Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats

Milindmitra Lonare, Manoj Kumar, Sachin Raut, Amar More, Sagar Doltade, Prarabdh Badgujar, Avinash Telang

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

This study was undertaken to investigate the toxic effects of imidacloprid (IM) on male reproductive system and ameliorative effect of curcumin (CMN) in male Wistar rats. For this purpose, IM (45 and 90 mg/kg, body weight) and CMN (100 mg/kg, body weight) were administered orally to the rats either alone or in combinations for a period of 28 days. At the end of experiment, male reproductive toxicity parameters (total sperm count and sperm abnormalities), testosterone level, steroidal enzymatic activity [3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD], and oxidative stress indicators were estimated in testis and plasma. IM treatments resulted in significant decrease (p < 0.05) in total epididymal sperm count, sperm motility, live sperm count, and increase (p < 0.05) in sperm abnormalities. Activities of gamma-glutamyl transpeptidase, lactate dehydrogenase-x, and sorbitol dehydrogenase were significantly increased (p < 0.05), while, 3β-HSD and 17β-HSD enzymatic activity along with testosterone concentration in testis and plasma were decreased significantly (p < 0.05) in IM-treated rats. IM exposure resulted in significant increase (p < 0.05) in LPO and decrease (p < 0.05) in GSH level along with decreased activities of CAT, SOD, GPx, and GST. IM-treated rats showed histopathological alterations in testis and epididymis. However, the reproductive toxicity parameters, oxidative stress indicators, and histopathological changes were minimized and functional restorations were noticed by co-administration of CMN in IM-treated rats. The results of this study suggest that IM-induced male reproductive toxic effects could be ameliorated by CMN supplementation.

Original languageEnglish (US)
Pages (from-to)1250-1263
Number of pages14
JournalEnvironmental Toxicology
Volume31
Issue number10
DOIs
StatePublished - Oct 1 2016
Externally publishedYes

Fingerprint

imidacloprid
Curcumin
Toxicity
Wistar Rats
Rats
sperm
toxicity
Sperm Count
3-Hydroxysteroid Dehydrogenases
Testis
Oxidative stress
Poisons
testosterone
abnormality
Testosterone
Spermatozoa
Oxidative Stress
Body Weight
L-Iditol 2-Dehydrogenase
Plasmas

Keywords

  • curcumin
  • imidaclorid
  • oxidative stress
  • reproductive toxicity

ASJC Scopus subject areas

  • Medicine(all)
  • Toxicology
  • Management, Monitoring, Policy and Law
  • Health, Toxicology and Mutagenesis

Cite this

Lonare, M., Kumar, M., Raut, S., More, A., Doltade, S., Badgujar, P., & Telang, A. (2016). Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats. Environmental Toxicology, 31(10), 1250-1263. https://doi.org/10.1002/tox.22132

Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats. / Lonare, Milindmitra; Kumar, Manoj; Raut, Sachin; More, Amar; Doltade, Sagar; Badgujar, Prarabdh; Telang, Avinash.

In: Environmental Toxicology, Vol. 31, No. 10, 01.10.2016, p. 1250-1263.

Research output: Contribution to journalArticle

Lonare, M, Kumar, M, Raut, S, More, A, Doltade, S, Badgujar, P & Telang, A 2016, 'Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats', Environmental Toxicology, vol. 31, no. 10, pp. 1250-1263. https://doi.org/10.1002/tox.22132
Lonare, Milindmitra ; Kumar, Manoj ; Raut, Sachin ; More, Amar ; Doltade, Sagar ; Badgujar, Prarabdh ; Telang, Avinash. / Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats. In: Environmental Toxicology. 2016 ; Vol. 31, No. 10. pp. 1250-1263.
@article{f11c84ecc58548c39021d4ae4e7258ab,
title = "Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats",
abstract = "This study was undertaken to investigate the toxic effects of imidacloprid (IM) on male reproductive system and ameliorative effect of curcumin (CMN) in male Wistar rats. For this purpose, IM (45 and 90 mg/kg, body weight) and CMN (100 mg/kg, body weight) were administered orally to the rats either alone or in combinations for a period of 28 days. At the end of experiment, male reproductive toxicity parameters (total sperm count and sperm abnormalities), testosterone level, steroidal enzymatic activity [3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD], and oxidative stress indicators were estimated in testis and plasma. IM treatments resulted in significant decrease (p < 0.05) in total epididymal sperm count, sperm motility, live sperm count, and increase (p < 0.05) in sperm abnormalities. Activities of gamma-glutamyl transpeptidase, lactate dehydrogenase-x, and sorbitol dehydrogenase were significantly increased (p < 0.05), while, 3β-HSD and 17β-HSD enzymatic activity along with testosterone concentration in testis and plasma were decreased significantly (p < 0.05) in IM-treated rats. IM exposure resulted in significant increase (p < 0.05) in LPO and decrease (p < 0.05) in GSH level along with decreased activities of CAT, SOD, GPx, and GST. IM-treated rats showed histopathological alterations in testis and epididymis. However, the reproductive toxicity parameters, oxidative stress indicators, and histopathological changes were minimized and functional restorations were noticed by co-administration of CMN in IM-treated rats. The results of this study suggest that IM-induced male reproductive toxic effects could be ameliorated by CMN supplementation.",
keywords = "curcumin, imidaclorid, oxidative stress, reproductive toxicity",
author = "Milindmitra Lonare and Manoj Kumar and Sachin Raut and Amar More and Sagar Doltade and Prarabdh Badgujar and Avinash Telang",
year = "2016",
month = "10",
day = "1",
doi = "10.1002/tox.22132",
language = "English (US)",
volume = "31",
pages = "1250--1263",
journal = "Environmental Toxicology",
issn = "1520-4081",
publisher = "John Wiley and Sons Inc.",
number = "10",

