Evaluation of simultaneous transmission of chikungunya virus and dengue virus type 2 in infected Aedes aegypti and Aedes albopictus (diptera: Culicidae)

J. T. Nuckols; Y. J.S. Huang; S. Higgs; A. L. Miller; R. B. Pyles; H. M. Spratt; K. M. Horne; D. L. Vanlandingham

doi:10.1093/jme/tjv017

Evaluation of simultaneous transmission of chikungunya virus and dengue virus type 2 in infected Aedes aegypti and Aedes albopictus (diptera: Culicidae)

J. T. Nuckols, Y. J.S. Huang, S. Higgs, A. L. Miller, R. B. Pyles, H. M. Spratt, K. M. Horne, D. L. Vanlandingham

Research output: Contribution to journal › Article › peer-review

48 Scopus citations

Abstract

The simultaneous transmission of chikungunya virus (CHIKV) and dengue viruses (DENV) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. Groups of Aedes aegypti (L.) and Aedes albopictus (Skuse) were orally infected with 1.5 × 10⁵ PFU/ml of CHIKV and 3.2 × 10⁶ FFU/ml of DENV-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qRT-PCR. Simultaneous dissemination of both viruses was detected for all groups in Ae. aegypti and Ae. albopictus while cotransmission of CHIKV and DENV-2 only occurred at low rates after sequential but not simultaneous infection.

Original language	English (US)
Pages (from-to)	447-451
Number of pages	5
Journal	Journal of medical entomology
Volume	52
Issue number	3
DOIs	https://doi.org/10.1093/jme/tjv017
State	Published - May 1 2015

Keywords

Aedes aegypti
Aedes albopictus
Chikungunya
Dengue
Transmission

ASJC Scopus subject areas

Parasitology
General Veterinary
Insect Science
Infectious Diseases

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10.1093/jme/tjv017

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title = "Evaluation of simultaneous transmission of chikungunya virus and dengue virus type 2 in infected Aedes aegypti and Aedes albopictus (diptera: Culicidae)",

abstract = "The simultaneous transmission of chikungunya virus (CHIKV) and dengue viruses (DENV) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. Groups of Aedes aegypti (L.) and Aedes albopictus (Skuse) were orally infected with 1.5 × 105 PFU/ml of CHIKV and 3.2 × 106 FFU/ml of DENV-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qRT-PCR. Simultaneous dissemination of both viruses was detected for all groups in Ae. aegypti and Ae. albopictus while cotransmission of CHIKV and DENV-2 only occurred at low rates after sequential but not simultaneous infection.",

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AU - Nuckols, J. T.

AU - Huang, Y. J.S.

AU - Higgs, S.

AU - Miller, A. L.

AU - Pyles, R. B.

AU - Spratt, H. M.

AU - Horne, K. M.

AU - Vanlandingham, D. L.

PY - 2015/5/1

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N2 - The simultaneous transmission of chikungunya virus (CHIKV) and dengue viruses (DENV) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. Groups of Aedes aegypti (L.) and Aedes albopictus (Skuse) were orally infected with 1.5 × 105 PFU/ml of CHIKV and 3.2 × 106 FFU/ml of DENV-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qRT-PCR. Simultaneous dissemination of both viruses was detected for all groups in Ae. aegypti and Ae. albopictus while cotransmission of CHIKV and DENV-2 only occurred at low rates after sequential but not simultaneous infection.

AB - The simultaneous transmission of chikungunya virus (CHIKV) and dengue viruses (DENV) has been a major public health concern because of their sympatric distribution and shared mosquito vectors. Groups of Aedes aegypti (L.) and Aedes albopictus (Skuse) were orally infected with 1.5 × 105 PFU/ml of CHIKV and 3.2 × 106 FFU/ml of DENV-2 simultaneously or separately in inverse orders and evaluated for dissemination and transmission by qRT-PCR. Simultaneous dissemination of both viruses was detected for all groups in Ae. aegypti and Ae. albopictus while cotransmission of CHIKV and DENV-2 only occurred at low rates after sequential but not simultaneous infection.

KW - Aedes aegypti

KW - Aedes albopictus

KW - Chikungunya

KW - Dengue

KW - Transmission

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Evaluation of simultaneous transmission of chikungunya virus and dengue virus type 2 in infected Aedes aegypti and Aedes albopictus (diptera: Culicidae)

Abstract

Keywords

ASJC Scopus subject areas

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