Evaluation of vascular tone and cardiac contractility in response to silver nanoparticles, using Langendorff rat heart preparation

Alejandro Ramirez-Lee Manuel, Pedro Pablo Martinez-Cuevas, Hector Rosas-Hernandez, Cuauhtémoc Oros-Ovalle, Mariela Bravo-Sanchez, Gabriel Alejandro Martinez-Castañon, Carmen Gonzalez

Research output: Contribution to journalArticlepeer-review

17 Scopus citations

Abstract

Silver nanoparticles (AgNPs) have been widely used because of their antimicrobial properties. However, several reports suggest that AgNPs exposure promote cardiac effects that involve nitric oxide (NO) and oxidative stress (OS). Nevertheless, there are no studies related to AgNPs-induced effects in cardiac physiology. The aim of this study was to evaluate the AgNPs direct actions on coronary vascular tone and cardiac contractility using Langendorff rat heart preparation. Low concentrations of AgNPs (0.1 and 1 μg/mL) increased NO derived from inducible NO-synthase (iNOS), without modifying cardiac parameters. Meanwhile, high concentrations (10 and 100 μg/mL) promoted a sustained vasoconstriction and increased cardiac contractility related to OS, leading to rhabdomyolysis. Furthermore, AgNPs were internalized in the cardiac muscle, hindering classic actions induced by phenylephrine (Phe) and acetylcholine (ACh). These data suggest that AgNPs affect cardiac physiology in function of the concentration and in part of the NO generation, NOS expression and OS.

Original languageEnglish (US)
Pages (from-to)1507-1518
Number of pages12
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume13
Issue number4
DOIs
StatePublished - May 1 2017
Externally publishedYes

Keywords

  • Coronary vascular tone
  • Myocardial contractility
  • Nitric oxide
  • Oxidative stress
  • Silver nanoparticles

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Molecular Medicine
  • Biomedical Engineering
  • General Materials Science
  • Pharmaceutical Science

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