}

TY - JOUR

T1 - Evaluation of ameliorative effect of curcumin on imidacloprid-induced male reproductive toxicity in wistar rats

AU - Lonare, Milindmitra

AU - Kumar, Manoj

AU - Raut, Sachin

AU - More, Amar

AU - Doltade, Sagar

AU - Badgujar, Prarabdh

AU - Telang, Avinash

PY - 2016/10/1

Y1 - 2016/10/1

N2 - This study was undertaken to investigate the toxic effects of imidacloprid (IM) on male reproductive system and ameliorative effect of curcumin (CMN) in male Wistar rats. For this purpose, IM (45 and 90 mg/kg, body weight) and CMN (100 mg/kg, body weight) were administered orally to the rats either alone or in combinations for a period of 28 days. At the end of experiment, male reproductive toxicity parameters (total sperm count and sperm abnormalities), testosterone level, steroidal enzymatic activity [3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD], and oxidative stress indicators were estimated in testis and plasma. IM treatments resulted in significant decrease (p < 0.05) in total epididymal sperm count, sperm motility, live sperm count, and increase (p < 0.05) in sperm abnormalities. Activities of gamma-glutamyl transpeptidase, lactate dehydrogenase-x, and sorbitol dehydrogenase were significantly increased (p < 0.05), while, 3β-HSD and 17β-HSD enzymatic activity along with testosterone concentration in testis and plasma were decreased significantly (p < 0.05) in IM-treated rats. IM exposure resulted in significant increase (p < 0.05) in LPO and decrease (p < 0.05) in GSH level along with decreased activities of CAT, SOD, GPx, and GST. IM-treated rats showed histopathological alterations in testis and epididymis. However, the reproductive toxicity parameters, oxidative stress indicators, and histopathological changes were minimized and functional restorations were noticed by co-administration of CMN in IM-treated rats. The results of this study suggest that IM-induced male reproductive toxic effects could be ameliorated by CMN supplementation.

AB - This study was undertaken to investigate the toxic effects of imidacloprid (IM) on male reproductive system and ameliorative effect of curcumin (CMN) in male Wistar rats. For this purpose, IM (45 and 90 mg/kg, body weight) and CMN (100 mg/kg, body weight) were administered orally to the rats either alone or in combinations for a period of 28 days. At the end of experiment, male reproductive toxicity parameters (total sperm count and sperm abnormalities), testosterone level, steroidal enzymatic activity [3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD], and oxidative stress indicators were estimated in testis and plasma. IM treatments resulted in significant decrease (p < 0.05) in total epididymal sperm count, sperm motility, live sperm count, and increase (p < 0.05) in sperm abnormalities. Activities of gamma-glutamyl transpeptidase, lactate dehydrogenase-x, and sorbitol dehydrogenase were significantly increased (p < 0.05), while, 3β-HSD and 17β-HSD enzymatic activity along with testosterone concentration in testis and plasma were decreased significantly (p < 0.05) in IM-treated rats. IM exposure resulted in significant increase (p < 0.05) in LPO and decrease (p < 0.05) in GSH level along with decreased activities of CAT, SOD, GPx, and GST. IM-treated rats showed histopathological alterations in testis and epididymis. However, the reproductive toxicity parameters, oxidative stress indicators, and histopathological changes were minimized and functional restorations were noticed by co-administration of CMN in IM-treated rats. The results of this study suggest that IM-induced male reproductive toxic effects could be ameliorated by CMN supplementation.

KW - curcumin

KW - imidaclorid

KW - oxidative stress

KW - reproductive toxicity

UR - http://www.scopus.com/inward/record.url?scp=84985905675&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84985905675&partnerID=8YFLogxK

U2 - 10.1002/tox.22132

DO - 10.1002/tox.22132

M3 - Article

VL - 31

SP - 1250

EP - 1263

JO - Environmental Toxicology

JF - Environmental Toxicology

SN - 1520-4081

IS - 10

ER -