Evaluation of a commercially-available surrogate virus neutralization test for severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2)

Emelissa J. Valcourt, Kathy Manguiat, Alyssia Robinson, Julie Chih Yu Chen, Kristina Dimitrova, Clark Philipson, Lise Lamoureux, Elizabeth McLachlan, Zachary Schiffman, Michael A. Drebot, Heidi Wood

Research output: Contribution to journalArticlepeer-review

72 Scopus citations

Abstract

There remains an urgent need for assays to quantify humoral protective immunity to SARS-CoV-2 to understand the immune responses of COVID-19 patients, evaluate efficacy of vaccine candidates in clinical trials, and conduct large-scale epidemiological studies. The plaque-reduction neutralization test (PRNT) is the reference-standard for quantifying antibodies capable of neutralizing SARS-CoV-2. However, the PRNT is logistically demanding, time-consuming, and requires containment level-3 facilities to safely work with live virus. In contrast, a surrogate virus neutralization test (sVNT) manufactured by Genscript is a quick and simple assay that detects antibodies that inhibit the RBD-ACE2 interaction, crucial for virus entry into host cells. In this study, we evaluate the sensitivity, specificity, and cross-reactivity of the sVNT compared with the PRNT using both 50% and 90% SARS-CoV-2 neutralization as a reference-standard. We found that the sVNT provides a high-throughput screening tool prior to confirmatory PRNT testing for the evaluation of SARS-CoV-2 neutralizing antibodies.

Original languageEnglish (US)
Article number115294
JournalDiagnostic Microbiology and Infectious Disease
Volume99
Issue number4
DOIs
StatePublished - Apr 2021
Externally publishedYes

Keywords

  • COVID-19
  • Immunity
  • Neutralization test

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

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