Evaluation of RepliVAX WN, a single-cycle flavivirus vaccine, in a non-human primate model of west nile virus infection

Douglas G. Widman, Tomohiro Ishikawa, Luis D. Giavedoni, Vida L. Hodara, Melissa De La Garza, Jessica A. Montalbo, Amelia P. Travassos Da Rosa, Robert B. Tesh, Jean L. Patterson, Ricardo Carrion, Nigel Bourne, Peter W. Mason

Research output: Contribution to journalArticle

28 Citations (Scopus)

Abstract

West Nile virus (WNV) causes serious neurologic disease, but no licensed vaccines are available to prevent this disease in humans. We have developed RepliVAX WN, a single-cycle flavivirus with an expected safety profile superior to other types of live-attenuated viral vaccines. In this report we describe studies examining RepliVAX WN safety, potency, and efficacy in a non-human primate model of WNV infection. A single immunization of four rhesus macaques with RepliVAX WN was safe and elicited detectable neutralizing antibody titers and IgM and IgG responses, and IgG titers were increased in two animals that received a second immunization. After challenge with WNV, three of four immunized animals were completely protected from viremia, and the remaining animal showed minimal viremia on one day. In contrast, the unvaccinated animal developed viremia that lasted six days. These results demonstrate the efficacy and safety of RepliVAX WN in this primate model of WNV infection.

Original languageEnglish (US)
Pages (from-to)1160-1167
Number of pages8
JournalAmerican Journal of Tropical Medicine and Hygiene
Volume82
Issue number6
DOIs
StatePublished - Jun 2010

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Flavivirus
West Nile virus
Virus Diseases
Primates
Viremia
Vaccines
Safety
Immunization
Immunoglobulin G
Viral Vaccines
Attenuated Vaccines
Neutralizing Antibodies
Nervous System Diseases
Macaca mulatta
Immunoglobulin M

ASJC Scopus subject areas

  • Parasitology
  • Infectious Diseases
  • Virology

Cite this

Widman, D. G., Ishikawa, T., Giavedoni, L. D., Hodara, V. L., De La Garza, M., Montalbo, J. A., ... Mason, P. W. (2010). Evaluation of RepliVAX WN, a single-cycle flavivirus vaccine, in a non-human primate model of west nile virus infection. American Journal of Tropical Medicine and Hygiene, 82(6), 1160-1167. https://doi.org/10.4269/ajtmh.2010.09-0310

Evaluation of RepliVAX WN, a single-cycle flavivirus vaccine, in a non-human primate model of west nile virus infection. / Widman, Douglas G.; Ishikawa, Tomohiro; Giavedoni, Luis D.; Hodara, Vida L.; De La Garza, Melissa; Montalbo, Jessica A.; Travassos Da Rosa, Amelia P.; Tesh, Robert B.; Patterson, Jean L.; Carrion, Ricardo; Bourne, Nigel; Mason, Peter W.

In: American Journal of Tropical Medicine and Hygiene, Vol. 82, No. 6, 06.2010, p. 1160-1167.

Research output: Contribution to journalArticle

Widman, DG, Ishikawa, T, Giavedoni, LD, Hodara, VL, De La Garza, M, Montalbo, JA, Travassos Da Rosa, AP, Tesh, RB, Patterson, JL, Carrion, R, Bourne, N & Mason, PW 2010, 'Evaluation of RepliVAX WN, a single-cycle flavivirus vaccine, in a non-human primate model of west nile virus infection', American Journal of Tropical Medicine and Hygiene, vol. 82, no. 6, pp. 1160-1167. https://doi.org/10.4269/ajtmh.2010.09-0310
Widman, Douglas G. ; Ishikawa, Tomohiro ; Giavedoni, Luis D. ; Hodara, Vida L. ; De La Garza, Melissa ; Montalbo, Jessica A. ; Travassos Da Rosa, Amelia P. ; Tesh, Robert B. ; Patterson, Jean L. ; Carrion, Ricardo ; Bourne, Nigel ; Mason, Peter W. / Evaluation of RepliVAX WN, a single-cycle flavivirus vaccine, in a non-human primate model of west nile virus infection. In: American Journal of Tropical Medicine and Hygiene. 2010 ; Vol. 82, No. 6. pp. 1160-1167.
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