Escherichia coli bacteriophage Gostya9, representing a new species within the genus T5virus

Alla K. Golomidova, Eugene E. Kulikov, Vladislav V. Babenko, Pavel A. Ivanov, Nikolai Prokhorov, Andrey V. Letarov

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Escherichia coli bacteriophage Gostya9 (genus T5virus) was isolated from horse feces collected in Moscow, Russia, in 2013. This phage was associated in a single plaque with the previously reported phage 9g and was subsequently purified. Analysis of the complete genomic sequence of Gostya9 revealed that it is closely related to the T5-like bacteriophage DT57C, which had been isolated at the same location in 2007. These two viruses share 79.5% nucleotide sequence identity, which is below the 95% threshold applied currently to demarcate bacteriophage species. The most significant features distinguishing Gostya9 from DT57C include 1) the presence of one long tail fiber protein gene, 122c (ltf), instead of the two genes, ltfA and ltfB, that are present in DT57C; 2) the absence of the gene for the receptor-blocking lytic conversion lipoprotein precursor llp; and 3) the divergence of the receptor-recognition protein, pb5, which is only distantly related at the amino acid sequence level. The observed features of the Gostya9 adsorption apparatus are suggestive of a possible novel specificity for the final receptor and make this phage interesting for possible direct application in phage therapy of E. coli infections or as a source of receptor-recognition protein for engineering new phage specificities.

Original languageEnglish (US)
JournalArchives of Virology
DOIs
StateAccepted/In press - Jan 1 2018

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Bacteriophages
Escherichia coli
Virus Receptors
Protein Engineering
Escherichia coli Infections
Moscow
Russia
Feces
Genes
Lipoproteins
Adsorption
Horses
Tail
Amino Acid Sequence
Proteins
Viruses

ASJC Scopus subject areas

  • Virology

Cite this

Golomidova, A. K., Kulikov, E. E., Babenko, V. V., Ivanov, P. A., Prokhorov, N., & Letarov, A. V. (Accepted/In press). Escherichia coli bacteriophage Gostya9, representing a new species within the genus T5virus. Archives of Virology. https://doi.org/10.1007/s00705-018-4113-2

Escherichia coli bacteriophage Gostya9, representing a new species within the genus T5virus. / Golomidova, Alla K.; Kulikov, Eugene E.; Babenko, Vladislav V.; Ivanov, Pavel A.; Prokhorov, Nikolai; Letarov, Andrey V.

In: Archives of Virology, 01.01.2018.

Research output: Contribution to journalArticle

Golomidova, Alla K. ; Kulikov, Eugene E. ; Babenko, Vladislav V. ; Ivanov, Pavel A. ; Prokhorov, Nikolai ; Letarov, Andrey V. / Escherichia coli bacteriophage Gostya9, representing a new species within the genus T5virus. In: Archives of Virology. 2018.
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