Eps15 and Dap160 control synaptic vesicle membrane retrieval and synapse development

Tong Wey Koh, Viktor I. Korolchuk, Yogesh P. Wairkar, Wei Jiao, Emma Evergren, Hongling Pan, Yi Zhou, Koen J.T. Venken, Oleg Shupliakov, Iain M. Robinson, Cahir J. O'Kane, Hugo J. Bellen

Research output: Contribution to journalArticlepeer-review

107 Scopus citations


Epidermal growth factor receptor pathway substrate clone 15 (Eps15) is a protein implicated in endocytosis, endosomal protein sorting, and cytoskeletal organization. Its role is, however, still unclear, because of reasons including limitations of dominant-negative experiments and apparent redundancy with other endocytic proteins. We generated Drosophila eps15-null mutants and show that Eps15 is required for proper synaptic bouton development and normal levels of synaptic vesicle (SV) endocytosis. Consistent with a role in SV endocytosis, Eps15 moves from the center of synaptic boutons to the periphery in response to synaptic activity. The endocytic protein, Dap160/intersectin, is a major binding partner of Eps15, and eps15 mutants phenotypically resemble dap160 mutants. Analyses of eps15 dap160 double mutants suggest that Eps15 functions in concert with Dap160 during SV endocytosis. Based on these data, we hypothesize that Eps15 and Dap160 promote the efficiency of endocytosis from the plasma membrane by maintaining high concentrations of multiple endocytic proteins, including dynamin, at synapses.

Original languageEnglish (US)
Pages (from-to)309-322
Number of pages14
JournalJournal of Cell Biology
Issue number2
StatePublished - Jul 16 2007
Externally publishedYes

ASJC Scopus subject areas

  • Cell Biology


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