Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2 in the setting of multiple transmitted viral subtypes

S. J. Reynolds, L. M. Ndongala, C. C. Luo, K. Mwandagalirwa, A. J. Losoma, K. J. Mwamba, E. Bazepeyo, N. E. Nzilambi, T. C. Quinn, R. C. Bollinger

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

Rapid HIV testing is an appealing strategy in the approach to HIV diagnosis in developing countries. Concern has been raised about the use of these tests in the setting of multiply transmitted HIV subtypes. We sought to compare the OraQuick™ HIV-1/2 Test, a qualitative immunochromatographic test for the detection of antibodies to HIV-1 and HIV-2 using stored sera, with a conventional enzyme immunoassay (EIA)/Western blot (WB) algorithm. The study design used was a blinded retrospective study. Samples were collected on patients attending sexually transmitted disease clinics and tuberculosis clinics in Kinshasa, the Democratic Republic of Congo and included 72 known HIV seropositive and 131 known HIV seronegative subjects. All 72 known HIV seropositive samples were positive by OraQuick and all 131 known HIV seronegative samples were negative by OraQuick resulting in 100% sensitivity and specificity. We conclude that the OraQuick rapid HIV-1/2 test performs well in the setting of diverse HIV viral subtypes.

Original languageEnglish (US)
Pages (from-to)171-173
Number of pages3
JournalInternational Journal of STD and AIDS
Volume13
Issue number3
DOIs
StatePublished - 2002
Externally publishedYes

Fingerprint

HIV-2
HIV-1
HIV
Antibodies
Democratic Republic of the Congo
Sexually Transmitted Diseases
Immunoenzyme Techniques
Developing Countries
Tuberculosis
Retrospective Studies
Western Blotting
Sensitivity and Specificity
Serum

Keywords

  • HIV diagnostic tests
  • HIV subtypes

ASJC Scopus subject areas

  • Dermatology
  • Public Health, Environmental and Occupational Health
  • Microbiology (medical)
  • Immunology

Cite this

Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2 in the setting of multiple transmitted viral subtypes. / Reynolds, S. J.; Ndongala, L. M.; Luo, C. C.; Mwandagalirwa, K.; Losoma, A. J.; Mwamba, K. J.; Bazepeyo, E.; Nzilambi, N. E.; Quinn, T. C.; Bollinger, R. C.

In: International Journal of STD and AIDS, Vol. 13, No. 3, 2002, p. 171-173.

Research output: Contribution to journalArticle

Reynolds, SJ, Ndongala, LM, Luo, CC, Mwandagalirwa, K, Losoma, AJ, Mwamba, KJ, Bazepeyo, E, Nzilambi, NE, Quinn, TC & Bollinger, RC 2002, 'Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2 in the setting of multiple transmitted viral subtypes', International Journal of STD and AIDS, vol. 13, no. 3, pp. 171-173. https://doi.org/10.1258/0956462021924857
Reynolds, S. J. ; Ndongala, L. M. ; Luo, C. C. ; Mwandagalirwa, K. ; Losoma, A. J. ; Mwamba, K. J. ; Bazepeyo, E. ; Nzilambi, N. E. ; Quinn, T. C. ; Bollinger, R. C. / Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2 in the setting of multiple transmitted viral subtypes. In: International Journal of STD and AIDS. 2002 ; Vol. 13, No. 3. pp. 171-173.
@article{cc3813377f34427db035fa2cf727884a,
title = "Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2 in the setting of multiple transmitted viral subtypes",
abstract = "Rapid HIV testing is an appealing strategy in the approach to HIV diagnosis in developing countries. Concern has been raised about the use of these tests in the setting of multiply transmitted HIV subtypes. We sought to compare the OraQuick™ HIV-1/2 Test, a qualitative immunochromatographic test for the detection of antibodies to HIV-1 and HIV-2 using stored sera, with a conventional enzyme immunoassay (EIA)/Western blot (WB) algorithm. The study design used was a blinded retrospective study. Samples were collected on patients attending sexually transmitted disease clinics and tuberculosis clinics in Kinshasa, the Democratic Republic of Congo and included 72 known HIV seropositive and 131 known HIV seronegative subjects. All 72 known HIV seropositive samples were positive by OraQuick and all 131 known HIV seronegative samples were negative by OraQuick resulting in 100{\%} sensitivity and specificity. We conclude that the OraQuick rapid HIV-1/2 test performs well in the setting of diverse HIV viral subtypes.",
keywords = "HIV diagnostic tests, HIV subtypes",
author = "Reynolds, {S. J.} and Ndongala, {L. M.} and Luo, {C. C.} and K. Mwandagalirwa and Losoma, {A. J.} and Mwamba, {K. J.} and E. Bazepeyo and Nzilambi, {N. E.} and Quinn, {T. C.} and Bollinger, {R. C.}",
year = "2002",
doi = "10.1258/0956462021924857",
language = "English (US)",
volume = "13",
pages = "171--173",
journal = "International Journal of STD and AIDS",
issn = "0956-4624",
publisher = "SAGE Publications Ltd",
number = "3",

