Evaluation of Transovarial Transmission and Transmissibility of Ehrlichia chaffeensis (Rickettsiales: Anaplasmataceae) in Amblyomma americanum (Acari: Ixodidae)

S. Wesley Long, Xiaofeng Zhang, Jianzhi Zhang, Randall P. Ruble, Pete Teel, Xue Jie Yu

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

It has long been assumed that Ehrlichia chaffeensis (Anderson, Dawson & Wilson), is not transmitted transovarially in the lone star tick vector Amblyomma americanum (L.). To test this hypothesis, three beagle dogs, Canis familiaris (L.) (Carnivora: Canidae), were subcutaneously infected with E. chaffeensis (Arkansas strain). Uninfected nymphal lone star ticks were placed on the infected dogs and allowed to feed to repletion. These nymphal ticks were allowed to molt, and five of five adult female ticks sampled were confirmed to be infected by polymerase chain reaction (PCR). Forty infected adult ticks, the majority of which were female with several males included to stimulate feeding, were then placed on two uninfected dogs. Fourteen females were removed early and the rest were allowed to feed to repletion. After feeding to repletion, the six remaining females detached and two of these females were tested preoviposition, whereas the remaining four were allowed to lay eggs. All six of these fully engorged females tested negative by PCR. The egg clutches laid by four engorged adult females, and the larvae that hatched from these eggs along with larvae from a previously untested egg clutch, were shown to be uninfected by PCR. This seems to support the long-held assumption of lack of transovarial transmission. Also of interest, we found that these PCR-confirmed infected adult ticks reared in the laboratory did not transmit E. chaffeensis to uninfected dogs.

Original languageEnglish (US)
Pages (from-to)1000-1004
Number of pages5
JournalJournal of Medical Entomology
Volume40
Issue number6
StatePublished - Nov 2003

Fingerprint

Anaplasmataceae
Ehrlichia chaffeensis
transovarial transmission
Rickettsiales
Ixodidae
Amblyomma americanum
Ticks
Acari
Dogs
ticks
repletion
polymerase chain reaction
dogs
Polymerase Chain Reaction
egg masses
Eggs
Ovum
Larva
Carnivora
Canidae

Keywords

  • Ehrlichia chaffeensis transmission

ASJC Scopus subject areas

  • Insect Science
  • veterinary(all)

Cite this

Evaluation of Transovarial Transmission and Transmissibility of Ehrlichia chaffeensis (Rickettsiales : Anaplasmataceae) in Amblyomma americanum (Acari: Ixodidae). / Long, S. Wesley; Zhang, Xiaofeng; Zhang, Jianzhi; Ruble, Randall P.; Teel, Pete; Yu, Xue Jie.

In: Journal of Medical Entomology, Vol. 40, No. 6, 11.2003, p. 1000-1004.

Research output: Contribution to journalArticle

Long, S. Wesley ; Zhang, Xiaofeng ; Zhang, Jianzhi ; Ruble, Randall P. ; Teel, Pete ; Yu, Xue Jie. / Evaluation of Transovarial Transmission and Transmissibility of Ehrlichia chaffeensis (Rickettsiales : Anaplasmataceae) in Amblyomma americanum (Acari: Ixodidae). In: Journal of Medical Entomology. 2003 ; Vol. 40, No. 6. pp. 1000-1004.
@article{1904cf33430c43208735eccdf6c1e51d,
title = "Evaluation of Transovarial Transmission and Transmissibility of Ehrlichia chaffeensis (Rickettsiales: Anaplasmataceae) in Amblyomma americanum (Acari: Ixodidae)",
abstract = "It has long been assumed that Ehrlichia chaffeensis (Anderson, Dawson & Wilson), is not transmitted transovarially in the lone star tick vector Amblyomma americanum (L.). To test this hypothesis, three beagle dogs, Canis familiaris (L.) (Carnivora: Canidae), were subcutaneously infected with E. chaffeensis (Arkansas strain). Uninfected nymphal lone star ticks were placed on the infected dogs and allowed to feed to repletion. These nymphal ticks were allowed to molt, and five of five adult female ticks sampled were confirmed to be infected by polymerase chain reaction (PCR). Forty infected adult ticks, the majority of which were female with several males included to stimulate feeding, were then placed on two uninfected dogs. Fourteen females were removed early and the rest were allowed to feed to repletion. After feeding to repletion, the six remaining females detached and two of these females were tested preoviposition, whereas the remaining four were allowed to lay eggs. All six of these fully engorged females tested negative by PCR. The egg clutches laid by four engorged adult females, and the larvae that hatched from these eggs along with larvae from a previously untested egg clutch, were shown to be uninfected by PCR. This seems to support the long-held assumption of lack of transovarial transmission. Also of interest, we found that these PCR-confirmed infected adult ticks reared in the laboratory did not transmit E. chaffeensis to uninfected dogs.",
keywords = "Ehrlichia chaffeensis transmission",
author = "Long, {S. Wesley} and Xiaofeng Zhang and Jianzhi Zhang and Ruble, {Randall P.} and Pete Teel and Yu, {Xue Jie}",
year = "2003",
month = "11",
language = "English (US)",
volume = "40",
pages = "1000--1004",
journal = "Journal of Medical Entomology",
issn = "0022-2585",
publisher = "Entomological Society of America",
number = "6",

