Establishing a role for shrimp fortilin in preventing cell death

Potchanapond Graidist, Kenichi Fujise, Warapond Wanna, Kallaya Sritunyalucksana, Amornrat Phongdara

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

White spot syndrome virus (WSSV) is a highly virulent and infectious virus to farmed shrimps and represents a serious threat to aquatic industries. Fortilin, also known as translationally controlled tumor protein (TCTP), protects mammalian cells under stress from cell death. A subtraction hybridization screening in our laboratory between healthy and moribund WSSV-infected Penaeus mondon shrimps has identified a fortilin gene whose messages critically decrease during the terminal stage of the WSSV-induced illness. Fortilin/TCTPs are highly conserved throughout the animal and plant kingdoms, and shrimp fortilin has a 64% identity in amino acid composition with human fortilin. In our previous work, the data clearly suggested that fortilin in shrimp protected WSSV-infected shrimps from death. Although human fortilin has a role in apoptosis regulation, it is not known if shrimp fortilin has any role in apoptosis regulation. We report that fortilin is greatly upregulated in shrimp haemolymph during the early phase of WSSV infection and that its expression abruptly decreases as the shrimp becomes moribund. Strikingly, shrimp fortilin, when overexpressed in mammalian cells, protected them from cell death induced by etoposide, staurosporine, cisplatin, hydroxyurea, and 5-fluorouracil (5-FU). These data suggest that shrimp fortilin, like mammalian fortilin, can protect cells under toxic conditions from death. In addition, since shrimp fortilin was capable of protecting cells in a mammalian environment, this indicates that shrimp and human fortilin use a common cellular pathway to achieve this. Shrimp fortilin may play a critical role in their response to WSSV-infection, through regulation of a cell death pathway that is common to shrimp and humans.

Original languageEnglish (US)
Pages (from-to)157-164
Number of pages8
JournalAquaculture
Volume255
Issue number1-4
DOIs
StatePublished - May 31 2006
Externally publishedYes

Fingerprint

cell death
virus
shrimp
White spot syndrome virus
apoptosis
tumor
amino acid
Penaeus
cells
death
hydroxyurea
fluorouracil
cisplatin
protein
gene
animal
industry
amino acid composition
infection
hemolymph

Keywords

  • Anti-apoptotic protein
  • Fortilin
  • Penaeus monodon (Pm)
  • Translationally controlled tumor protein (TCTP)
  • White spot syndrome virus (WSSV)

ASJC Scopus subject areas

  • Aquatic Science

Cite this

Graidist, P., Fujise, K., Wanna, W., Sritunyalucksana, K., & Phongdara, A. (2006). Establishing a role for shrimp fortilin in preventing cell death. Aquaculture, 255(1-4), 157-164. https://doi.org/10.1016/j.aquaculture.2005.12.023

Establishing a role for shrimp fortilin in preventing cell death. / Graidist, Potchanapond; Fujise, Kenichi; Wanna, Warapond; Sritunyalucksana, Kallaya; Phongdara, Amornrat.

In: Aquaculture, Vol. 255, No. 1-4, 31.05.2006, p. 157-164.

Research output: Contribution to journalArticle

Graidist, P, Fujise, K, Wanna, W, Sritunyalucksana, K & Phongdara, A 2006, 'Establishing a role for shrimp fortilin in preventing cell death', Aquaculture, vol. 255, no. 1-4, pp. 157-164. https://doi.org/10.1016/j.aquaculture.2005.12.023
Graidist, Potchanapond ; Fujise, Kenichi ; Wanna, Warapond ; Sritunyalucksana, Kallaya ; Phongdara, Amornrat. / Establishing a role for shrimp fortilin in preventing cell death. In: Aquaculture. 2006 ; Vol. 255, No. 1-4. pp. 157-164.
@article{50b142ac5f9441ce9e380b1b838e410d,
title = "Establishing a role for shrimp fortilin in preventing cell death",
abstract = "White spot syndrome virus (WSSV) is a highly virulent and infectious virus to farmed shrimps and represents a serious threat to aquatic industries. Fortilin, also known as translationally controlled tumor protein (TCTP), protects mammalian cells under stress from cell death. A subtraction hybridization screening in our laboratory between healthy and moribund WSSV-infected Penaeus mondon shrimps has identified a fortilin gene whose messages critically decrease during the terminal stage of the WSSV-induced illness. Fortilin/TCTPs are highly conserved throughout the animal and plant kingdoms, and shrimp fortilin has a 64{\%} identity in amino acid composition with human fortilin. In our previous work, the data clearly suggested that fortilin in shrimp protected WSSV-infected shrimps from death. Although human fortilin has a role in apoptosis regulation, it is not known if shrimp fortilin has any role in apoptosis regulation. We report that fortilin is greatly upregulated in shrimp haemolymph during the early phase of WSSV infection and that its expression abruptly decreases as the shrimp becomes moribund. Strikingly, shrimp fortilin, when overexpressed in mammalian cells, protected them from cell death induced by etoposide, staurosporine, cisplatin, hydroxyurea, and 5-fluorouracil (5-FU). These data suggest that shrimp fortilin, like mammalian fortilin, can protect cells under toxic conditions from death. In addition, since shrimp fortilin was capable of protecting cells in a mammalian environment, this indicates that shrimp and human fortilin use a common cellular pathway to achieve this. Shrimp fortilin may play a critical role in their response to WSSV-infection, through regulation of a cell death pathway that is common to shrimp and humans.",
keywords = "Anti-apoptotic protein, Fortilin, Penaeus monodon (Pm), Translationally controlled tumor protein (TCTP), White spot syndrome virus (WSSV)",
author = "Potchanapond Graidist and Kenichi Fujise and Warapond Wanna and Kallaya Sritunyalucksana and Amornrat Phongdara",
year = "2006",
month = "5",
day = "31",
doi = "10.1016/j.aquaculture.2005.12.023",
language = "English (US)",
volume = "255",
pages = "157--164",
journal = "Aquaculture",
issn = "0044-8486",
publisher = "Elsevier",
number = "1-4",

