang, Xiang

AU - Kaduce, T. L.

AU - Weintraub, N. L.

AU - Stoll, L. L.

AU - Spector, A. A.

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