Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger

Csaba Szabó, Brian J. Day, Andrew L. Salzman

    Research output: Contribution to journalArticlepeer-review

    210 Scopus citations

    Abstract

    Here we report that the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP) inhibits the oxidation of dihydrorhodamine-123 by peroxynitrite, but does not scavenge nitric oxide (NO). MnTBAP protects against the suppression of mitochondrial respiration in J774 cells exposed to peroxynitrite or to NO donors. MnTBAP and NG-methyl-L-arginine provide additive protective effect against the suppression of respiration in immunostimulated cells. Our data suggest separate contributions of NO and peroxynitrite to the suppression of mitochondrial respiration and support the role of oxidative stress in the expression of the inducible isoform of NO synthase.

    Original languageEnglish (US)
    Pages (from-to)82-86
    Number of pages5
    JournalFEBS Letters
    Volume381
    Issue number1-2
    DOIs
    StatePublished - Feb 26 1996

    Keywords

    • Endotoxin
    • Inflammation
    • Mn(III) porphyrins
    • Nitric oxide
    • Nitric oxide synthase
    • Peroxynitrite
    • Shock
    • Superoxide
    • Superoxide dismutase

    ASJC Scopus subject areas

    • Biophysics
    • Structural Biology
    • Biochemistry
    • Molecular Biology
    • Genetics
    • Cell Biology

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