Essentials of filoviral load quantification

Lieselotte Cnops; Johan van Griensven; Anna N. Honko; Daniel G. Bausch; Armand Sprecher; Charles E. Hill; Robert Colebunders; Joshua C. Johnson; Anthony Griffiths; Gustavo F. Palacios; Colleen S. Kraft; Gary Kobinger; Angela Hewlett; David A. Norwood; Pardis Sabeti; Peter B. Jahrling; Pierre Formenty; Jens H. Kuhn; Kevin K. Ariën

doi:10.1016/S1473-3099(16)30063-9

Essentials of filoviral load quantification

Lieselotte Cnops, Johan van Griensven, Anna N. Honko, Daniel G. Bausch, Armand Sprecher, Charles E. Hill, Robert Colebunders, Joshua C. Johnson, Anthony Griffiths, Gustavo F. Palacios, Colleen S. Kraft, Gary Kobinger, Angela Hewlett, David A. Norwood, Pardis Sabeti, Peter B. Jahrling, Pierre Formenty, Jens H. Kuhn, Kevin K. Ariën

Research output: Contribution to journal › Review article › peer-review

11 Scopus citations

Abstract

Quantitative measurement of viral load is an important parameter in the management of filovirus disease outbreaks because viral load correlates with severity of disease, survival, and infectivity. During the ongoing Ebola virus disease outbreak in parts of Western Africa, most assays used in the detection of Ebola virus disease by more than 44 diagnostic laboratories yielded qualitative results. Regulatory hurdles involved in validating quantitative assays and the urgent need for a rapid Ebola virus disease diagnosis precluded development of validated quantitative assays during the outbreak. Because of sparse quantitative data obtained from these outbreaks, opportunities for study of correlations between patient outcome, changes in viral load during the course of an outbreak, disease course in asymptomatic individuals, and the potential for virus transmission between infected patients and contacts have been limited. We strongly urge the continued development of quantitative viral load assays to carefully evaluate these parameters in future outbreaks of filovirus disease.

Original language	English (US)
Pages (from-to)	e134-e138
Journal	The Lancet Infectious Diseases
Volume	16
Issue number	7
DOIs	https://doi.org/10.1016/S1473-3099(16)30063-9
State	Published - Jul 1 2016
Externally published	Yes

ASJC Scopus subject areas

Infectious Diseases

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10.1016/S1473-3099(16)30063-9

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Cnops, L., van Griensven, J., Honko, A. N., Bausch, D. G., Sprecher, A., Hill, C. E., Colebunders, R., Johnson, J. C., Griffiths, A., Palacios, G. F., Kraft, C. S., Kobinger, G., Hewlett, A., Norwood, D. A., Sabeti, P., Jahrling, P. B., Formenty, P., Kuhn, J. H., & Ariën, K. K. (2016). Essentials of filoviral load quantification. The Lancet Infectious Diseases, 16(7), e134-e138. https://doi.org/10.1016/S1473-3099(16)30063-9

Cnops, L, van Griensven, J, Honko, AN, Bausch, DG, Sprecher, A, Hill, CE, Colebunders, R, Johnson, JC, Griffiths, A, Palacios, GF, Kraft, CS, Kobinger, G, Hewlett, A, Norwood, DA, Sabeti, P, Jahrling, PB, Formenty, P, Kuhn, JH & Ariën, KK 2016, 'Essentials of filoviral load quantification', The Lancet Infectious Diseases, vol. 16, no. 7, pp. e134-e138. https://doi.org/10.1016/S1473-3099(16)30063-9

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abstract = "Quantitative measurement of viral load is an important parameter in the management of filovirus disease outbreaks because viral load correlates with severity of disease, survival, and infectivity. During the ongoing Ebola virus disease outbreak in parts of Western Africa, most assays used in the detection of Ebola virus disease by more than 44 diagnostic laboratories yielded qualitative results. Regulatory hurdles involved in validating quantitative assays and the urgent need for a rapid Ebola virus disease diagnosis precluded development of validated quantitative assays during the outbreak. Because of sparse quantitative data obtained from these outbreaks, opportunities for study of correlations between patient outcome, changes in viral load during the course of an outbreak, disease course in asymptomatic individuals, and the potential for virus transmission between infected patients and contacts have been limited. We strongly urge the continued development of quantitative viral load assays to carefully evaluate these parameters in future outbreaks of filovirus disease.",

author = "Lieselotte Cnops and {van Griensven}, Johan and Honko, {Anna N.} and Bausch, {Daniel G.} and Armand Sprecher and Hill, {Charles E.} and Robert Colebunders and Johnson, {Joshua C.} and Anthony Griffiths and Palacios, {Gustavo F.} and Kraft, {Colleen S.} and Gary Kobinger and Angela Hewlett and Norwood, {David A.} and Pardis Sabeti and Jahrling, {Peter B.} and Pierre Formenty and Kuhn, {Jens H.} and Ari{\"e}n, {Kevin K.}",

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AU - Honko, Anna N.

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AU - Sprecher, Armand

AU - Hill, Charles E.

AU - Colebunders, Robert

AU - Johnson, Joshua C.

AU - Griffiths, Anthony

AU - Palacios, Gustavo F.

AU - Kraft, Colleen S.

AU - Kobinger, Gary

AU - Hewlett, Angela

AU - Norwood, David A.

AU - Sabeti, Pardis

AU - Jahrling, Peter B.

AU - Formenty, Pierre

AU - Kuhn, Jens H.

AU - Ariën, Kevin K.

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N2 - Quantitative measurement of viral load is an important parameter in the management of filovirus disease outbreaks because viral load correlates with severity of disease, survival, and infectivity. During the ongoing Ebola virus disease outbreak in parts of Western Africa, most assays used in the detection of Ebola virus disease by more than 44 diagnostic laboratories yielded qualitative results. Regulatory hurdles involved in validating quantitative assays and the urgent need for a rapid Ebola virus disease diagnosis precluded development of validated quantitative assays during the outbreak. Because of sparse quantitative data obtained from these outbreaks, opportunities for study of correlations between patient outcome, changes in viral load during the course of an outbreak, disease course in asymptomatic individuals, and the potential for virus transmission between infected patients and contacts have been limited. We strongly urge the continued development of quantitative viral load assays to carefully evaluate these parameters in future outbreaks of filovirus disease.

AB - Quantitative measurement of viral load is an important parameter in the management of filovirus disease outbreaks because viral load correlates with severity of disease, survival, and infectivity. During the ongoing Ebola virus disease outbreak in parts of Western Africa, most assays used in the detection of Ebola virus disease by more than 44 diagnostic laboratories yielded qualitative results. Regulatory hurdles involved in validating quantitative assays and the urgent need for a rapid Ebola virus disease diagnosis precluded development of validated quantitative assays during the outbreak. Because of sparse quantitative data obtained from these outbreaks, opportunities for study of correlations between patient outcome, changes in viral load during the course of an outbreak, disease course in asymptomatic individuals, and the potential for virus transmission between infected patients and contacts have been limited. We strongly urge the continued development of quantitative viral load assays to carefully evaluate these parameters in future outbreaks of filovirus disease.

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Essentials of filoviral load quantification

Abstract

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