Evaluation of the mutagenic effects of myosmine in human lymphocytes using the HPRT gene mutation assay

J. B. Havla; C. E. Hill; S. Z. Abdel-Rahman; E. Richter

doi:10.1016/j.fct.2008.11.008

Evaluation of the mutagenic effects of myosmine in human lymphocytes using the HPRT gene mutation assay

J. B. Havla, C. E. Hill, S. Z. Abdel-Rahman, E. Richter

Research output: Contribution to journal › Article › peer-review

14 Scopus citations

Abstract

The minor tobacco alkaloid myosmine is implicated in DNA damage through pyridyloxobutylation similar to the tobacco-specific nitrosamines (TSNA). In contrast to TSNA, occurrence of myosmine is not restricted to tobacco. Myosmine is genotoxic to human cells in the comet assay. In this study, the mutagenic effect of myosmine was evaluated using the cloning hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene mutation assay. Four hour exposure of isolated peripheral blood lymphocytes from 14 subjects homozygous for the Leu84 wild-type of the O⁶-methylguanine-DNA-methyltransferase (MGMT) gene to 1 mM of myosmine increased mutant frequency from 0.73 ± 0.58 × 10^-6 in control to 1.14 ± 0.89 × 10^-6 lymphocytes (P < 0.05). These new data further confirm the mutagenic effects of myosmine.

Original language	English (US)
Pages (from-to)	237-241
Number of pages	5
Journal	Food and Chemical Toxicology
Volume	47
Issue number	1
DOIs	https://doi.org/10.1016/j.fct.2008.11.008
State	Published - Jan 2009
Externally published	Yes

Keywords

HPRT mutation assay
Human lymphocytes
Myosmine

ASJC Scopus subject areas

Food Science
Toxicology

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10.1016/j.fct.2008.11.008

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title = "Evaluation of the mutagenic effects of myosmine in human lymphocytes using the HPRT gene mutation assay",

abstract = "The minor tobacco alkaloid myosmine is implicated in DNA damage through pyridyloxobutylation similar to the tobacco-specific nitrosamines (TSNA). In contrast to TSNA, occurrence of myosmine is not restricted to tobacco. Myosmine is genotoxic to human cells in the comet assay. In this study, the mutagenic effect of myosmine was evaluated using the cloning hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene mutation assay. Four hour exposure of isolated peripheral blood lymphocytes from 14 subjects homozygous for the Leu84 wild-type of the O6-methylguanine-DNA-methyltransferase (MGMT) gene to 1 mM of myosmine increased mutant frequency from 0.73 ± 0.58 × 10-6 in control to 1.14 ± 0.89 × 10-6 lymphocytes (P < 0.05). These new data further confirm the mutagenic effects of myosmine.",

keywords = "HPRT mutation assay, Human lymphocytes, Myosmine",

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AU - Havla, J. B.

AU - Hill, C. E.

AU - Abdel-Rahman, S. Z.

AU - Richter, E.

PY - 2009/1

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N2 - The minor tobacco alkaloid myosmine is implicated in DNA damage through pyridyloxobutylation similar to the tobacco-specific nitrosamines (TSNA). In contrast to TSNA, occurrence of myosmine is not restricted to tobacco. Myosmine is genotoxic to human cells in the comet assay. In this study, the mutagenic effect of myosmine was evaluated using the cloning hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene mutation assay. Four hour exposure of isolated peripheral blood lymphocytes from 14 subjects homozygous for the Leu84 wild-type of the O6-methylguanine-DNA-methyltransferase (MGMT) gene to 1 mM of myosmine increased mutant frequency from 0.73 ± 0.58 × 10-6 in control to 1.14 ± 0.89 × 10-6 lymphocytes (P < 0.05). These new data further confirm the mutagenic effects of myosmine.

AB - The minor tobacco alkaloid myosmine is implicated in DNA damage through pyridyloxobutylation similar to the tobacco-specific nitrosamines (TSNA). In contrast to TSNA, occurrence of myosmine is not restricted to tobacco. Myosmine is genotoxic to human cells in the comet assay. In this study, the mutagenic effect of myosmine was evaluated using the cloning hypoxanthine-guanine phosphoribosyltransferase (HPRT) gene mutation assay. Four hour exposure of isolated peripheral blood lymphocytes from 14 subjects homozygous for the Leu84 wild-type of the O6-methylguanine-DNA-methyltransferase (MGMT) gene to 1 mM of myosmine increased mutant frequency from 0.73 ± 0.58 × 10-6 in control to 1.14 ± 0.89 × 10-6 lymphocytes (P < 0.05). These new data further confirm the mutagenic effects of myosmine.

KW - HPRT mutation assay

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Evaluation of the mutagenic effects of myosmine in human lymphocytes using the HPRT gene mutation assay

Abstract

Keywords

ASJC Scopus subject areas

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