}

TY - JOUR

T1 - Establishing a role for shrimp fortilin in preventing cell death

AU - Graidist, Potchanapond

AU - Fujise, Kenichi

AU - Wanna, Warapond

AU - Sritunyalucksana, Kallaya

AU - Phongdara, Amornrat

PY - 2006/5/31

Y1 - 2006/5/31

N2 - White spot syndrome virus (WSSV) is a highly virulent and infectious virus to farmed shrimps and represents a serious threat to aquatic industries. Fortilin, also known as translationally controlled tumor protein (TCTP), protects mammalian cells under stress from cell death. A subtraction hybridization screening in our laboratory between healthy and moribund WSSV-infected Penaeus mondon shrimps has identified a fortilin gene whose messages critically decrease during the terminal stage of the WSSV-induced illness. Fortilin/TCTPs are highly conserved throughout the animal and plant kingdoms, and shrimp fortilin has a 64% identity in amino acid composition with human fortilin. In our previous work, the data clearly suggested that fortilin in shrimp protected WSSV-infected shrimps from death. Although human fortilin has a role in apoptosis regulation, it is not known if shrimp fortilin has any role in apoptosis regulation. We report that fortilin is greatly upregulated in shrimp haemolymph during the early phase of WSSV infection and that its expression abruptly decreases as the shrimp becomes moribund. Strikingly, shrimp fortilin, when overexpressed in mammalian cells, protected them from cell death induced by etoposide, staurosporine, cisplatin, hydroxyurea, and 5-fluorouracil (5-FU). These data suggest that shrimp fortilin, like mammalian fortilin, can protect cells under toxic conditions from death. In addition, since shrimp fortilin was capable of protecting cells in a mammalian environment, this indicates that shrimp and human fortilin use a common cellular pathway to achieve this. Shrimp fortilin may play a critical role in their response to WSSV-infection, through regulation of a cell death pathway that is common to shrimp and humans.

AB - White spot syndrome virus (WSSV) is a highly virulent and infectious virus to farmed shrimps and represents a serious threat to aquatic industries. Fortilin, also known as translationally controlled tumor protein (TCTP), protects mammalian cells under stress from cell death. A subtraction hybridization screening in our laboratory between healthy and moribund WSSV-infected Penaeus mondon shrimps has identified a fortilin gene whose messages critically decrease during the terminal stage of the WSSV-induced illness. Fortilin/TCTPs are highly conserved throughout the animal and plant kingdoms, and shrimp fortilin has a 64% identity in amino acid composition with human fortilin. In our previous work, the data clearly suggested that fortilin in shrimp protected WSSV-infected shrimps from death. Although human fortilin has a role in apoptosis regulation, it is not known if shrimp fortilin has any role in apoptosis regulation. We report that fortilin is greatly upregulated in shrimp haemolymph during the early phase of WSSV infection and that its expression abruptly decreases as the shrimp becomes moribund. Strikingly, shrimp fortilin, when overexpressed in mammalian cells, protected them from cell death induced by etoposide, staurosporine, cisplatin, hydroxyurea, and 5-fluorouracil (5-FU). These data suggest that shrimp fortilin, like mammalian fortilin, can protect cells under toxic conditions from death. In addition, since shrimp fortilin was capable of protecting cells in a mammalian environment, this indicates that shrimp and human fortilin use a common cellular pathway to achieve this. Shrimp fortilin may play a critical role in their response to WSSV-infection, through regulation of a cell death pathway that is common to shrimp and humans.

KW - Anti-apoptotic protein

KW - Fortilin

KW - Penaeus monodon (Pm)

KW - Translationally controlled tumor protein (TCTP)

KW - White spot syndrome virus (WSSV)

UR - http://www.scopus.com/inward/record.url?scp=33747299820&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=33747299820&partnerID=8YFLogxK

U2 - 10.1016/j.aquaculture.2005.12.023

DO - 10.1016/j.aquaculture.2005.12.023

M3 - Article

VL - 255

SP - 157

EP - 164

JO - Aquaculture

JF - Aquaculture

SN - 0044-8486

IS - 1-4

ER -