Evaluation of the relative contribution of nitric oxide and peroxynitrite to the suppression of mitochondrial respiration in immunostimulated macrophages using a manganese mesoporphyrin superoxide dismutase mimetic and peroxynitrite scavenger

Csaba Szabo, Brian J. Day, Andrew L. Salzman

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

Here we report that the cell-permeable superoxide dismutase mimetic Mn(III)tetrakis (4-benzoic acid) porphyrin (MnTBAP) inhibits the oxidation of dihydrorhodamine-123 by peroxynitrite, but does not scavenge nitric oxide (NO). MnTBAP protects against the suppression of mitochondrial respiration in J774 cells exposed to peroxynitrite or to NO donors. MnTBAP and NG-methyl-L-arginine provide additive protective effect against the suppression of respiration in immunostimulated cells. Our data suggest separate contributions of NO and peroxynitrite to the suppression of mitochondrial respiration and support the role of oxidative stress in the expression of the inducible isoform of NO synthase.

Original languageEnglish (US)
Pages (from-to)82-86
Number of pages5
JournalFEBS Letters
Volume381
Issue number1-2
StatePublished - Feb 26 1996
Externally publishedYes

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Keywords

  • Endotoxin
  • Inflammation
  • Mn(III) porphyrins
  • Nitric oxide
  • Nitric oxide synthase
  • Peroxynitrite
  • Shock
  • Superoxide
  • Superoxide dismutase

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

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