Error-free replicative bypass of (6-4) photoproducts by DNA polymerase ζ in mouse and human cells

Jung Hoon Yoon, Louise Prakash, Satya Prakash

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

The ultraviolet (UV)-induced (6-4) pyrimidine-pyrimidone photoproduct [(6-4) PP] confers a large structural distortion in DNA. Here we examine in human cells the roles of translesion synthesis (TLS) DNA polymerases (Pols) in promoting replication through a (6-4) TT photoproduct carried on a duplex plasmid where bidirectional replication initiates from an origin of replication. We show that TLS contributes to a large fraction of lesion bypass and that it ismostly error-free. We find that, whereas Pol h and Pol i provide alternate pathways for mutagenic TLS, surprisingly, Pol ζ functions independently of these Pols and in a predominantly error-free manner. We verify and extend these observations in mouse cells and conclude that, in human cells, TLS during replication can be markedly error-free even opposite a highly distorting DNA lesion.

Original languageEnglish (US)
Pages (from-to)123-128
Number of pages6
JournalGenes and Development
Volume24
Issue number2
DOIs
StatePublished - Jan 15 2010

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DNA-Directed DNA Polymerase
Replication Origin
DNA
Plasmids

Keywords

  • (6-4) photoproducts
  • DNA repair
  • DNA replication
  • Replicative lesion bypass
  • UV damage

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

Cite this

Error-free replicative bypass of (6-4) photoproducts by DNA polymerase ζ in mouse and human cells. / Yoon, Jung Hoon; Prakash, Louise; Prakash, Satya.

In: Genes and Development, Vol. 24, No. 2, 15.01.2010, p. 123-128.

Research output: Contribution to journalArticle

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