Establishment and characterization of a human carcinoid in nude mice and effect of various agents on tumor growth

B. Mark Evers, Courtney Townsend, J. Robert Upp, Erick Allen, Stephen C. Hurlbut, Sun Whe Kim, Srinivasan Rajaraman, Pomila Singh, Jean Claude Reubi, James C. Thompson

Research output: Contribution to journalArticle

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Abstract

The authors have established a long-term tissue culture cell line (BON) derived from a metastatic human carcinoid tumor of the pancreas. The cells have been in continuous passage for 46 months. Tissue culture cells produce tumors in a dosedependent fashion after SC inoculation of cell suspensions in athymic nude mice. BON tumors, grown in nude mice, are histologically identical to the original tumor; they possess gastrin and somatostatin receptors, synthesize serotonin and chromogranin A, and have a doubling time of approximately 13 days. The antiproliferative effects of the long-acting somatostatin analogue, SMS 201-995 (300 μg/kg, t.i.d.), and 2% α-difluoromethylornithine on BON xenografts in nude mice were examined. Tumor size was significantly decreased by day 14 of treatment with either agent and at all points of analysis thereafter until the animals were killed (day 33). In addition, tumor weight, DNA, RNA, and protein contents were significantly decreased in treated mice compared with controls. Establishment of this human carcinoid xenograft line, BON, provides an excellent model to study further the biological behavior of carcinoid tumors and the in vivo effect of chemotherapeutic agents on tumor growth.

Original languageEnglish (US)
Pages (from-to)303-311
Number of pages9
JournalGastroenterology
Volume101
Issue number2
StatePublished - 1991

Fingerprint

Carcinoid Tumor
Nude Mice
Growth
Neoplasms
Heterografts
Cholecystokinin B Receptor
Eflornithine
Chromogranin A
Somatostatin Receptors
Octreotide
Somatostatin
Tumor Burden
Pancreas
Serotonin
Suspensions
Cell Culture Techniques
RNA
Cell Line
DNA
Proteins

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Evers, B. M., Townsend, C., Upp, J. R., Allen, E., Hurlbut, S. C., Kim, S. W., ... Thompson, J. C. (1991). Establishment and characterization of a human carcinoid in nude mice and effect of various agents on tumor growth. Gastroenterology, 101(2), 303-311.

Establishment and characterization of a human carcinoid in nude mice and effect of various agents on tumor growth. / Evers, B. Mark; Townsend, Courtney; Upp, J. Robert; Allen, Erick; Hurlbut, Stephen C.; Kim, Sun Whe; Rajaraman, Srinivasan; Singh, Pomila; Reubi, Jean Claude; Thompson, James C.

In: Gastroenterology, Vol. 101, No. 2, 1991, p. 303-311.

Research output: Contribution to journalArticle

Evers, BM, Townsend, C, Upp, JR, Allen, E, Hurlbut, SC, Kim, SW, Rajaraman, S, Singh, P, Reubi, JC & Thompson, JC 1991, 'Establishment and characterization of a human carcinoid in nude mice and effect of various agents on tumor growth', Gastroenterology, vol. 101, no. 2, pp. 303-311.
Evers, B. Mark ; Townsend, Courtney ; Upp, J. Robert ; Allen, Erick ; Hurlbut, Stephen C. ; Kim, Sun Whe ; Rajaraman, Srinivasan ; Singh, Pomila ; Reubi, Jean Claude ; Thompson, James C. / Establishment and characterization of a human carcinoid in nude mice and effect of various agents on tumor growth. In: Gastroenterology. 1991 ; Vol. 101, No. 2. pp. 303-311.
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