Evaluation of the mutagenic effects of myosmine in human lymphocytes using the HPRT gene mutation assay

J. B. Havla, C. E. Hill, Sherif Abdel-Rahman, E. Richter

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

The minor tobacco alkaloid myosmine is implicated in DNA damage through pyridyloxobutylation similar to the tobacco-specific nitrosamines (TSNA). In contrast to TSNA, occurrence of myosmine is not restricted to tobacco. Myosmine is genotoxic to human cells in the comet assay. In this study, the mutagenic effect of myosmine was evaluated using the cloning hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene mutation assay. Four hour exposure of isolated peripheral blood lymphocytes from 14 subjects homozygous for the Leu84 wild-type of the O6-methylguanine-DNA-methyltransferase (MGMT) gene to 1 mM of myosmine increased mutant frequency from 0.73 ± 0.58 × 10-6 in control to 1.14 ± 0.89 × 10-6 lymphocytes (P < 0.05). These new data further confirm the mutagenic effects of myosmine.

Original languageEnglish (US)
Pages (from-to)237-241
Number of pages5
JournalFood and Chemical Toxicology
Volume47
Issue number1
DOIs
StatePublished - Jan 2009

Fingerprint

Hypoxanthine Phosphoribosyltransferase
hypoxanthine
Lymphocytes
guanine
Assays
tobacco
lymphocytes
Tobacco
Genes
mutation
nitrosamines
Mutation
assays
Nitrosamines
genes
methyltransferases
DNA damage
molecular cloning
alkaloids
Comet Assay

Keywords

  • HPRT mutation assay
  • Human lymphocytes
  • Myosmine

ASJC Scopus subject areas

  • Food Science
  • Toxicology

Cite this

Evaluation of the mutagenic effects of myosmine in human lymphocytes using the HPRT gene mutation assay. / Havla, J. B.; Hill, C. E.; Abdel-Rahman, Sherif; Richter, E.

In: Food and Chemical Toxicology, Vol. 47, No. 1, 01.2009, p. 237-241.

Research output: Contribution to journalArticle

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