Essential role of microfibrillar-associated protein 4 in human cutaneous homeostasis and in its photoprotection

Shinya Kasamatsu, Akira Hachiya, Tsutomu Fujimura, Penkanok Sriwiriyanont, Keiichi Haketa, Marty O. Visscher, William J. Kitzmiller, Alexander Bello, Takashi Kitahara, Gary P. Kobinger, Yoshinori Takema

Research output: Contribution to journalArticlepeer-review

48 Scopus citations

Abstract

UVB-induced cutaneous photodamage/photoaging is characterized by qualitative and quantitative deterioration in dermal extracellular matrix (ECM) components such as collagen and elastic fibers. Disappearance of microfibrillar-associated protein 4 (MFAP-4), a possible limiting factor for cutaneous elasticity, was documented in photoaged dermis, but its function is poorly understood. To characterize its possible contribution to photoprotection, MFAP-4 expression was either augmented or inhibited in a human skin xenograft photodamage murine model and human fibroblasts. Xenografted skin with enhanced MFAP-4 expression was protected from UVB-induced photodamage/photoaging accompanied by the prevention of ECM degradation and aggravated elasticity. Additionally, remarkably increased or decreased fibrillin-1-based microfibril development was observed when fibroblasts were treated with recombinant MFAP-4 or with MFAP-4-specific siRNA, respectively. Immunoprecipitation analysis confirmed direct interaction between MFAP-4 and fibrillin-1. Taken together, our findings reveal the essential role of MFAP-4 in photoprotection and offer new therapeutic opportunities to prevent skin-associated pathologies.

Original languageEnglish (US)
Article number164
JournalScientific reports
Volume1
DOIs
StatePublished - 2011
Externally publishedYes

ASJC Scopus subject areas

  • General

Fingerprint

Dive into the research topics of 'Essential role of microfibrillar-associated protein 4 in human cutaneous homeostasis and in its photoprotection'. Together they form a unique fingerprint.

Cite this