Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats

Milindmitra Lonare, Manoj Kumar, Sachin Raut, Amar More, Sagar Doltade, Prarabdh Badgujar, Avinash Telang

Research output: Contribution to journalArticlepeer-review

66 Scopus citations


This study was undertaken to investigate the toxic effects of imidacloprid (IM) on male reproductive system and ameliorative effect of curcumin (CMN) in male Wistar rats. For this purpose, IM (45 and 90 mg/kg, body weight) and CMN (100 mg/kg, body weight) were administered orally to the rats either alone or in combinations for a period of 28 days. At the end of experiment, male reproductive toxicity parameters (total sperm count and sperm abnormalities), testosterone level, steroidal enzymatic activity [3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD], and oxidative stress indicators were estimated in testis and plasma. IM treatments resulted in significant decrease (p < 0.05) in total epididymal sperm count, sperm motility, live sperm count, and increase (p < 0.05) in sperm abnormalities. Activities of gamma-glutamyl transpeptidase, lactate dehydrogenase-x, and sorbitol dehydrogenase were significantly increased (p < 0.05), while, 3β-HSD and 17β-HSD enzymatic activity along with testosterone concentration in testis and plasma were decreased significantly (p < 0.05) in IM-treated rats. IM exposure resulted in significant increase (p < 0.05) in LPO and decrease (p < 0.05) in GSH level along with decreased activities of CAT, SOD, GPx, and GST. IM-treated rats showed histopathological alterations in testis and epididymis. However, the reproductive toxicity parameters, oxidative stress indicators, and histopathological changes were minimized and functional restorations were noticed by co-administration of CMN in IM-treated rats. The results of this study suggest that IM-induced male reproductive toxic effects could be ameliorated by CMN supplementation.

Original languageEnglish (US)
Pages (from-to)1250-1263
Number of pages14
JournalEnvironmental Toxicology
Issue number10
StatePublished - Oct 1 2016
Externally publishedYes


  • curcumin
  • imidaclorid
  • oxidative stress
  • reproductive toxicity

ASJC Scopus subject areas

  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis


Dive into the research topics of 'Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats'. Together they form a unique fingerprint.

Cite this