}

TY - JOUR

T1 - Evaluation of a rapid test for the detection of antibodies to human immunodeficiency virus type 1 and 2 in the setting of multiple transmitted viral subtypes

AU - Reynolds, S. J.

AU - Ndongala, L. M.

AU - Luo, C. C.

AU - Mwandagalirwa, K.

AU - Losoma, A. J.

AU - Mwamba, K. J.

AU - Bazepeyo, E.

AU - Nzilambi, N. E.

AU - Quinn, T. C.

AU - Bollinger, R. C.

PY - 2002

Y1 - 2002

N2 - Rapid HIV testing is an appealing strategy in the approach to HIV diagnosis in developing countries. Concern has been raised about the use of these tests in the setting of multiply transmitted HIV subtypes. We sought to compare the OraQuick™ HIV-1/2 Test, a qualitative immunochromatographic test for the detection of antibodies to HIV-1 and HIV-2 using stored sera, with a conventional enzyme immunoassay (EIA)/Western blot (WB) algorithm. The study design used was a blinded retrospective study. Samples were collected on patients attending sexually transmitted disease clinics and tuberculosis clinics in Kinshasa, the Democratic Republic of Congo and included 72 known HIV seropositive and 131 known HIV seronegative subjects. All 72 known HIV seropositive samples were positive by OraQuick and all 131 known HIV seronegative samples were negative by OraQuick resulting in 100% sensitivity and specificity. We conclude that the OraQuick rapid HIV-1/2 test performs well in the setting of diverse HIV viral subtypes.

AB - Rapid HIV testing is an appealing strategy in the approach to HIV diagnosis in developing countries. Concern has been raised about the use of these tests in the setting of multiply transmitted HIV subtypes. We sought to compare the OraQuick™ HIV-1/2 Test, a qualitative immunochromatographic test for the detection of antibodies to HIV-1 and HIV-2 using stored sera, with a conventional enzyme immunoassay (EIA)/Western blot (WB) algorithm. The study design used was a blinded retrospective study. Samples were collected on patients attending sexually transmitted disease clinics and tuberculosis clinics in Kinshasa, the Democratic Republic of Congo and included 72 known HIV seropositive and 131 known HIV seronegative subjects. All 72 known HIV seropositive samples were positive by OraQuick and all 131 known HIV seronegative samples were negative by OraQuick resulting in 100% sensitivity and specificity. We conclude that the OraQuick rapid HIV-1/2 test performs well in the setting of diverse HIV viral subtypes.

KW - HIV diagnostic tests

KW - HIV subtypes

UR - http://www.scopus.com/inward/record.url?scp=18244384758&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=18244384758&partnerID=8YFLogxK

U2 - 10.1258/0956462021924857

DO - 10.1258/0956462021924857

M3 - Article

C2 - 11860693

AN - SCOPUS:18244384758

VL - 13

SP - 171

EP - 173

JO - International Journal of STD and AIDS

JF - International Journal of STD and AIDS

SN - 0956-4624

IS - 3

ER -