Evaluation of charging on macromolecules in electron cryomicroscopy

Jacob Brink, Michael Sherman, John Berriman, Wah Chiu

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.

Original languageEnglish (US)
Pages (from-to)41-52
Number of pages12
JournalUltramicroscopy
Volume72
Issue number1-2
DOIs
StatePublished - Apr 1 1998
Externally publishedYes

Fingerprint

Macromolecules
macromolecules
Electron diffraction
charging
Electron irradiation
Electrons
evaluation
Carbon films
Visibility
Diffraction patterns
electrons
electron diffraction
blurring
footprints
electron irradiation
visibility
illuminating
diffraction patterns
expansion
carbon

Keywords

  • Cryomicroscopy
  • Defocused diffraction
  • Electrical breakdown
  • Imaging charged samples
  • Secondary electron emission
  • Specimen current

ASJC Scopus subject areas

  • Materials Science(all)
  • Instrumentation

Cite this

Evaluation of charging on macromolecules in electron cryomicroscopy. / Brink, Jacob; Sherman, Michael; Berriman, John; Chiu, Wah.

In: Ultramicroscopy, Vol. 72, No. 1-2, 01.04.1998, p. 41-52.

Research output: Contribution to journalArticle

Brink, Jacob ; Sherman, Michael ; Berriman, John ; Chiu, Wah. / Evaluation of charging on macromolecules in electron cryomicroscopy. In: Ultramicroscopy. 1998 ; Vol. 72, No. 1-2. pp. 41-52.
@article{969ba15becc44f9981f323f2d747231f,
title = "Evaluation of charging on macromolecules in electron cryomicroscopy",
abstract = "We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.",
keywords = "Cryomicroscopy, Defocused diffraction, Electrical breakdown, Imaging charged samples, Secondary electron emission, Specimen current",
author = "Jacob Brink and Michael Sherman and John Berriman and Wah Chiu",
year = "1998",
month = "4",
day = "1",
doi = "10.1016/S0304-3991(97)00126-5",
language = "English (US)",
volume = "72",
pages = "41--52",
journal = "Ultramicroscopy",
issn = "0304-3991",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Evaluation of charging on macromolecules in electron cryomicroscopy

AU - Brink, Jacob

AU - Sherman, Michael

AU - Berriman, John

AU - Chiu, Wah

PY - 1998/4/1

Y1 - 1998/4/1

N2 - We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.

AB - We describe procedures to assess charging of biological specimens under electron irradiation in an electron cryomicroscope. Charging can be observed by an expansion of the illuminating beam, blurring of electron diffraction patterns and by beam 'footprints' on the specimen. Discharging can also be seen in the defocused electron diffraction mode. We investigated the influence of a variety of factors on the magnitude and visibility of charging. A reduction of charging is noticed when part of the adjacent carbon film is included in the irradiated specimen area.

KW - Cryomicroscopy

KW - Defocused diffraction

KW - Electrical breakdown

KW - Imaging charged samples

KW - Secondary electron emission

KW - Specimen current

UR - http://www.scopus.com/inward/record.url?scp=0032053733&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032053733&partnerID=8YFLogxK

U2 - 10.1016/S0304-3991(97)00126-5

DO - 10.1016/S0304-3991(97)00126-5

M3 - Article

VL - 72

SP - 41

EP - 52

JO - Ultramicroscopy

JF - Ultramicroscopy

SN - 0304-3991

IS - 1-2

ER -