Evaluation of the solid-phase radioimmunoassay for diagnosis of St. Louis encephalitis infection in humans

K. L. Wolff, D. J. Muth, B. W. Hudson, D. W. Trent

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Since the use of highly purified viral proteins in the solid-phase radioimmunoasay (SPRIA) procedure is not practical for routine diagnostic serology, the procedure was adapted for use with the crude antigens now commonly used to diagnose St. Louis encephalitis (SLE) virus infections. SLE and various other alpha- and flavirus antigens were tested by both the SPRIA procedure and conventional serological methods. Standards were established for use of the crude antigens in the SPRIA procedure. Reproducibility of the procedure was similar to that of conventional tests. The procedure rocedure specifically differentiated SLE from clinically similar alphaviruses, but did not differentiate SLE from related flaviviruses very well. SPRIA diagnosis of SLE infections by the immunoglobulin G response in paired sera was good. Generally, the crude antigen SPRIA procedure was as sensitive as the conventional serological test, but not as specific.

Original languageEnglish (US)
Pages (from-to)135-140
Number of pages6
JournalJournal of Clinical Microbiology
Volume14
Issue number2
StatePublished - 1981

Fingerprint

St. Louis Encephalitis
Radioimmunoassay
Antigens
St. Louis Encephalitis Viruses
Alphavirus
Flavivirus
Infectious Encephalitis
Serologic Tests
Viral Proteins
Virus Diseases
Serology
Immunoglobulin G

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Evaluation of the solid-phase radioimmunoassay for diagnosis of St. Louis encephalitis infection in humans. / Wolff, K. L.; Muth, D. J.; Hudson, B. W.; Trent, D. W.

In: Journal of Clinical Microbiology, Vol. 14, No. 2, 1981, p. 135-140.

Research output: Contribution to journalArticle

Wolff, K. L. ; Muth, D. J. ; Hudson, B. W. ; Trent, D. W. / Evaluation of the solid-phase radioimmunoassay for diagnosis of St. Louis encephalitis infection in humans. In: Journal of Clinical Microbiology. 1981 ; Vol. 14, No. 2. pp. 135-140.
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