Evaluation of allergenicity of the extract of Japanese juniper pollen reacting to sera from asthmatic children--by the methods of enzyme-linked immunosorbent assay and immunoblotting

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Abstract

We investigated the allergenicity of pollen extract of Japanese juniper (Juniperus rigida, Cupressaceae family) to sera of 49 asthmatic children by the methods of enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Three bands stained with Coomassie Brilliant Blue R were detected on SDS-PAGE. Sera from 27 (55.1%) out of the 49 children showed positive reaction to the pollen extract in ELISA. The same sera from the 27 children were used as the first antibody in immunoblotting, which confirmed the presence of a band of protein with 70 K dalton molecular weight (M.W) common to the all sera. This band was bound with concanavalin A in lectins. We successfully purified the antigenic substance of Japanese juniper pollen from this band by the electroelution method. The major allergen of Japanese juniper pollen is glycoprotein with 70 KM.W. On sandwich-ELISA, there was no reaction of Sugi Basic Protein (SBP) and anti-SBP to Japanese juniper is an allergen that has no cross-reactivity with SBP.

Original languageEnglish (US)
Pages (from-to)1459-1465
Number of pages7
JournalJapanese Journal of Allergology
Volume41
Issue number10
StatePublished - Oct 1992
Externally publishedYes

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Juniperus
Pollen
Immunoblotting
Enzyme-Linked Immunosorbent Assay
Serum
Allergens
Proteins
Cupressaceae
Concanavalin A
Lectins
Polyacrylamide Gel Electrophoresis
Glycoproteins
Molecular Weight
Antibodies

ASJC Scopus subject areas

  • Immunology and Allergy

Cite this

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title = "Evaluation of allergenicity of the extract of Japanese juniper pollen reacting to sera from asthmatic children--by the methods of enzyme-linked immunosorbent assay and immunoblotting",
abstract = "We investigated the allergenicity of pollen extract of Japanese juniper (Juniperus rigida, Cupressaceae family) to sera of 49 asthmatic children by the methods of enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Three bands stained with Coomassie Brilliant Blue R were detected on SDS-PAGE. Sera from 27 (55.1{\%}) out of the 49 children showed positive reaction to the pollen extract in ELISA. The same sera from the 27 children were used as the first antibody in immunoblotting, which confirmed the presence of a band of protein with 70 K dalton molecular weight (M.W) common to the all sera. This band was bound with concanavalin A in lectins. We successfully purified the antigenic substance of Japanese juniper pollen from this band by the electroelution method. The major allergen of Japanese juniper pollen is glycoprotein with 70 KM.W. On sandwich-ELISA, there was no reaction of Sugi Basic Protein (SBP) and anti-SBP to Japanese juniper is an allergen that has no cross-reactivity with SBP.",
author = "Terumi Midoro-Horiuti",
year = "1992",
month = "10",
language = "English (US)",
volume = "41",
pages = "1459--1465",
journal = "Japanese Journal of Allergology",
issn = "0021-4884",
publisher = "Japanese Society of Allergology",
number = "10",

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AU - Midoro-Horiuti, Terumi

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N2 - We investigated the allergenicity of pollen extract of Japanese juniper (Juniperus rigida, Cupressaceae family) to sera of 49 asthmatic children by the methods of enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Three bands stained with Coomassie Brilliant Blue R were detected on SDS-PAGE. Sera from 27 (55.1%) out of the 49 children showed positive reaction to the pollen extract in ELISA. The same sera from the 27 children were used as the first antibody in immunoblotting, which confirmed the presence of a band of protein with 70 K dalton molecular weight (M.W) common to the all sera. This band was bound with concanavalin A in lectins. We successfully purified the antigenic substance of Japanese juniper pollen from this band by the electroelution method. The major allergen of Japanese juniper pollen is glycoprotein with 70 KM.W. On sandwich-ELISA, there was no reaction of Sugi Basic Protein (SBP) and anti-SBP to Japanese juniper is an allergen that has no cross-reactivity with SBP.

AB - We investigated the allergenicity of pollen extract of Japanese juniper (Juniperus rigida, Cupressaceae family) to sera of 49 asthmatic children by the methods of enzyme-linked immunosorbent assay (ELISA) and immunoblotting. Three bands stained with Coomassie Brilliant Blue R were detected on SDS-PAGE. Sera from 27 (55.1%) out of the 49 children showed positive reaction to the pollen extract in ELISA. The same sera from the 27 children were used as the first antibody in immunoblotting, which confirmed the presence of a band of protein with 70 K dalton molecular weight (M.W) common to the all sera. This band was bound with concanavalin A in lectins. We successfully purified the antigenic substance of Japanese juniper pollen from this band by the electroelution method. The major allergen of Japanese juniper pollen is glycoprotein with 70 KM.W. On sandwich-ELISA, there was no reaction of Sugi Basic Protein (SBP) and anti-SBP to Japanese juniper is an allergen that has no cross-reactivity with SBP.

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