Essential amino acids increase microRNA-499, -208b, and -23a and downregulate myostatin and myocyte enhancer factor 2C mRNA expression in human skeletal muscle

Micah J. Drummond, Erin L. Glynn, Christopher Fry, Shaheen Dhanani, Elena Volpi, Blake Rasmussen

Research output: Contribution to journalArticle

79 Citations (Scopus)

Abstract

Essential amino acids (EAA) stimulate muscle protein synthesis in humans. However, little is known about whether microRNAs (miRNA) and genes associated with muscle growth are expressed differently following EAA ingestion. Our purpose in this experiment was to determine whether miRNA and growth-related mRNA expressed in skeletal muscle are up- or downregulated in humans following the ingestion of EAA. We hypothesized that EAA would alter miRNA expression in skeletal muscle as well as select growth-related genes. Muscle biopsies were obtained from the vastus lateralis of 7 young adult participants (3 male, 4 female) before and 3 h after ingesting 10 g of EAA. Muscle samples were analyzed for muscle miRNA (miR-499, -208b, -23a, -1, -133a, and -206) and muscle-growth related genes [MyoD1, myogenin, myostatin, myocyte enhancer factor C (MEF2C), follistatin-like-1 (FSTL1), histone deacytylase 4, and serum response factor mRNA] before and after EAA ingestion using real-time PCR. Following EAA ingestion, miR-499, -208b, -23a, -1, and pri-miR-206 expression increased (P , 0.05). The muscle-growth genes MyoD1 and FSTL1 mRNA expression increased (P , 0.05), and myostatin and MEF2C mRNA were downregulated following EAA ingestion (P , 0.05). We conclude that miRNA and growth-related genes expressed in skeletal muscle are rapidly altered within hours following EAA ingestion. Further work is needed to determine whether these miRNA are post-transcriptional regulators of growth-related genes following an anabolic stimulus.

Original languageEnglish (US)
Pages (from-to)2279-2284
Number of pages6
JournalJournal of Nutrition
Volume139
Issue number12
DOIs
StatePublished - Dec 2009

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MEF2 Transcription Factors
Myostatin
Essential Amino Acids
MicroRNAs
Skeletal Muscle
Down-Regulation
Messenger RNA
Eating
Muscles
Growth
Follistatin
Genes
Serum Response Factor
Myogenin
Muscle Proteins
Quadriceps Muscle
Histones
Muscle Cells
Real-Time Polymerase Chain Reaction
Young Adult

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Nutrition and Dietetics

Cite this

Essential amino acids increase microRNA-499, -208b, and -23a and downregulate myostatin and myocyte enhancer factor 2C mRNA expression in human skeletal muscle. / Drummond, Micah J.; Glynn, Erin L.; Fry, Christopher; Dhanani, Shaheen; Volpi, Elena; Rasmussen, Blake.

In: Journal of Nutrition, Vol. 139, No. 12, 12.2009, p. 2279-2284.

Research output: Contribution to journalArticle

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abstract = "Essential amino acids (EAA) stimulate muscle protein synthesis in humans. However, little is known about whether microRNAs (miRNA) and genes associated with muscle growth are expressed differently following EAA ingestion. Our purpose in this experiment was to determine whether miRNA and growth-related mRNA expressed in skeletal muscle are up- or downregulated in humans following the ingestion of EAA. We hypothesized that EAA would alter miRNA expression in skeletal muscle as well as select growth-related genes. Muscle biopsies were obtained from the vastus lateralis of 7 young adult participants (3 male, 4 female) before and 3 h after ingesting 10 g of EAA. Muscle samples were analyzed for muscle miRNA (miR-499, -208b, -23a, -1, -133a, and -206) and muscle-growth related genes [MyoD1, myogenin, myostatin, myocyte enhancer factor C (MEF2C), follistatin-like-1 (FSTL1), histone deacytylase 4, and serum response factor mRNA] before and after EAA ingestion using real-time PCR. Following EAA ingestion, miR-499, -208b, -23a, -1, and pri-miR-206 expression increased (P , 0.05). The muscle-growth genes MyoD1 and FSTL1 mRNA expression increased (P , 0.05), and myostatin and MEF2C mRNA were downregulated following EAA ingestion (P , 0.05). We conclude that miRNA and growth-related genes expressed in skeletal muscle are rapidly altered within hours following EAA ingestion. Further work is needed to determine whether these miRNA are post-transcriptional regulators of growth-related genes following an anabolic stimulus.",
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