TY - JOUR
T1 - Evaluating the use of fluorescence-based flow cytometry assay for dengue diagnosis using peripheral blood mononuclear cells
AU - Barreira, Luzia Aparecida Costa
AU - Scheucher, Priscila Santos
AU - Romeiro, Marilia Farignoli
AU - La Serra, Leonardo
AU - Badra, Soraya Jabur
AU - De Souza, William Marciel
AU - Figueiredo, Luiz Tadeu Moraes
N1 - Publisher Copyright:
© 2018, Sociedade Brasileira de Medicina Tropical. All rights reserved.
PY - 2018/3/1
Y1 - 2018/3/1
N2 - Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). Results: All three assays identified 29.1% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.
AB - Introduction: Dengue virus (DENV) is the most important arthropod-borne viral disease worldwide with an estimated 50 million infections occurring each year. Methods: In this study, we present a flow cytometry assay (FACS) for diagnosing DENV, and compare its results with those of the non-structural protein 1 (NS1) immunochromatographic assay and reverse transcriptase polymerase chain reaction (RT-PCR). Results: All three assays identified 29.1% (39/134) of the patients as denguepositive. The FACS approach and real-time RT-PCR detected the DENV in 39 and 44 samples, respectively. On the other hand, the immunochromatographic assay detected the NS1 protein in 40.1% (56/134) of the patients. The Cohen's kappa coefficient analysis revealed a substantial agreement among the three methods. Conclusions: The FACS approach may be a useful alternative for dengue diagnosis and can be implemented in public and private laboratories.
KW - Arbovirus
KW - Dengue virus
KW - Flavivirus
KW - Flow cytometry
KW - Viral diagnostic
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U2 - 10.1590/0037-8682-0404-2017
DO - 10.1590/0037-8682-0404-2017
M3 - Article
C2 - 29768549
AN - SCOPUS:85047004975
SN - 0037-8682
VL - 51
SP - 168
EP - 173
JO - Revista da Sociedade Brasileira de Medicina Tropical
JF - Revista da Sociedade Brasileira de Medicina Tropical
IS - 2
ER -