Establishing a role for shrimp fortilin in preventing cell death

Potchanapond Graidist, Kenichi Fujise, Warapond Wanna, Kallaya Sritunyalucksana, Amornrat Phongdara

Research output: Contribution to journalArticlepeer-review

34 Scopus citations


White spot syndrome virus (WSSV) is a highly virulent and infectious virus to farmed shrimps and represents a serious threat to aquatic industries. Fortilin, also known as translationally controlled tumor protein (TCTP), protects mammalian cells under stress from cell death. A subtraction hybridization screening in our laboratory between healthy and moribund WSSV-infected Penaeus mondon shrimps has identified a fortilin gene whose messages critically decrease during the terminal stage of the WSSV-induced illness. Fortilin/TCTPs are highly conserved throughout the animal and plant kingdoms, and shrimp fortilin has a 64% identity in amino acid composition with human fortilin. In our previous work, the data clearly suggested that fortilin in shrimp protected WSSV-infected shrimps from death. Although human fortilin has a role in apoptosis regulation, it is not known if shrimp fortilin has any role in apoptosis regulation. We report that fortilin is greatly upregulated in shrimp haemolymph during the early phase of WSSV infection and that its expression abruptly decreases as the shrimp becomes moribund. Strikingly, shrimp fortilin, when overexpressed in mammalian cells, protected them from cell death induced by etoposide, staurosporine, cisplatin, hydroxyurea, and 5-fluorouracil (5-FU). These data suggest that shrimp fortilin, like mammalian fortilin, can protect cells under toxic conditions from death. In addition, since shrimp fortilin was capable of protecting cells in a mammalian environment, this indicates that shrimp and human fortilin use a common cellular pathway to achieve this. Shrimp fortilin may play a critical role in their response to WSSV-infection, through regulation of a cell death pathway that is common to shrimp and humans.

Original languageEnglish (US)
Pages (from-to)157-164
Number of pages8
Issue number1-4
StatePublished - May 31 2006


  • Anti-apoptotic protein
  • Fortilin
  • Penaeus monodon (Pm)
  • Translationally controlled tumor protein (TCTP)
  • White spot syndrome virus (WSSV)

ASJC Scopus subject areas

  • Aquatic Science


Dive into the research topics of 'Establishing a role for shrimp fortilin in preventing cell death'. Together they form a unique fingerprint.

Cite this