Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults

Séverine Lamon, Evelyn Zacharewicz, Emily Arentson-Lantz, Paul A Della Gatta, Lobna Ghobrial, Frederico Gerlinger-Romero, Andrew Garnham, Douglas Paddon-Jones, Aaron P. Russell

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Purpose: Erythropoietin (EPO) is a renal cytokine that is primarily involved in hematopoiesis while also playing a role in non-hematopoietic tissues expressing the EPO-receptor (EPOR). The EPOR is present in human skeletal muscle. In mouse skeletal muscle, EPO stimulation can activate the AKT serine/threonine kinase 1 (AKT) signaling pathway, the main positive regulator of muscle protein synthesis. We hypothesized that a single intravenous EPO injection combined with acute resistance exercise would have a synergistic effect on skeletal muscle protein synthesis via activation of the AKT pathway. Methods: Ten young (24.2 ± 0.9 years) and 10 older (66.6 ± 1.1 years) healthy subjects received a primed, constant infusion of [ring-13C6] L-phenylalanine and a single injection of 10,000 IU epoetin-beta or placebo in a double-blind randomized, cross-over design. 2 h after the injection, the subjects completed an acute bout of leg extension resistance exercise to stimulate skeletal muscle protein synthesis. Results: Significant interaction effects in the phosphorylation levels of the members of the AKT signaling pathway indicated a differential activation of protein synthesis signaling in older subjects when compared to young subjects. However, EPO offered no synergistic effect on vastus lateralis mixed muscle protein synthesis rate in young or older subjects. Conclusions: Despite its ability to activate the AKT pathway in skeletal muscle, an acute EPO injection had no additive or synergistic effect on the exercise-induced activation of muscle protein synthesis or muscle protein synthesis signaling pathways.

Original languageEnglish (US)
Article number292
JournalFrontiers in Physiology
Volume7
Issue numberJUL
DOIs
StatePublished - Jul 8 2016

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Muscle Proteins
Erythropoietin
Young Adult
Skeletal Muscle
Exercise
Injections
Erythropoietin Receptors
Protein-Serine-Threonine Kinases
Quadriceps Muscle
Hematopoiesis
Phenylalanine
Intravenous Injections
Cross-Over Studies
Leg
Healthy Volunteers
Placebos
Phosphorylation
Cytokines
Kidney
Proteins

Keywords

  • Aging
  • Anabolic signaling
  • Erythropoietin
  • Muscle protein synthesis
  • Resistance exercise

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

Cite this

Lamon, S., Zacharewicz, E., Arentson-Lantz, E., Gatta, P. A. D., Ghobrial, L., Gerlinger-Romero, F., ... Russell, A. P. (2016). Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults. Frontiers in Physiology, 7(JUL), [292]. https://doi.org/10.3389/fphys.2016.00292

Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults. / Lamon, Séverine; Zacharewicz, Evelyn; Arentson-Lantz, Emily; Gatta, Paul A Della; Ghobrial, Lobna; Gerlinger-Romero, Frederico; Garnham, Andrew; Paddon-Jones, Douglas; Russell, Aaron P.

In: Frontiers in Physiology, Vol. 7, No. JUL, 292, 08.07.2016.

Research output: Contribution to journalArticle

Lamon, S, Zacharewicz, E, Arentson-Lantz, E, Gatta, PAD, Ghobrial, L, Gerlinger-Romero, F, Garnham, A, Paddon-Jones, D & Russell, AP 2016, 'Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults', Frontiers in Physiology, vol. 7, no. JUL, 292. https://doi.org/10.3389/fphys.2016.00292
Lamon S, Zacharewicz E, Arentson-Lantz E, Gatta PAD, Ghobrial L, Gerlinger-Romero F et al. Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults. Frontiers in Physiology. 2016 Jul 8;7(JUL). 292. https://doi.org/10.3389/fphys.2016.00292
Lamon, Séverine ; Zacharewicz, Evelyn ; Arentson-Lantz, Emily ; Gatta, Paul A Della ; Ghobrial, Lobna ; Gerlinger-Romero, Frederico ; Garnham, Andrew ; Paddon-Jones, Douglas ; Russell, Aaron P. / Erythropoietin does not enhance skeletal muscle protein synthesis following exercise in young and older adults. In: Frontiers in Physiology. 2016 ; Vol. 7, No. JUL.
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AU - Ghobrial, Lobna

AU - Gerlinger-Romero, Frederico

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