Evaluating the role of viral proteins in HIV-mediated neurotoxicity using primary human neuronal cultures

Vasudev R. Rao; Eliseo Eugenin; Vinayaka R. Prasad

doi:10.1007/978-1-4939-3046-3_25

Evaluating the role of viral proteins in HIV-mediated neurotoxicity using primary human neuronal cultures

Vasudev R. Rao, Eliseo Eugenin, Vinayaka R. Prasad

Research output: Chapter in Book/Report/Conference proceeding › Chapter

3 Scopus citations

Abstract

Despite the inability of HIV-1 to infect neurons, over half of the HIV-1-infected population in the USA suffers from neurocognitive dysfunction. HIV-infected immune cells in the periphery enter the central nervous system by causing a breach in the blood-brain barrier. The damage to the neurons is mediated by viral and host toxic products released by activated and infected immune and glial cells. To evaluate the toxicity of any viral isolate, viral protein, or host inflammatory protein, we describe a protocol to assess the neuronal apoptosis and synaptic compromise in primary cultures of human neurons and astrocytes.

Original language	English (US)
Title of host publication	Methods in Molecular Biology
Publisher	Humana Press Inc.
Pages	367-376
Number of pages	10
DOIs	https://doi.org/10.1007/978-1-4939-3046-3_25
State	Published - Jan 1 2016
Externally published	Yes

Publication series

Name	Methods in Molecular Biology
Volume	1354
ISSN (Print)	1064-3745

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Keywords

Chemokines
Cytokines
Gp120
HAD
HAND
HIV-1
HIV-associated dementia
HIV-associated neurocognitive dysfunction
Neuro-inflammation
Neuronal apoptosis
Neuronal damage
Tat

ASJC Scopus subject areas

Molecular Biology
Genetics

Cite this

Rao, V. R., Eugenin, E., & Prasad, V. R. (2016). Evaluating the role of viral proteins in HIV-mediated neurotoxicity using primary human neuronal cultures. In Methods in Molecular Biology (pp. 367-376). (Methods in Molecular Biology; Vol. 1354). Humana Press Inc.. https://doi.org/10.1007/978-1-4939-3046-3_25

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Access to Document

10.1007/978-1-4939-3046-3_25