Eukaryotic translesion synthesis DNA polymerases

Specificity of structure and function

Satya Prakash, Robert E. Johnson, Louise Prakash

Research output: Contribution to journalArticle

732 Citations (Scopus)

Abstract

This review focuses on eukaryotic translesion synthesis (TLS) DNA polymerases, and the emphasis is on Saccharomyces cerevisiae and human Y-family polymerases (Pols) η, ι, κ, and Rev 1, as well as on Polζ, which is a member of the B-family polymerases. The fidelity, mismatch extension ability, and lesion bypass efficiencies of these different polymerases are examined and evaluated in the context of their structures. One major conclusion is that, despite the overall similarity of basic structural features among the Y-family polymerases, there is a high degree of specificity in their lesion bypass properties. Some are able to bypass a particular DNA lesion, whereas others are efficient at only the insertion step or the extension step of lesion bypass. This functional divergence is related to the differences in their structures. Polζ is a highly specialized polymerase specifically adapted for extending primer termini opposite from a diverse array of DNA lesions, and depending upon the DNA lesion, it contributes to lesion bypass in a mutagenic or in an error-free manner. Proliferating cell nuclear antigen (PCNA) provides the central scaffold to which TLS polymerases bind for access to the replication ensemble stalled at a lesion site, and Rad6-Rad18-dependent protein ubiquitination is important for polymerase exchange.

Original languageEnglish (US)
Pages (from-to)317-353
Number of pages37
JournalAnnual Review of Biochemistry
Volume74
DOIs
StatePublished - 2005

Fingerprint

DNA-Directed DNA Polymerase
DNA
Aptitude
Ubiquitination
Proliferating Cell Nuclear Antigen
Oligonucleotide Array Sequence Analysis
Scaffolds
Yeast
Saccharomyces cerevisiae
Efficiency
Proteins

Keywords

  • DNA polymerase structures
  • Lesion bypass
  • Rad6-Rad18 enzyme complex
  • Y-family DNA polymerases

ASJC Scopus subject areas

  • Biochemistry

Cite this

Eukaryotic translesion synthesis DNA polymerases : Specificity of structure and function. / Prakash, Satya; Johnson, Robert E.; Prakash, Louise.

In: Annual Review of Biochemistry, Vol. 74, 2005, p. 317-353.

Research output: Contribution to journalArticle

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