Evaluation of conserved and variable influenza antigens for immunization against different isolates of H5N1 viruses

Ami Patel, Kaylie Tran, Michael Gray, Yan Li, Zhujun Ao, Xiaojian Yao, Darwyn Kobasa, Gary P. Kobinger

Research output: Contribution to journalArticlepeer-review

41 Scopus citations

Abstract

The combination of rapid evolution and high mortality in human cases of infections has raised concerns that the H5N1 avian influenza virus may become a new, possibly severe, pandemic virus. Vaccination is likely to be the most efficient strategy to mitigate the impact of the next influenza pandemic. The present study evaluates B and T cell immune responses generated by the H5N1 viral antigens, hemagglutinin (HA), neuraminidase (NA), nucleoprotein (NP), or the M2 ion channel in parallel, expressed from a DNA vaccine vehicle. Protection studies of immunized mice challenged with 100 LD50 of homologous or heterologous H5N1 viruses indicate that HA afforded better protection than the NA, NP or M2 DNA vaccines. The antibody response was also higher in HA-vaccinated mice as determined by hemagglutination inhibition (HI) and neutralizing antibodies (NAB) assays. Interestingly, the T cell response was higher against HA than against NA, NP or M2 and was detectable at low doses of the DNA-HA vaccine capable of inducing complete protection, despite the absence of a detectable B cell response. This study emphasizes the need to evaluate the relationship between both arms of the adaptive immune responses in regards to protective efficacy against influenza virus.

Original languageEnglish (US)
Pages (from-to)3083-3089
Number of pages7
JournalVaccine
Volume27
Issue number23
DOIs
StatePublished - May 18 2009
Externally publishedYes

Keywords

  • Avian influenza
  • Cell-mediated immunity
  • DNA vaccine
  • H5N1

ASJC Scopus subject areas

  • Molecular Medicine
  • Immunology and Microbiology(all)
  • veterinary(all)
  • Public Health, Environmental and Occupational Health
  • Infectious Diseases

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