Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger

Csaba Szabó; Brian J. Day; Andrew L. Salzman

doi:10.1016/0014-5793(96)00087-7

Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger

Csaba Szabó, Brian J. Day, Andrew L. Salzman

Research output: Contribution to journal › Article › peer-review

219 Scopus citations

Abstract

Here we report that the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP) inhibits the oxidation of dihydrorhodamine-123 by peroxynitrite, but does not scavenge nitric oxide (NO). MnTBAP protects against the suppression of mitochondrial respiration in J774 cells exposed to peroxynitrite or to NO donors. MnTBAP and N^G-methyl-L-arginine provide additive protective effect against the suppression of respiration in immunostimulated cells. Our data suggest separate contributions of NO and peroxynitrite to the suppression of mitochondrial respiration and support the role of oxidative stress in the expression of the inducible isoform of NO synthase.

Original language	English (US)
Pages (from-to)	82-86
Number of pages	5
Journal	FEBS Letters
Volume	381
Issue number	1-2
DOIs	https://doi.org/10.1016/0014-5793(96)00087-7
State	Published - Feb 26 1996
Externally published	Yes

Keywords

Endotoxin
Inflammation
Mn(III) porphyrins
Nitric oxide
Nitric oxide synthase
Peroxynitrite
Shock
Superoxide
Superoxide dismutase

ASJC Scopus subject areas

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger. / Szabó, Csaba; Day, Brian J.; Salzman, Andrew L.
In: FEBS Letters, Vol. 381, No. 1-2, 26.02.1996, p. 82-86.

Research output: Contribution to journal › Article › peer-review

Szabó, C, Day, BJ & Salzman, AL 1996, 'Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger', FEBS Letters, vol. 381, no. 1-2, pp. 82-86. https://doi.org/10.1016/0014-5793(96)00087-7

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abstract = "Here we report that the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP) inhibits the oxidation of dihydrorhodamine-123 by peroxynitrite, but does not scavenge nitric oxide (NO). MnTBAP protects against the suppression of mitochondrial respiration in J774 cells exposed to peroxynitrite or to NO donors. MnTBAP and NG-methyl-L-arginine provide additive protective effect against the suppression of respiration in immunostimulated cells. Our data suggest separate contributions of NO and peroxynitrite to the suppression of mitochondrial respiration and support the role of oxidative stress in the expression of the inducible isoform of NO synthase.",

keywords = "Endotoxin, Inflammation, Mn(III) porphyrins, Nitric oxide, Nitric oxide synthase, Peroxynitrite, Shock, Superoxide, Superoxide dismutase",

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AU - Szabó, Csaba

AU - Day, Brian J.

AU - Salzman, Andrew L.

PY - 1996/2/26

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N2 - Here we report that the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP) inhibits the oxidation of dihydrorhodamine-123 by peroxynitrite, but does not scavenge nitric oxide (NO). MnTBAP protects against the suppression of mitochondrial respiration in J774 cells exposed to peroxynitrite or to NO donors. MnTBAP and NG-methyl-L-arginine provide additive protective effect against the suppression of respiration in immunostimulated cells. Our data suggest separate contributions of NO and peroxynitrite to the suppression of mitochondrial respiration and support the role of oxidative stress in the expression of the inducible isoform of NO synthase.

AB - Here we report that the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP) inhibits the oxidation of dihydrorhodamine-123 by peroxynitrite, but does not scavenge nitric oxide (NO). MnTBAP protects against the suppression of mitochondrial respiration in J774 cells exposed to peroxynitrite or to NO donors. MnTBAP and NG-methyl-L-arginine provide additive protective effect against the suppression of respiration in immunostimulated cells. Our data suggest separate contributions of NO and peroxynitrite to the suppression of mitochondrial respiration and support the role of oxidative stress in the expression of the inducible isoform of NO synthase.

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KW - Inflammation

KW - Mn(III) porphyrins

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KW - Superoxide dismutase

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Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger

Abstract

Keywords

ASJC Scopus subject areas

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