Erythrocyte-aniline interaction leads to their accumulation and iron deposition in rat spleen

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Abstract

In order to understand the splenic toxicity of anline in rats, early interaction of anline with erythrocytes and its subsequent deposition and covalent binding to macromolecules in target (spleen) and nontarget (liver) organs have been studied. Mate Sprague-Dawley (SD) rats were given 1 or 3 doses of 1 mmol/kg [14C]aniline hydrochloride (1 doseld) by gavage and euthanized 24 h after the treatment. Among blood components, maximum radioactivity was found to be associated with red blood cells (RBCs). After 3 doses, there was 112, 79, and 67% increase In the radioactivity in the whole blood, RBCs, and hemolysate, respectively, in comparison to 1 dose. In comparison to RBCs, plasma had only 40 and 76% radioactivity after the administration of 1 and 3 doses, respectively. Spleen homogenate at 1 dose had one-third of tile radioactivity in tile TCA precipitate, which increased to 40% at 3 doses, while the total radioactivity increased 256% over 1 dose. Liver, which had almost double tile radioactivity on a per gram tissue basis compared to the spleen at one dose, did not show any appreciable increase in the radioactivity at three doses. However, radioactivity in the TCA precipitate of liver homogenate increased by 92% after 3 doses. The iron content of the spleen in rats given 3 doses of [14C]anline increased by 85% compared to the rats given just 1 dose. The iron content of liver did not show any change at three doses. These data thus demonstrate a dose-dependent binding and accumulation of radioactivity in erythrocytes and spleen. These interactions, along with parallel increases in the iron content of the spleen, could be critical in the splenic toxicity of anilines.

Original languageEnglish (US)
Pages (from-to)415-421
Number of pages7
JournalJournal of Toxicology and Environmental Health
Volume44
Issue number4
StatePublished - 1995

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Radioactivity
Aniline
Rats
Spleen
Iron
Erythrocytes
Dosimetry
Blood
Liver
Tile
Toxicity
Precipitates
Aniline Compounds
aniline
Macromolecules
Sprague Dawley Rats
Cells
Tissue
Plasmas

ASJC Scopus subject areas

  • Toxicology
  • Pollution

Cite this

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title = "Erythrocyte-aniline interaction leads to their accumulation and iron deposition in rat spleen",
abstract = "In order to understand the splenic toxicity of anline in rats, early interaction of anline with erythrocytes and its subsequent deposition and covalent binding to macromolecules in target (spleen) and nontarget (liver) organs have been studied. Mate Sprague-Dawley (SD) rats were given 1 or 3 doses of 1 mmol/kg [14C]aniline hydrochloride (1 doseld) by gavage and euthanized 24 h after the treatment. Among blood components, maximum radioactivity was found to be associated with red blood cells (RBCs). After 3 doses, there was 112, 79, and 67{\%} increase In the radioactivity in the whole blood, RBCs, and hemolysate, respectively, in comparison to 1 dose. In comparison to RBCs, plasma had only 40 and 76{\%} radioactivity after the administration of 1 and 3 doses, respectively. Spleen homogenate at 1 dose had one-third of tile radioactivity in tile TCA precipitate, which increased to 40{\%} at 3 doses, while the total radioactivity increased 256{\%} over 1 dose. Liver, which had almost double tile radioactivity on a per gram tissue basis compared to the spleen at one dose, did not show any appreciable increase in the radioactivity at three doses. However, radioactivity in the TCA precipitate of liver homogenate increased by 92{\%} after 3 doses. The iron content of the spleen in rats given 3 doses of [14C]anline increased by 85{\%} compared to the rats given just 1 dose. The iron content of liver did not show any change at three doses. These data thus demonstrate a dose-dependent binding and accumulation of radioactivity in erythrocytes and spleen. These interactions, along with parallel increases in the iron content of the spleen, could be critical in the splenic toxicity of anilines.",
author = "M Khan and Bhupendra Kaphalia and Ghulam Ansari",
year = "1995",
language = "English (US)",
volume = "44",
pages = "415--421",
journal = "Journal of Toxicology and Environmental Health - Part A: Current Issues",
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TY - JOUR

T1 - Erythrocyte-aniline interaction leads to their accumulation and iron deposition in rat spleen

AU - Khan, M

AU - Kaphalia, Bhupendra

AU - Ansari, Ghulam

PY - 1995

Y1 - 1995

N2 - In order to understand the splenic toxicity of anline in rats, early interaction of anline with erythrocytes and its subsequent deposition and covalent binding to macromolecules in target (spleen) and nontarget (liver) organs have been studied. Mate Sprague-Dawley (SD) rats were given 1 or 3 doses of 1 mmol/kg [14C]aniline hydrochloride (1 doseld) by gavage and euthanized 24 h after the treatment. Among blood components, maximum radioactivity was found to be associated with red blood cells (RBCs). After 3 doses, there was 112, 79, and 67% increase In the radioactivity in the whole blood, RBCs, and hemolysate, respectively, in comparison to 1 dose. In comparison to RBCs, plasma had only 40 and 76% radioactivity after the administration of 1 and 3 doses, respectively. Spleen homogenate at 1 dose had one-third of tile radioactivity in tile TCA precipitate, which increased to 40% at 3 doses, while the total radioactivity increased 256% over 1 dose. Liver, which had almost double tile radioactivity on a per gram tissue basis compared to the spleen at one dose, did not show any appreciable increase in the radioactivity at three doses. However, radioactivity in the TCA precipitate of liver homogenate increased by 92% after 3 doses. The iron content of the spleen in rats given 3 doses of [14C]anline increased by 85% compared to the rats given just 1 dose. The iron content of liver did not show any change at three doses. These data thus demonstrate a dose-dependent binding and accumulation of radioactivity in erythrocytes and spleen. These interactions, along with parallel increases in the iron content of the spleen, could be critical in the splenic toxicity of anilines.

AB - In order to understand the splenic toxicity of anline in rats, early interaction of anline with erythrocytes and its subsequent deposition and covalent binding to macromolecules in target (spleen) and nontarget (liver) organs have been studied. Mate Sprague-Dawley (SD) rats were given 1 or 3 doses of 1 mmol/kg [14C]aniline hydrochloride (1 doseld) by gavage and euthanized 24 h after the treatment. Among blood components, maximum radioactivity was found to be associated with red blood cells (RBCs). After 3 doses, there was 112, 79, and 67% increase In the radioactivity in the whole blood, RBCs, and hemolysate, respectively, in comparison to 1 dose. In comparison to RBCs, plasma had only 40 and 76% radioactivity after the administration of 1 and 3 doses, respectively. Spleen homogenate at 1 dose had one-third of tile radioactivity in tile TCA precipitate, which increased to 40% at 3 doses, while the total radioactivity increased 256% over 1 dose. Liver, which had almost double tile radioactivity on a per gram tissue basis compared to the spleen at one dose, did not show any appreciable increase in the radioactivity at three doses. However, radioactivity in the TCA precipitate of liver homogenate increased by 92% after 3 doses. The iron content of the spleen in rats given 3 doses of [14C]anline increased by 85% compared to the rats given just 1 dose. The iron content of liver did not show any change at three doses. These data thus demonstrate a dose-dependent binding and accumulation of radioactivity in erythrocytes and spleen. These interactions, along with parallel increases in the iron content of the spleen, could be critical in the splenic toxicity of anilines.

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