}

TY - JOUR

T1 - Evaluation of Transovarial Transmission and Transmissibility of Ehrlichia chaffeensis (Rickettsiales

T2 - Anaplasmataceae) in Amblyomma americanum (Acari: Ixodidae)

AU - Long, S. Wesley

AU - Zhang, Xiaofeng

AU - Zhang, Jianzhi

AU - Ruble, Randall P.

AU - Teel, Pete

AU - Yu, Xue Jie

PY - 2003/11

Y1 - 2003/11

N2 - It has long been assumed that Ehrlichia chaffeensis (Anderson, Dawson & Wilson), is not transmitted transovarially in the lone star tick vector Amblyomma americanum (L.). To test this hypothesis, three beagle dogs, Canis familiaris (L.) (Carnivora: Canidae), were subcutaneously infected with E. chaffeensis (Arkansas strain). Uninfected nymphal lone star ticks were placed on the infected dogs and allowed to feed to repletion. These nymphal ticks were allowed to molt, and five of five adult female ticks sampled were confirmed to be infected by polymerase chain reaction (PCR). Forty infected adult ticks, the majority of which were female with several males included to stimulate feeding, were then placed on two uninfected dogs. Fourteen females were removed early and the rest were allowed to feed to repletion. After feeding to repletion, the six remaining females detached and two of these females were tested preoviposition, whereas the remaining four were allowed to lay eggs. All six of these fully engorged females tested negative by PCR. The egg clutches laid by four engorged adult females, and the larvae that hatched from these eggs along with larvae from a previously untested egg clutch, were shown to be uninfected by PCR. This seems to support the long-held assumption of lack of transovarial transmission. Also of interest, we found that these PCR-confirmed infected adult ticks reared in the laboratory did not transmit E. chaffeensis to uninfected dogs.

AB - It has long been assumed that Ehrlichia chaffeensis (Anderson, Dawson & Wilson), is not transmitted transovarially in the lone star tick vector Amblyomma americanum (L.). To test this hypothesis, three beagle dogs, Canis familiaris (L.) (Carnivora: Canidae), were subcutaneously infected with E. chaffeensis (Arkansas strain). Uninfected nymphal lone star ticks were placed on the infected dogs and allowed to feed to repletion. These nymphal ticks were allowed to molt, and five of five adult female ticks sampled were confirmed to be infected by polymerase chain reaction (PCR). Forty infected adult ticks, the majority of which were female with several males included to stimulate feeding, were then placed on two uninfected dogs. Fourteen females were removed early and the rest were allowed to feed to repletion. After feeding to repletion, the six remaining females detached and two of these females were tested preoviposition, whereas the remaining four were allowed to lay eggs. All six of these fully engorged females tested negative by PCR. The egg clutches laid by four engorged adult females, and the larvae that hatched from these eggs along with larvae from a previously untested egg clutch, were shown to be uninfected by PCR. This seems to support the long-held assumption of lack of transovarial transmission. Also of interest, we found that these PCR-confirmed infected adult ticks reared in the laboratory did not transmit E. chaffeensis to uninfected dogs.

KW - Ehrlichia chaffeensis transmission

UR - http://www.scopus.com/inward/record.url?scp=1642529553&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1642529553&partnerID=8YFLogxK

M3 - Article

C2 - 14765684

AN - SCOPUS:1642529553

VL - 40

SP - 1000

EP - 1004

JO - Journal of Medical Entomology

JF - Journal of Medical Entomology

SN - 0022-2585

IS - 6

ER -