Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses

Mariana Kikuti, Laura B. Tauro, Patrícia S.S. Moreira, Leile Camila J. Nascimento, Moyra M. Portilho, Gúbio C. Soares, Scott C. Weaver, Mitermayer G. Reis, Uriel Kitron, Guilherme S. Ribeiro

Research output: Contribution to journalArticle

Abstract

Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.

Original languageEnglish (US)
Pages (from-to)38-43
Number of pages6
JournalInternational Journal of Infectious Diseases
Volume91
DOIs
StatePublished - Feb 2020

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Chikungunya virus
Dengue Virus
Immunoenzyme Techniques
Immunoglobulin M
Serum
Polymerase Chain Reaction
Dengue
Germany
Brazil
Fever
Zika Virus

Keywords

  • Accuracy
  • Chikungunya virus
  • Diagnostic performance
  • ELISA
  • Enzyme-linked immunosorbent assay
  • Sensitivity and specificity

ASJC Scopus subject areas

  • Microbiology (medical)
  • Infectious Diseases

Cite this

Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. / Kikuti, Mariana; Tauro, Laura B.; Moreira, Patrícia S.S.; Nascimento, Leile Camila J.; Portilho, Moyra M.; Soares, Gúbio C.; Weaver, Scott C.; Reis, Mitermayer G.; Kitron, Uriel; Ribeiro, Guilherme S.

In: International Journal of Infectious Diseases, Vol. 91, 02.2020, p. 38-43.

Research output: Contribution to journalArticle

Kikuti, Mariana ; Tauro, Laura B. ; Moreira, Patrícia S.S. ; Nascimento, Leile Camila J. ; Portilho, Moyra M. ; Soares, Gúbio C. ; Weaver, Scott C. ; Reis, Mitermayer G. ; Kitron, Uriel ; Ribeiro, Guilherme S. / Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses. In: International Journal of Infectious Diseases. 2020 ; Vol. 91. pp. 38-43.
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abstract = "Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0{\%} and 10.3{\%}, while for convalescent samples they were 92.4{\%} and 96.9{\%}, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7{\%} and 90.5{\%}, respectively, and Euroimmun specificities were 88.5{\%} and 83.9{\%}, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.",
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T1 - Evaluation of two commercially available chikungunya virus IgM enzyme-linked immunoassays (ELISA) in a setting of concomitant transmission of chikungunya, dengue and Zika viruses

AU - Kikuti, Mariana

AU - Tauro, Laura B.

AU - Moreira, Patrícia S.S.

AU - Nascimento, Leile Camila J.

AU - Portilho, Moyra M.

AU - Soares, Gúbio C.

AU - Weaver, Scott C.

AU - Reis, Mitermayer G.

AU - Kitron, Uriel

AU - Ribeiro, Guilherme S.

PY - 2020/2

Y1 - 2020/2

N2 - Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.

AB - Objective: To evaluate the diagnostic performance of the Inbios (Seattle, US) and Euroimmun (Luebeck, Germany) chikungunya virus (CHIKV) IgM enzyme-linked immunoassays (ELISAs). Methods: We evaluated the tests’ accuracy on sera from 372 patients enrolled in an acute febrile illness surveillance study performed in Salvador, Brazil from Sept/2014 to Jul/2016, a period of simultaneous CHIKV, dengue (DENV), and Zika (ZIKV) virus transmission. We assessed the sensitivity on acute and paired convalescent sera from RT-PCR-confirmed CHIKV cases (collected at median one and 19 days post-onset of symptoms, respectively), and the specificity on sera of RT-PCR-confirmed DENV and ZIKV cases, and on negative patients. Results: The Inbios and Euroimmun tests’ sensitivities for acute samples were 4.0% and 10.3%, while for convalescent samples they were 92.4% and 96.9%, respectively. Overall, Inbios IgM ELISA specificities for acute and convalescent samples were 97.7% and 90.5%, respectively, and Euroimmun specificities were 88.5% and 83.9%, respectively. Conclusions: Both tests presented high sensitivity for convalescent samples. However, the Euroimmun test returned more equivocal results and presented a slightly lower specificity, which might result in a higher rate of false positives if the test is used in scenarios of low CHIKV transmission, when the chance of CHIKV infection is lower.

KW - Accuracy

KW - Chikungunya virus

KW - Diagnostic performance

KW - ELISA

KW - Enzyme-linked immunosorbent assay

KW - Sensitivity and specificity

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