Estrogen Receptor Characterization in a Transplantable Mouse Mammary Tumor

Watson Cheryl, Daniel Medina, James H. Clark

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The estrogen receptor was characterized in a new transplantable Mammary tumor line (MXT) which was induced by urethan treatment in C57BL x DBAf F1 mice. the tumor is a ductal papillary carcinoma that is ovarian dependent. Treatment of ovariectomized mice with estradiol benzoate results in rapid growth of the tumor. Cytoplasmic and nuclear-bound estrogen receptors were measured by the charcoal adsorption exchange Assay and by the [3H]estradiol exchange Assay, respectively. Tumor cytosol contained relatively large quantities of receptors, 8 to 9 fmoles/mg wet weight, which were depleted from the cytoplasmic compartment after an injection of 2 μg of estradiol. This depletion was accompanied by concomitant and stoichiometric accumulation of receptors by the nuclear fraction. Thus an apparent cytoplasmic-to-nuclear translocation was demonstrated. the affinity of both cytoplasmic and nuclear receptors was similar to that reported by othrs (~ 1 nM). Receptor binding was specific for estrogens, and Other steroids showed no competitive inhibition at concentrations up to 10-7M. these results demonstrate that this tumor line contains relatively large quantities of estrogen receptors that are similar to those found in normal Tissue. This finding in a tumor that is transplantable makes it an ideal model system for the study of growth control by estrogens in neoplastic Tissue.

Original languageEnglish (US)
Pages (from-to)3344-3348
Number of pages5
JournalCancer Research
Volume37
Issue number9
StatePublished - 1977
Externally publishedYes

Fingerprint

Estrogen Receptors
Breast Neoplasms
Neoplasms
Cytoplasmic and Nuclear Receptors
Estradiol
Estrogens
Ductal Carcinoma
Papillary Carcinoma
Charcoal
Urethane
Growth
Cytosol
Adsorption
Steroids
Weights and Measures
Injections
Therapeutics

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Cheryl, W., Medina, D., & Clark, J. H. (1977). Estrogen Receptor Characterization in a Transplantable Mouse Mammary Tumor. Cancer Research, 37(9), 3344-3348.

Estrogen Receptor Characterization in a Transplantable Mouse Mammary Tumor. / Cheryl, Watson; Medina, Daniel; Clark, James H.

In: Cancer Research, Vol. 37, No. 9, 1977, p. 3344-3348.

Research output: Contribution to journalArticle

Cheryl, W, Medina, D & Clark, JH 1977, 'Estrogen Receptor Characterization in a Transplantable Mouse Mammary Tumor', Cancer Research, vol. 37, no. 9, pp. 3344-3348.
Cheryl, Watson ; Medina, Daniel ; Clark, James H. / Estrogen Receptor Characterization in a Transplantable Mouse Mammary Tumor. In: Cancer Research. 1977 ; Vol. 37, No. 9. pp. 3344-3348.
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