Estimating bacteria diversity in different organs of nine species of mosquito by next generation sequencing

M. V. Mancini, C. Damiani, A. Accoti, M. Tallarita, E. Nunzi, A. Cappelli, J. Bozic, R. Catanzani, P. Rossi, M. Valzano, A. Serrao, I. Ricci, R. Spaccapelo, G. Favia

Research output: Contribution to journalArticlepeer-review

71 Scopus citations

Abstract

Background: Symbiosis in insects is accumulating significant amount of studies: the description of a wide array of mutualistic associations across the evolutionary history of insects suggests that resident microbiota acts as a driving force by affecting several aspects of hosts biology. Among arthropods, mosquito midgut microbiota has been largely investigated, providing crucial insights on the role and implications of host-symbiont relationships. However, limited amount of studies addressed their efforts on the investigation of microbiota colonizing salivary glands and reproductive tracts, crucial organs for pathogen invasion and vertical transmission of symbiotic microorganisms. Using 16S rRNA gene sequencing-based approach, we analysed the microbiota of gut, salivary glands and reproductive tracts of several mosquito species, representing some of the main vectors of diseases, aiming at describing the dynamics of bacterial communities within the individual. Results: We identified a shared core microbiota between different mosquito species, although interesting inter- and intra-species differences were detected. Additionally, our results showed deep divergences between genera, underlining microbiota specificity and adaptation to their host. Conclusions: The comprehensive landscape of the bacterial microbiota components may ultimately provide crucial insights and novel targets for possible application of symbionts in innovative strategies for the control of vector borne diseases, globally named Symbiotic Control (SC), and suggesting that the holobiont of different mosquito species may significantly vary. Moreover, mosquito species are characterized by distinctive microbiota in different organs, likely reflecting different functions and/or adaptation processes.

Original languageEnglish (US)
Article number126
JournalBMC Microbiology
Volume18
Issue number1
DOIs
StatePublished - Oct 4 2018
Externally publishedYes

Keywords

  • Metagenomics
  • Mosquitoes
  • Symbionts

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

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